Vanderbilt-Ingram Cancer Center

Publications

• Singleton, CK, Xiong, Y, Kirsten, JH, Pendleton, KP eIF2¿¿ kinases regulate development through the BzpR transcription factor in Dictyostelium discoideum. PLoS One, 7(3), e32500, 2012.
• Bowman, RL, Xiong, Y, Kirsten, JH, Singleton, CK eIF2¿¿ kinases control chalone production in Dictyostelium discoideum. Eukaryot Cell, 10(4), 494-501, 2011.
• Kirsten, JH, Xiong, Y, Davis, CT, Singleton, CK Subcellular localization of ammonium transporters in Dictyostelium discoideum. BMC Cell Biol, 971, 2008.
• Rai, M, Xiong, Y, Singleton, CK Disruption of the ifkA and ifkB genes results in altered cell adhesion, morphological defects and a propensity to form pre-stalk O cells during development of Dictyostelium. Differentiation, 74(9-10), 583-95, 2006.
• Singleton, CK, Kirsten, JH, Dinsmore, CJ Function of ammonium transporter A in the initiation of culmination of development in Dictyostelium discoideum. Eukaryot Cell, 5(7), 991-6, 2006.
• Kirsten, JH, Xiong, Y, Dunbar, AJ, Rai, M, Singleton, CK Ammonium transporter C of Dictyostelium discoideum is required for correct prestalk gene expression and for regulating the choice between slug migration and culmination. Dev Biol, 287(1), 146-56, 2005.
• Peter R. Martin, Charles K. Singleton, and Susanne Hiller-Sturmh?fel "The Role of Thiamine Deficiency in Alcoholic Brain Disease. Alcohol Research & Health.." Alcohol Research & Health, 27134-142, 2004.
• Fang, R, Xiong, Y, Singleton, CK IfkA, a presumptive eIF2 alpha kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size. BMC Dev Biol, 33, 2003.
• Follstaedt, SC, Kirsten, JH, Singleton, CK Temporal and spatial expression of ammonium transporter genes during growth and development of Dictyostelium discoideum. Differentiation, 71(9-10), 557-66, 2003.
• Martin, PR, Singleton, CK, Hiller-Sturmhöfel, S The role of thiamine deficiency in alcoholic brain disease. Alcohol Res Health, 27(2), 134-42, 2003.
• Song, Q, Singleton, CK Mitochondria from cultured cells derived from normal and thiamine-responsive megaloblastic anemia individuals efficiently import thiamine diphosphate. BMC Biochem, 38, 2002.
• Singleton, CK, Martin, PR Molecular mechanisms of thiamine utilization. Curr Mol Med, 1(2), 197-207, 2001.
• Singleton, C. K "Transketolase." Encyclopedia of Molecular Medicine, 3224-322, 2001.
• Wang, J. J-L., Hua, Z., Fentress, H. M., and Singleton, C. K "JNK1 is inactivated during thiamine deficiency-induced apoptosis in human neruoblastoma cells." J. Nutri. Biochem., 11208-215, 2000.
• Ragan, P.W., Singleton, C.K., and Martin, P.R "Brain injury associated with chronic alcoholism." CNS Spectrums, 466-87, 1999.
• Pekovich, S. R., Martin, P. R., Poggi, V. and Singleton, C. K "Sensitivity to thiamine deficiency in cultered human cells is dependent on cell type and is enhanced in cells from thiamine- responsive megaloblastic anemia patients." J. Nutri. Biochem, 9215-222, 1998.
• Singleton, CK, Zinda, MJ, Mykytka, B, Yang, P The histidine kinase dhkC regulates the choice between migrating slugs and terminal differentiation in Dictyostelium discoideum. Dev Biol, 203(2), 345-57, 1998.
• Zinda, MJ, Singleton, CK The hybrid histidine kinase dhkB regulates spore germination in Dictyostelium discoideum. Dev Biol, 196(2), 171-83, 1998.
• Pekovich, SR, Martin, PR, Singleton, CK Thiamine deficiency decreases steady-state transketolase and pyruvate dehydrogenase but not alpha-ketoglutarate dehydrogenase mRNA levels in three human cell types. J Nutr, 128(4), 683-7, 1998.
• Wang, JJ, Martin, PR, Singleton, CK A transketolase assembly defect in a Wernicke-Korsakoff syndrome patient. Alcohol Clin Exp Res, 21(4), 576-80, 1997.
• Wang, JJ, Martin, PR, Singleton, CK Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation. Biochim Biophys Acta, 1341(2), 165-72, 1997.
• Singleton, CK Identification and characterization of the thiamine transporter gene of Saccharomyces cerevisiae. Gene, 199(1-2), 111-21, 1997.
• Wang, J.-L., Martin, P. R., and Singleton, C. K "Aspartate 155 of human transketolase is essential for thiamine pyrophosphate-magnesium binding, and cofactor binding is required for dimer formation." Biochem. et. Biophys. Acta, 1341165-172, 1996.
• Singleton, CK, Wang, JJ, Shan, L, Martin, PR Conserved residues are functionally distinct within transketolases of different species. Biochemistry, 35(49), 15865-9, 1996.
• Pekovich, SR, Martin, PR, Singleton, CK Thiamine pyrophosphate-requiring enzymes are altered during pyrithiamine-induced thiamine deficiency in cultured human lymphoblasts. J Nutr, 126(7), 1791-8, 1996.
• Martin, PR, McCool, BA, Singleton, CK Molecular genetics of transketolase in the pathogenesis of the Wernicke-Korsakoff syndrome. Metab Brain Dis, 10(1), 45-55, 1995.
• Singleton, CK, Pekovich, SR, McCool, BA, Martin, PR The thiamine-dependent hysteretic behavior of human transketolase: implications for thiamine deficiency. J Nutr, 125(2), 189-94, 1995.
• Ken, R, Singleton, CK Redundant regulatory elements account for the developmental control of a ribosomal protein gene of Dictyostelium discoideum. Differentiation, 55(2), 97-103, 1994.
• Martin, PR, Pekovich, SR, McCool, BA, Whetsell, WO, Singleton, CK Thiamine utilization in the pathogenesis of alcohol-induced brain damage. Alcohol Alcohol Suppl, 2273-9, 1994.
• McCool, B. A., Plonk, S. G., Martin, P. R., and Singleton, C. K "Cloning of human transketolase and comparison of the nucleotide sequence of the coding region in Wernicke-Korsakoff and non-Wernicke-Korsakoff individuals." J. Biol. Chem., 2681397-1404, 1993.
• McCool, BA, Plonk, SG, Martin, PR, Singleton, CK Cloning of human transketolase cDNAs and comparison of the nucleotide sequence of the coding region in Wernicke-Korsakoff and non-Wernicke-Korsakoff individuals. J Biol Chem, 268(2), 1397-404, 1993.
• Martin, P.R., McCool, B.A., and Singleton, C.K "Genetic sensitivity to thiamine deficiency and development of alcoholic organic brain disease." Alcoholism: Clin. & Exp. Res., 1731-37, 1993.
• Martin, PR, McCool, BA, Singleton, CK Genetic sensitivity to thiamine deficiency and development of alcoholic organic brain disease. Alcohol Clin Exp Res, 17(1), 31-7, 1993.
• McPherson, CE, Singleton, CK Nutrient-responsive promoter elements of the V4 gene of Dictyostelium discoideum. J Mol Biol, 232(2), 386-96, 1993.
• McPherson, CE, Singleton, CK V4, a gene required for the transition from growth to development in Dictyostelium discoideum. Dev Biol, 150(2), 231-42, 1992.
• Singleton, CK, Delude, RL, Ken, R, Manning, SS, McPherson, CE Structure, expression, and regulation of members of the developmentally controlled V and H gene classes from Dictyostelium. Dev Genet, 12(1-2), 88-97, 1991.
• Singleton, CK Nucleotide sequence of V1, a ribosomal protein gene from Dictyostelium discoideum. Nucleic Acids Res, 17(19), 7989, 1989.
• Singleton, CK, Manning, SS, Ken, R Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum. Nucleic Acids Res, 17(23), 9679-92, 1989.
• Singleton, CK, Gregoli, PA, Manning, SS, Northington, SJ Characterization of genes which are transiently expressed during the preaggregative phase of development of Dictyostelium discoideum. Dev Biol, 129(1), 140-6, 1988.
• Singleton, CK, McPherson, CE, Manning, SS Deactivation of gene expression upon the onset of development in dictyostelium discoideum. Dev Genet, 9(4-5), 327-35, 1988.
• Singleton, CK, Manning, SS, Feng, Y Effect of protein synthesis inhibition on gene expression during early development of Dictyostelium discoideum. Mol Cell Biol, 8(1), 10-6, 1988.
• Singleton, C.K., Delude, R.L., and McPherson, C.E "Characterization of genes which are deactivated upon the onset of development of Dictyostelium discoideum." Dev. Biol., 119433-441, 1987.
• Singleton, C.K "Left-handed helical DNA in solution." Topics in Nucleic Acids Structure 3, (S. Neidle, ed.).Macmillan Publishers LTD, Houndsmill, UK, 163-192, 1987.
• Singleton, CK pPAV, a promoter analysis vector for Dictyostelium discoideum. Nucleic Acids Res, 15(22), 9610, 1987.
• Singleton, C.K "Structural heterogeneity of duplex DNA." Cell Proliferation: Recent Advances, vol. I (A.L. Boynton and H.L. Leffert, eds.). Academic Press, Inc., New York, NY, 417-447, 1985.
• Singleton, C.K., Kilpatrick, M.W., and Wells, R.D "S1 nuclease recognizes DNA conformational junctions between left-handed helical (dT-dG)n .(dC-dA)n and contiguous right-handed sequences." J.Biol. Chem., 2591963-1967, 1984.
• Singleton, C.K., Klysik, J., and Wells, R.D "Conformational flexibility of junctions between contiguous B and Z DNA in supercoiled plasmids." Proc. Natl. Acad. Sci. USA, 802447-2451, 1983.
• Singleton, C.K "Effects of salts, temperature, and stem length on supercoil induced formation of cruciforms." J. Biol. Chem., 2587661-7668, 1983.
• Singleton, C.K., Klysik, J., Stirdivant, S.M., and Wells, R.D "Left-handed Z-DNA is induced by supercoiling under physiological ionic conditions." Nature, 299312 316, 1982.
• Singleton, C.K. and Wells, R.D "The facile generation of covalently closed, circular DNAs of defined negative superhelical densities." Anal. Biochem., 122253-257, 1982.