Christine Marie Eischen, Ph.D.
Professor of Pathology, Microbiology, and Immunology
Professor of Cancer Biology
Vanderbilt University Medical Center
Nashville, TN 37232-2561
The primary focus of the Eischen lab is on tumor initiation. We want to know how does a normal cell become a cancer cell. Specifically, we want to know what are the genes and pathways that contribute to or inhibit cellular transformation. We study the pathways, proteins, and recently, the miRNA that contribute to or inhibit tumor development. Lymphoma is the cancer that we have almost 2 decades of experience studying, and multiple projects utilize this expertise and several mouse models of lymphoma. However, lung, breast, and ovarian carcinomas are also studied in the lab.
Currently, there are three major areas of research focus that overlap in the Eischen lab.
The first area of research focus is on the oncogene Myc. We study Myc and its roles in proliferation, transformation, tumor development, and tumor cell maintenance. A large part of studying Myc is centered on the apoptosis that it causes when overexpressed in normal, untransformed cells, and the transcription it induces to drive proliferation. Myc induces the p53 pathway and suppression of the expression of anti-apoptotic Bcl-2 family members to induce apoptosis in normal cells. Cells acquire alterations that inactivate the ability of Myc to induce apoptosis. Moreover, Myc-induced transcription is still incompletely understood. We discovered a novel regulator of Myc called MTBP. There are four projects that focus on various aspects of Myc-induced tumor development.
The second area of research in the Eischen lab focuses on Mdm2 and Mdmx, two oncogenes best known for their negative regulation of the p53 tumor suppressor, and their roles in tumorigenesis. We discovered that both Mdm2 and Mdmx have p53-independent functions that contribute to tumorigenesis. We determined that Mdm2 and Mdmx regulate double-strand DNA break repair through interaction with Nbs1, a component of the Mre11/Rad50//Nbs1 DNA repair complex. We observed that increased expression of Mdm2 or Mdmx induces genome instability and increases transformation through a mechanism that is independent of p53. We are further characterizing this function with novel mouse models and investigating how this function can be capitalized on for the treatment of malignancies, half of which have inactivated p53. There are two projects that focus on Mdm2 and Mdmx in tumorigenesis.
The third area of research in the Eischen lab centers on the contribution miRNA have in tumor development. miRNA are small non-coding RNA that inhibit protein translation. We are utilizing lymphoma and lung cancer models to study miRNA in cancer. Our investigations focus on oncogene regulation of miRNA and whether they inhibit or contribute to tumor development. We have identified miRNA that are induced by Myc that lead apoptosis to protect cells from transformation. Another project focuses on a miRNA that appears to be a novel driver of lung tumor development. A third project is on miRNA that are altered in cutaneous T cell lymphoma and may serve as novel diagnostic markers for this malignancy.
There are open graduate student and postdoctoral fellowship positions. There are also opportunities for clinical fellows.
- McGirt, LY, Baerenwald, DA, Vonderheid, EC, Eischen, CM Early changes in miRNA expression are predictive of response to extracorporeal photopheresis in cutaneous T-cell lymphoma. J Eur Acad Dermatol Venereol, 2014.
- Adams, CM, Eischen, CM Inactivation of p53 is insufficient to allow B cells and B cell lymphomas to survive without Dicer. Cancer Res, 2014.
- Grieb, BC, Chen, X, Eischen, CM MTBP is Over-expressed in Triple Negative Breast Cancer and Contributes to its Growth and Survival. Mol Cancer Res, 2014.
- Carrillo, AM, Bouska, A, Arrate, MP, Eischen, CM Mdmx promotes genomic instability independent of p53 and Mdm2. Oncogene, 02014.
- Grieb, BC, Gramling, MW, Arrate, MP, Chen, X, Beauparlant, SL, Haines, DS, Xiao, H, Eischen, CM Oncogenic Protein MTBP Interacts with MYC to Promote Tumorigenesis. Cancer Res, 2014.
- Eischen, CM, Lozano, G The Mdm Network and its Regulation of p53 Activities: A Rheostat of Cancer Risk. Hum Mutat, 2014.
- Johnson, VE, Vonderheid, EC, Hess, AD, Eischen, CM, McGirt, LY Genetic markers associated with progression in early mycosis fungoides. J Eur Acad Dermatol Venereol, 2013.
- Wells, CE, Bhaskara, S, Stengel, KR, Zhao, Y, Sirbu, B, Chagot, B, Cortez, D, Khabele, D, Chazin, WJ, Cooper, A, Jacques, V, Rusche, J, Eischen, CM, McGirt, LY, Hiebert, SW Inhibition of histone deacetylase 3 causes replication stress in cutaneous T cell lymphoma. PLoS One, 8(7), e68915, 2013.
- McGirt, LY, Adams, CM, Baerenwald, DA, Zwerner, JP, Zic, JA, Eischen, CM miR-223 Regulates Cell Growth and Targets Proto-Oncogenes in Mycosis Fungoides/Cutaneous T-Cell Lymphoma. J Invest Dermatol, 2013.
- Eischen, CM, Boyd, K Decreased Mdm2 expression inhibits tumor development and extends survival independent of Arf and dependent on p53. PLoS One, 7(9), e46148, 2012.
- Anderson, PD, McKissic, SA, Logan, M, Roh, M, Franco, OE, Wang, J, Doubinskaia, I, van der Meer, R, Hayward, SW, Eischen, CM, Eltoum, IE, Abdulkadir, SA Nkx3.1 and Myc crossregulate shared target genes in mouse and human prostate tumorigenesis. J Clin Invest, 122(5), 1907-19, 2012.
- Melo, AN, Eischen, CM Protecting the genome from mdm2 and mdmx. Genes Cancer, 3(3-4), 283-90, 2012.
- Gramling, MW, Eischen, CM Suppression of Ras/Mapk pathway signaling inhibits Myc-induced lymphomagenesis. Cell Death Differ, 19(7), 1220-7, 2012.
- Lushnikova, T, Bouska, A, Odvody, J, Dupont, WD, Eischen, CM Aging mice have increased chromosome instability that is exacerbated by elevated Mdm2 expression. Oncogene, 2011.
- Cho, SH, Ahn, AK, Bhargava, P, Lee, CH, Eischen, CM, McGuinness, O, Boothby, M Glycolytic rate and lymphomagenesis depend on PARP14, an ADP ribosyltransferase of the B aggressive lymphoma (BAL) family. Proc Natl Acad Sci U S A, 108(38), 15972-7, 2011.
- Odvody, J, Vincent, T, Arrate, MP, Grieb, B, Wang, S, Garriga, J, Lozano, G, Iwakuma, T, Haines, DS, Eischen, CM A deficiency in Mdm2 binding protein inhibits Myc-induced B-cell proliferation and lymphomagenesis. Oncogene, 29(22), 3287-96, 2010.
- Arrate, MP, Vincent, T, Odvody, J, Kar, R, Jones, SN, Eischen, CM MicroRNA biogenesis is required for Myc-induced B-cell lymphoma development and survival. Cancer Res, 70(14), 6083-92, 2010.
- Bouska, A, Eischen, CM Mdm2 Affects Genome Stability Independent of p53. Cancer Res, 69(5), 1697-701, 2009.
- Cho, SH, Goenka, S, Henttinen, T, Gudapati, P, Reinikainen, A, Eischen, CM, Lahesmaa, R, Boothby, M PARP-14, a member of the B aggressive lymphoma (BAL) family, transduces survival signals in primary B cells. Blood, 2009.
- Eischen, CM, Lozano, G p53 and MDM2: Antagonists or Partners in Crime. Cancer Cell, 15(3), 161-2, 2009.
- Mudhasani, R, Zhu, Z, Hutvagner, G, Eischen, CM, Lyle, S, Hall, LL, Lawrence, JB, Imbalzano, AN, Jones, SN Loss of miRNA biogenesis induces p19Arf-p53 signaling and senescence in primary cells. J Cell Biol, 181(7), 1055-63, 2008.
- Bouska, A, Lushnikova, T, Plaza, S, Eischen, CM Mdm2 promotes genetic instability and transformation independent of p53. Mol Cell Biol, 28(15), 4862-74, 2008.
- Wang, P, Lushnikova, T, Odvody, J, Greiner, TC, Jones, SN, Eischen, CM Elevated Mdm2 expression induces chromosomal instability and confers a survival and growth advantage to B cells. Oncogene, 2007.
- Iwakuma, T, Tochigi, Y, Van Pelt, CS, Caldwell, LC, Terzian, T, Parant, JM, Chau, GP, Koch, JG, Eischen, CM, Lozano, G Mtbp haploinsufficiency in mice increases tumor metastasis. Oncogene, 2007.
- Wang, P, Greiner, TC, Lushnikova, T, Eischen, CM Decreased Mdm2 expression inhibits tumor development induced by loss of ARF. Oncogene, 25(26), 3708-18, 2006.
- Alt, JR, Bouska, A, Fernandez, MR, Cerny, RL, Xiao, H, Eischen, CM Mdm2 binds to Nbs1 at sites of DNA damage and regulates double strand break repair. J Biol Chem, 280(19), 18771-81, 2005.
- Eischen, CM, Alt, JR, Wang, P Loss of one allele of ARF rescues Mdm2 haploinsufficiency effects on apoptosis and lymphoma development. Oncogene, 23(55), 8931-40, 2004.
- Alt, JR, Greiner, TC, Cleveland, JL, Eischen, CM Mdm2 haplo-insufficiency profoundly inhibits Myc-induced lymphomagenesis. EMBO J, 22(6), 1442-50, 2003.
- Eischen, CM, Rehg, JE, Korsmeyer, SJ, Cleveland, JL Loss of Bax alters tumor spectrum and tumor numbers in ARF-deficient mice. Cancer Res, 62(7), 2184-91, 2002.
- Eischen, CM, Woo, D, Roussel, MF, Cleveland, JL Apoptosis triggered by Myc-induced suppression of Bcl-X(L) or Bcl-2 is bypassed during lymphomagenesis. Mol Cell Biol, 21(15), 5063-70, 2001.
- Eischen, CM, Roussel, MF, Korsmeyer, SJ, Cleveland, JL Bax loss impairs Myc-induced apoptosis and circumvents the selection of p53 mutations during Myc-mediated lymphomagenesis. Mol Cell Biol, 21(22), 7653-62, 2001.
- Eischen, CM, Packham, G, Nip, J, Fee, BE, Hiebert, SW, Zambetti, GP, Cleveland, JL Bcl-2 is an apoptotic target suppressed by both c-Myc and E2F-1. Oncogene, 20(48), 6983-93, 2001.
- Nip, J, Strom, DK, Eischen, CM, Cleveland, JL, Zambetti, GP, Hiebert, SW E2F-1 induces the stabilization of p53 but blocks p53-mediated transactivation. Oncogene, 20(8), 910-20, 2001.
- Eischen, CM, Weber, JD, Roussel, MF, Sherr, CJ, Cleveland, JL Disruption of the ARF-Mdm2-p53 tumor suppressor pathway in Myc-induced lymphomagenesis. Genes Dev, 13(20), 2658-69, 1999.
- Zindy, F, Eischen, CM, Randle, DH, Kamijo, T, Cleveland, JL, Sherr, CJ, Roussel, MF Myc signaling via the ARF tumor suppressor regulates p53-dependent apoptosis and immortalization. Genes Dev, 12(15), 2424-33, 1998.
- Eischen, CM, Kottke, TJ, Martins, LM, Basi, GS, Tung, JS, Earnshaw, WC, Leibson, PJ, Kaufmann, SH Comparison of apoptosis in wild-type and Fas-resistant cells: chemotherapy-induced apoptosis is not dependent on Fas/Fas ligand interactions. Blood, 90(3), 935-43, 1997.
- Eischen, CM, Williams, BL, Zhang, W, Samelson, LE, Lynch, DH, Abraham, RT, Leibson, PJ ZAP-70 tyrosine kinase is required for the up-regulation of Fas ligand in activation-induced T cell apoptosis. J Immunol, 159(3), 1135-9, 1997.
- Eischen, CM, Schilling, JD, Lynch, DH, Krammer, PH, Leibson, PJ Fc receptor-induced expression of Fas ligand on activated NK cells facilitates cell-mediated cytotoxicity and subsequent autocrine NK cell apoptosis. J Immunol, 156(8), 2693-9, 1996.