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David K.  Cortez

David K. Cortez, Ph.D.

Co-Leader, Genome Maintenance Research Program
Ingram Professor of Cancer Research
Professor of Biochemistry
Researcher

Contact Information:

Vanderbilt University Medical Center
613 Light Hall
Nashville, TN 37232-0146
615-322-8547

Research Description

Billions of base pairs of DNA must be replicated trillions of times during a human lifetime. Adding to the difficulty, thousands of DNA lesions happen in each cell of our body every day. Furthermore, replication is challenged by difficult to replicate sequences and conflicts with transcription. DNA damage response mechanisms act to repair the damaged DNA, signal checkpoint activation, ensure completion of DNA replication, and maintain genome stability. Defects in these mechanisms can cause developmental abnormalities, premature aging, and cancer.

The Cortez lab is dedicated to understanding the mechanisms that maintain genome integrity and promote the complete and accurate replication of the genome.

Current projects in the lab include:

1. Identification of new replication stress response proteins using whole genome RNAi screens and proteomics
2. Characterization of replication fork remodeling enzymes including SMARCAL1
3. Analysis of DNA damage signaling pathways controlled by the ATR kinase
4. Development of cancer therapeutic approaches targeting the DNA damage response

We use a multi-disciplinary approach including genetics, biochemistry, cell biology, proteomics, and structural biology. The lab is part of the Genome Maintenance Program within the Vanderbilt-Ingram Cancer Center, Center in Molecular Toxicology, Vanderbilt Institute of Chemical Biology, and Department of Biochemistry.

SIGNIFICANCE
Since DNA damage is continuously produced as a byproduct of normal cell metabolism and DNA replication, any deficiency in responding to and repairing this damage can cause chromosomal alterations that may lead to cancer, developmental abnormalities, and other diseases. In addition many cancer therapies including radiation therapy and most chemotherapeutic strategies cause DNA damage. Therefore, manipulating the DNA damage response may be one means of improving the outcomes of these therapies. Discovering the mechanisms by which cells respond to DNA damage and other types of replication stress will provide insights into the etiology of diseases like cancer as well as identify opportunities for therapeutic intervention.


. . .

Publications
  • Bass Thomas, Luzwick Jessica, Kavanaugh Gina, Carroll Clinton, Dungrawala Huzefa, Glick Gloria, Feldkamp Michael, Putney Reid, Chazin Walter, Cortez David. ETAA1 acts at stalled replication forks to maintain genome integrity. Nature Cell Biology. 2016 Oct 10/10/2016; PMID: 27723720.
    Available from: http://www.nature.com/ncb/journal/vaop/ncurrent/full/ncb3415.html.
  • Chaudhuri Arnab Ray, Callen Elsa, Ding Xia, Cortez David, Cantor Sharon, Fernandez-Capetillo Oscar, Ge Kai, Jonkers Jos, Rlottenberg Sven, Sharan Shyam, Nussenzweig Andre, et al. Replication fork stability confers chemoresistance in BRCA-deficient cells. Nature. 2016 Jul 7/20/2016; 535: 382-7. PMID: 27443740.
    Available from: http://www.nature.com/nature/journal/v535/n7612/full/nature18325.html.
  • Badu-Nkansah Akosua, Mason Aaron, Eichman Brandt, Cortez David. Identification of a Substrate Recognition Domain in the Replication Stress Response Protein Zinc Finger Ran-binding Domain-containing Protein 3 (ZRANB3). J Biol Chem. 2016 Feb 2/16/2016; 291(15): 8251-7. PMID: 26884333.
    Available from: http://www.jbc.org/content/291/15/8251.long.
  • Poole LA, Zhao R, Glick GG, Lovejoy CA, Eischen CM, Cortez D. SMARCAL1 maintains telomere integrity during DNA replication. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Dec 12/1/2015; 112(48): 14864-9. PMID: 26578802, PMCID: PMC4672769, PII: 1510750112, DOI: 10.1073/pnas.1510750112, ISSN: 1091-6490.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/26578802.
  • Cortez D, Zhou Z, Sanchez Y. Stephen Elledge and the DNA damage response. DNA Repair (Amst.). 2015 Nov; 35: 156-7. PMID: 26574138, PII: S1568-7864(15)00216-5, DOI: 10.1016/j.dnarep.2015.11.001, ISSN: 1568-7856.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/26574138.
  • Kavanaugh G, Ye F, Mohni KN, Luzwick JW, Glick G, Cortez D. A whole genome RNAi screen identifies replication stress response genes. DNA Repair (Amst.) [print-electronic]. 2015 Nov; 35: 55-62. PMID: 26454783, PMCID: PMC4651756, PII: S1568-7864(15)30004-5, DOI: 10.1016/j.dnarep.2015.09.024, ISSN: 1568-7856.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/26454783.
  • Carroll C, Hunley TE, Guo Y, Cortez D. A novel splice site mutation in SMARCAL1 results in aberrant exon definition in a child with schimke immunoosseous dysplasia. Am. J. Med. Genet. A [print-electronic]. 2015 Oct; 167A(10): 2260-4. PMID: 25943327, DOI: 10.1002/ajmg.a.37146, ISSN: 1552-4833.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25943327.
  • Dungrawala H, Rose KL, Bhat KP, Mohni KN, Glick GG, Couch FB, Cortez D. The Replication Checkpoint Prevents Two Types of Fork Collapse without Regulating Replisome Stability. Mol. Cell [print-electronic]. 2015 Sep 9/17/2015; 59(6): 998-1010. PMID: 26365379, PMCID: PMC4575883, PII: S1097-2765(15)00588-2, DOI: 10.1016/j.molcel.2015.07.030, ISSN: 1097-4164.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/26365379.
  • Kavanaugh G, Zhao R, Guo Y, Mohni KN, Glick G, Lacy ME, Hutson MS, Ascano M, Cortez D. Enhancer of Rudimentary Homolog Affects the Replication Stress Response through Regulation of RNA Processing. Mol. Cell. Biol [print-electronic]. 2015 Sep 9/1/2015; 35(17): 2979-90. PMID: 26100022, PMCID: PMC4525316, PII: MCB.01276-14, DOI: 10.1128/MCB.01276-14, ISSN: 1098-5549.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/26100022.
  • Cortez D. Preventing replication fork collapse to maintain genome integrity. DNA Repair (Amst.) [print-electronic]. 2015 Aug; 32: 149-57. PMID: 25957489, PMCID: PMC4522347, PII: S1568-7864(15)00118-4, DOI: 10.1016/j.dnarep.2015.04.026, ISSN: 1568-7856.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25957489.
  • Bhat KP, Bétous R, Cortez D. High-affinity DNA-binding domains of replication protein A (RPA) direct SMARCAL1-dependent replication fork remodeling. J. Biol. Chem [print-electronic]. 2015 Feb 2/13/2015; 290(7): 4110-7. PMID: 25552480, PMCID: PMC4326820, PII: M114.627083, DOI: 10.1074/jbc.M114.627083, ISSN: 1083-351X.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25552480.
  • Dungrawala H, Cortez D. Purification of proteins on newly synthesized DNA using iPOND. Methods Mol. Biol. 2015; 1228: 123-31. PMID: 25311126, PMCID: PMC4384176, DOI: 10.1007/978-1-4939-1680-1_10, ISSN: 1940-6029.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25311126.
  • Mohni KN, Thompson PS, Luzwick JW, Glick GG, Pendleton CS, Lehmann BD, Pietenpol JA, Cortez D. A Synthetic Lethal Screen Identifies DNA Repair Pathways that Sensitize Cancer Cells to Combined ATR Inhibition and Cisplatin Treatments. PLoS ONE. 2015; 10(5): e0125482. PMID: 25965342, PMCID: PMC4428765, PII: PONE-D-14-51553, DOI: 10.1371/journal.pone.0125482, ISSN: 1932-6203.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25965342.
  • Ning B, Feldkamp MD, Cortez D, Chazin WJ, Friedman KL, Fanning E. Simian virus Large T antigen interacts with the N-terminal domain of the 70 kD subunit of Replication Protein A in the same mode as multiple DNA damage response factors. PLoS ONE. 2015; 10(2): e0116093. PMID: 25706313, PMCID: PMC4337903, PII: PONE-D-14-45260, DOI: 10.1371/journal.pone.0116093, ISSN: 1932-6203.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25706313.
  • Sowd GA, Mody D, Eggold J, Cortez D, Friedman KL, Fanning E. SV40 utilizes ATM kinase activity to prevent non-homologous end joining of broken viral DNA replication products. PLoS Pathog. 2014 Dec; 10(12): e1004536. PMID: 25474690, PMCID: PMC4256475, PII: PPATHOGENS-D-14-01844, DOI: 10.1371/journal.ppat.1004536, ISSN: 1553-7374.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25474690.
  • Mason AC, Rambo RP, Greer B, Pritchett M, Tainer JA, Cortez D, Eichman BF. A structure-specific nucleic acid-binding domain conserved among DNA repair proteins. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2014 May 5/27/2014; 111(21): 7618-23. PMID: 24821763, PMCID: PMC4040553, PII: 1324143111, DOI: 10.1073/pnas.1324143111, ISSN: 1091-6490.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/24821763.
  • Mohni KN, Kavanaugh GM, Cortez D. ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency. Cancer Res [print-electronic]. 2014 May 5/15/2014; 74(10): 2835-45. PMID: 24662920, PMCID: PMC4043842, PII: 0008-5472.CAN-13-3229, DOI: 10.1158/0008-5472.CAN-13-3229, ISSN: 1538-7445.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/24662920.
  • Frank AO, Vangamudi B, Feldkamp MD, Souza-Fagundes EM, Luzwick JW, Cortez D, Olejniczak ET, Waterson AG, Rossanese OW, Chazin WJ, Fesik SW. Discovery of a potent stapled helix peptide that binds to the 70N domain of replication protein A. J. Med. Chem [print-electronic]. 2014 Mar 3/27/2014; 57(6): 2455-61. PMID: 24491171, PMCID: PMC3969094, DOI: 10.1021/jm401730y, ISSN: 1520-4804.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/24491171.
  • Carroll C, Bansbach CE, Zhao R, Jung SY, Qin J, Cortez D. Phosphorylation of a C-terminal auto-inhibitory domain increases SMARCAL1 activity. Nucleic Acids Res [print-electronic]. 2014 Jan; 42(2): 918-25. PMID: 24150942, PMCID: PMC3902923, PII: gkt929, DOI: 10.1093/nar/gkt929, ISSN: 1362-4962.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/24150942.
  • Couch FB, Cortez D. Fork reversal, too much of a good thing [editorial]. Cell Cycle [print-electronic]. 2014; 13(7): 1049-50. PMID: 24553113, PMCID: PMC4013150, PII: 28212, DOI: 10.4161/cc.28212, ISSN: 1551-4005.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/24553113.
  • Luzwick JW, Nam EA, Zhao R, Cortez D. Mutation of serine 1333 in the ATR HEAT repeats creates a hyperactive kinase. PLoS ONE. 2014; 9(6): e99397. PMID: 24901225, PMCID: PMC4047089, PII: PONE-D-14-10562, DOI: 10.1371/journal.pone.0099397, ISSN: 1932-6203.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/24901225.
  • Carroll C, Badu-Nkansah A, Hunley T, Baradaran-Heravi A, Cortez D, Frangoul H. Schimke Immunoosseous Dysplasia associated with undifferentiated carcinoma and a novel SMARCAL1 mutation in a child. Pediatr Blood Cancer [print-electronic]. 2013 Sep; 60(9): E88-90. PMID: 23630135, PMCID: PMC3713188, DOI: 10.1002/pbc.24542, ISSN: 1545-5017.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/23630135.
  • Sirbu BM, Cortez D. DNA damage response: three levels of DNA repair regulation. Cold Spring Harb Perspect Biol. 2013 Aug; 5(8): a012724. PMID: 23813586, PMCID: PMC3721278, PII: cshperspect.a012724, DOI: 10.1101/cshperspect.a012724, ISSN: 1943-0264.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/23813586.
  • Couch FB, Bansbach CE, Driscoll R, Luzwick JW, Glick GG, Bétous R, Carroll CM, Jung SY, Qin J, Cimprich KA, Cortez D. ATR phosphorylates SMARCAL1 to prevent replication fork collapse. Genes Dev. 2013 Jul 7/15/2013; 27(14): 1610-23. PMID: 23873943, PMCID: PMC3731549, PII: 27/14/1610, DOI: 10.1101/gad.214080.113, ISSN: 1549-5477.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/23873943.
  • Summers AR, Fischer MA, Stengel KR, Zhao Y, Kaiser JF, Wells CE, Hunt A, Bhaskara S, Luzwick JW, Sampathi S, Chen X, Thompson MA, Cortez D, Hiebert SW. HDAC3 is essential for DNA replication in hematopoietic progenitor cells. J. Clin. Invest [print-electronic]. 2013 Jul; 123(7): 3112-23. PMID: 23921131, PMCID: PMC3696547, PII: 60806, DOI: 10.1172/JCI60806, ISSN: 1558-8238.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/23921131.
  • Bétous R, Couch FB, Mason AC, Eichman BF, Manosas M, Cortez D. Substrate-selective repair and restart of replication forks by DNA translocases. Cell Rep [print-electronic]. 2013 Jun 6/27/2013; 3(6): 1958-69. PMID: 23746452, PMCID: PMC3700663, PII: S2211-1247(13)00219-2, DOI: 10.1016/j.celrep.2013.05.002, ISSN: 2211-1247.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/23746452.
  • Bétous R, Glick GG, Zhao R, Cortez D. Identification and characterization of SMARCAL1 protein complexes. PLoS ONE [electronic-print]. 2013; 8(5): e63149. PMID: 23671665, PMCID: PMC3650004, PII: PONE-D-13-02305, DOI: 10.1371/journal.pone.0063149, ISSN: 1932-6203.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/23671665.
  • Wells CE, Bhaskara S, Stengel KR, Zhao Y, Sirbu B, Chagot B, Cortez D, Khabele D, Chazin WJ, Cooper A, Jacques V, Rusche J, Eischen CM, McGirt LY, Hiebert SW. Inhibition of histone deacetylase 3 causes replication stress in cutaneous T cell lymphoma. PLoS ONE [electronic-print]. 2013; 8(7): e68915. PMID: 23894374, PMCID: PMC3718806, PII: PONE-D-13-03947, DOI: 10.1371/journal.pone.0068915, ISSN: 1932-6203.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/23894374.
  • Sirbu BM, Couch FB, Cortez D. Monitoring the spatiotemporal dynamics of proteins at replication forks and in assembled chromatin using isolation of proteins on nascent DNA. Nat Protoc. 2012 Mar; 7(3): 594-605. PMID: 22383038, PMCID: PMC3671908, PII: nprot.2012.010, DOI: 10.1038/nprot.2012.010, ISSN: 1750-2799.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/22383038.
  • Bétous R, Mason AC, Rambo RP, Bansbach CE, Badu-Nkansah A, Sirbu BM, Eichman BF, Cortez D. SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication. Genes Dev. 2012 Jan 1/15/2012; 26(2): 151-62. PMID: 22279047, PMCID: PMC3273839, PII: 26/2/151, DOI: 10.1101/gad.178459.111, ISSN: 1549-5477.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/22279047.
  • Nam EA, Zhao R, Cortez D. Analysis of mutations that dissociate G(2) and essential S phase functions of human ataxia telangiectasia-mutated and Rad3-related (ATR) protein kinase. J. Biol. Chem [print-electronic]. 2011 Oct 10/28/2011; 286(43): 37320-7. PMID: 21908846, PMCID: PMC3199479, PII: M111.276113, DOI: 10.1074/jbc.M111.276113, ISSN: 1083-351X.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21908846.
  • Nam EA, Zhao R, Glick GG, Bansbach CE, Friedman DB, Cortez D. Thr-1989 phosphorylation is a marker of active ataxia telangiectasia-mutated and Rad3-related (ATR) kinase. J. Biol. Chem [print-electronic]. 2011 Aug 8/19/2011; 286(33): 28707-14. PMID: 21705319, PMCID: PMC3190678, PII: M111.248914, DOI: 10.1074/jbc.M111.248914, ISSN: 1083-351X.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21705319.
  • Bansbach CE, Cortez D. Defining genome maintenance pathways using functional genomic approaches. Crit. Rev. Biochem. Mol. Biol. 2011 Aug; 46(4): 327-41. PMID: 21787120, PMCID: PMC3144553, DOI: 10.3109/10409238.2011.588938, ISSN: 1549-7798.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21787120.
  • Nam EA, Cortez D. ATR signalling: more than meeting at the fork. Biochem. J. 2011 Jun 6/15/2011; 436(3): 527-36. PMID: 21615334, PMCID: PMC3678388, PII: BJ20102162, DOI: 10.1042/BJ20102162, ISSN: 1470-8728.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21615334.
  • Sirbu BM, Couch FB, Feigerle JT, Bhaskara S, Hiebert SW, Cortez D. Analysis of protein dynamics at active, stalled, and collapsed replication forks. Genes Dev. 2011 Jun 6/15/2011; 25(12): 1320-7. PMID: 21685366, PMCID: PMC3127432, PII: 25/12/1320, DOI: 10.1101/gad.2053211, ISSN: 1549-5477.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21685366.
  • Yu DS, Zhao R, Hsu EL, Cayer J, Ye F, Guo Y, Shyr Y, Cortez D. Cyclin-dependent kinase 9-cyclin K functions in the replication stress response. EMBO Rep [print-electronic]. 2010 Nov; 11(11): 876-82. PMID: 20930849, PMCID: PMC2966956, PII: embor2010153, DOI: 10.1038/embor.2010.153, ISSN: 1469-3178.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/20930849.
  • Wiltshire TD, Lovejoy CA, Wang T, Xia F, O'Connor MJ, Cortez D. Sensitivity to poly(ADP-ribose) polymerase (PARP) inhibition identifies ubiquitin-specific peptidase 11 (USP11) as a regulator of DNA double-strand break repair. J. Biol. Chem [print-electronic]. 2010 May 5/7/2010; 285(19): 14565-71. PMID: 20233726, PMCID: PMC2863164, PII: M110.104745, DOI: 10.1074/jbc.M110.104745, ISSN: 1083-351X.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/20233726.
  • Bansbach CE, Boerkoel CF, Cortez D. SMARCAL1 and replication stress: an explanation for SIOD?. Nucleus [print-electronic]. 2010 May; 1(3): 245-8. PMID: 21327070, PMCID: PMC3027029, DOI: 10.4161/nucl.1.3.11739, ISSN: 1949-1042.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21327070.
  • Lovejoy CA, Xu X, Bansbach CE, Glick GG, Zhao R, Ye F, Sirbu BM, Titus LC, Shyr Y, Cortez D. Functional genomic screens identify CINP as a genome maintenance protein. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2009 Nov 11/17/2009; 106(46): 19304-9. PMID: 19889979, PMCID: PMC2780779, PII: 0909345106, DOI: 10.1073/pnas.0909345106, ISSN: 1091-6490.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/19889979.
  • Bansbach CE, Bétous R, Lovejoy CA, Glick GG, Cortez D. The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forks. Genes Dev [print-electronic]. 2009 Oct 10/15/2009; 23(20): 2405-14. PMID: 19793861, PMCID: PMC2764496, PII: gad.1839909, DOI: 10.1101/gad.1839909, ISSN: 1549-5477.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/19793861.
  • Lovejoy CA, Cortez D. Common mechanisms of PIKK regulation. DNA Repair (Amst.) [print-electronic]. 2009 Sep 9/2/2009; 8(9): 1004-8. PMID: 19464237, PMCID: PMC2725225, PII: S1568-7864(09)00100-1, DOI: 10.1016/j.dnarep.2009.04.006, ISSN: 1568-7856.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/19464237.
  • Nam EA, Cortez D. SOSS1/2: Sensors of single-stranded DNA at a break. Mol. Cell. 2009 Aug 8/14/2009; 35(3): 258-9. PMID: 19683490, PMCID: PMC2820400, PII: S1097-2765(09)00512-7, DOI: 10.1016/j.molcel.2009.07.016, ISSN: 1097-4164.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/19683490.
  • Mordes DA, Nam EA, Cortez D. Dpb11 activates the Mec1-Ddc2 complex. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Dec 12/2/2008; 105(48): 18730-4. PMID: 19028869, PMCID: PMC2596233, PII: 0806621105, DOI: 10.1073/pnas.0806621105, ISSN: 1091-6490.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/19028869.
  • Xu X, Vaithiyalingam S, Glick GG, Mordes DA, Chazin WJ, Cortez D. The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling. Mol. Cell. Biol [print-electronic]. 2008 Dec; 28(24): 7345-53. PMID: 18936170, PMCID: PMC2593429, PII: MCB.01079-08, DOI: 10.1128/MCB.01079-08, ISSN: 1098-5549.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/18936170.
  • Mordes DA, Cortez D. Activation of ATR and related PIKKs. Cell Cycle [print-electronic]. 2008 Sep 9/15/2008; 7(18): 2809-12. PMID: 18769153, PMCID: PMC2672405, PII: 6689, ISSN: 1551-4005.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/18769153.
  • Cimprich KA, Cortez D. ATR: an essential regulator of genome integrity. Nat. Rev. Mol. Cell Biol [print-electronic]. 2008 Aug; 9(8): 616-27. PMID: 18594563, PMCID: PMC2663384, PII: nrm2450, DOI: 10.1038/nrm2450, ISSN: 1471-0080.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/18594563.
  • Mordes DA, Glick GG, Zhao R, Cortez D. TopBP1 activates ATR through ATRIP and a PIKK regulatory domain. Genes Dev. 2008 Jun 6/1/2008; 22(11): 1478-89. PMID: 18519640, PMCID: PMC2418584, PII: 22/11/1478, DOI: 10.1101/gad.1666208, ISSN: 0890-9369.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/18519640.
  • Bhaskara S, Chyla BJ, Amann JM, Knutson SK, Cortez D, Sun ZW, Hiebert SW. Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control. Mol. Cell. 2008 Apr 4/11/2008; 30(1): 61-72. PMID: 18406327, PMCID: PMC2373760, PII: S1097-2765(08)00207-4, DOI: 10.1016/j.molcel.2008.02.030, ISSN: 1097-4164.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/18406327.
  • Myers JS, Zhao R, Xu X, Ham AJ, Cortez D. Cyclin-dependent kinase 2 dependent phosphorylation of ATRIP regulates the G2-M checkpoint response to DNA damage. Cancer Res. 2007 Jul 7/15/2007; 67(14): 6685-90. PMID: 17638878, PMCID: PMC2728292, PII: 67/14/6685, DOI: 10.1158/0008-5472.CAN-07-0495, ISSN: 0008-5472.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/17638878.
  • Chen X, Zhao R, Glick GG, Cortez D. Function of the ATR N-terminal domain revealed by an ATM/ATR chimera. Exp. Cell Res [print-electronic]. 2007 May 5/1/2007; 313(8): 1667-74. PMID: 17376433, PMCID: PMC1855264, PII: S0014-4827(07)00084-5, DOI: 10.1016/j.yexcr.2007.02.015, ISSN: 0014-4827.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/17376433.
  • Ball HL, Ehrhardt MR, Mordes DA, Glick GG, Chazin WJ, Cortez D. Function of a conserved checkpoint recruitment domain in ATRIP proteins. Mol. Cell. Biol [print-electronic]. 2007 May; 27(9): 3367-77. PMID: 17339343, PMCID: PMC1899971, PII: MCB.02238-06, DOI: 10.1128/MCB.02238-06, ISSN: 0270-7306.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/17339343.
  • Lovejoy CA, Lock K, Yenamandra A, Cortez D. DDB1 maintains genome integrity through regulation of Cdt1. Mol. Cell. Biol [print-electronic]. 2006 Nov; 26(21): 7977-90. PMID: 16940174, PMCID: PMC1636754, PII: MCB.00819-06, DOI: 10.1128/MCB.00819-06, ISSN: 0270-7306.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/16940174.
  • Myers JS, Cortez D. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J. Biol. Chem [print-electronic]. 2006 Apr 4/7/2006; 281(14): 9346-50. PMID: 16431910, PMCID: PMC1821075, PII: M513265200, DOI: 10.1074/jbc.M513265200, ISSN: 0021-9258.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/16431910.
  • Ball HL, Cortez D. ATRIP oligomerization is required for ATR-dependent checkpoint signaling. J. Biol. Chem [print-electronic]. 2005 Sep 9/9/2005; 280(36): 31390-6. PMID: 16027118, PMCID: PMC1360181, PII: M504961200, DOI: 10.1074/jbc.M504961200, ISSN: 0021-9258.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/16027118.
  • Cortez D. Unwind and slow down: checkpoint activation by helicase and polymerase uncoupling. Genes Dev. 2005 May 5/1/2005; 19(9): 1007-12. PMID: 15879550, PMCID: PMC1360198, PII: 19/9/1007, DOI: 10.1101/gad.1316905, ISSN: 0890-9369.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/15879550.
  • Ball HL, Myers JS, Cortez D. ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation. Mol. Biol. Cell [print-electronic]. 2005 May; 16(5): 2372-81. PMID: 15743907, PMCID: PMC1087242, PII: E04-11-1006, DOI: 10.1091/mbc.E04-11-1006, ISSN: 1059-1524.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/15743907.
  • Cortez D, Glick G, Elledge SJ. Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2004 Jul 7/6/2004; 101(27): 10078-83. PMID: 15210935, PMCID: PMC454167, PII: 0403410101, DOI: 10.1073/pnas.0403410101, ISSN: 0027-8424.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/15210935.
  • Cortez D. Caffeine inhibits checkpoint responses without inhibiting the ataxia-telangiectasia-mutated (ATM) and ATM- and Rad3-related (ATR) protein kinases. J. Biol. Chem [print-electronic]. 2003 Sep 9/26/2003; 278(39): 37139-45. PMID: 12847089, PII: M307088200, DOI: 10.1074/jbc.M307088200, ISSN: 0021-9258.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/12847089.
  • Zou L, Cortez D, Elledge SJ. Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin. Genes Dev. 2002 Jan 1/15/2002; 16(2): 198-208. PMID: 11799063, PMCID: PMC155323, DOI: 10.1101/gad.950302, ISSN: 0890-9369.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/11799063.
  • Cortez D, Guntuku S, Qin J, Elledge SJ. ATR and ATRIP: partners in checkpoint signaling. Science. 2001 Nov 11/23/2001; 294(5547): 1713-6. PMID: 11721054, PII: 294/5547/1713, DOI: 10.1126/science.1065521, ISSN: 0036-8075.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/11721054.
  • Paull T.T., Cortez D., Bowers B., Elledge S.J., Gellert M. Direct DNA binding by Brca1. Proc. Natl. Acad. Sci. USA. 2001; 98: 6086-91.
  • Liu Q, Guntuku S, Cui XS, Matsuoka S, Cortez D, Tamai K, Luo G, Carattini-Rivera S, DeMayo F, Bradley A, Donehower LA, Elledge SJ. Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint. Genes Dev. 2000 Jun 6/15/2000; 14(12): 1448-59. PMID: 10859164, PMCID: PMC316686, ISSN: 0890-9369.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/10859164.
  • Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 2000 Apr 4/15/2000; 14(8): 927-39. PMID: 10783165, PMCID: PMC316544, ISSN: 0890-9369.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/10783165.
  • Cortez D, Elledge SJ. Conducting the mitotic symphony. Nature. 2000; 406: 354-6.
  • Tibbetts, R.S., Cortez, D., Brumbaugh, K.M., Scully, R., Livingston, D., Elledge, S.J., Abraham, R.T. Functional interactions betweeen BRCA1 and the checkpoint kinase ATR during genotoxic stress. Genes and Development. 2000; 14: 2989-3002.
  • Cortez D, Wang Y, Qin J, Elledge SJ. Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science. 1999 Nov 11/5/1999; 286(5442): 1162-6. PMID: 10550055, PII: 7959, ISSN: 0036-8075.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/10550055.
  • Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ. The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Curr. Biol. 1998 Dec 12/3/1998; 8(24): 1300-9. PMID: 9843682, PII: S0960-9822(07)00560-X, ISSN: 0960-9822.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/9843682.
  • Dai, Z., Quackenbush, R.C., Courtney, K.D. Grove, M., Cortez, D., Reuther, G.W., Pendergast, A.M. Oncogenic Abl and Src tyrosine kinases elicit the ubiquitin-dependent degradation of target proteins through a Ras-independent pathway. Genes and Development. 1998; 12: 1415-24.
  • Reuther, J., Reuther, G., Cortez, D., Pendergast, A.M., Baldwin, S.A. Activation of NF-kB is required for Bcr-Abl-mediated tumorigenesis. Genes and Development. 1998; 12: 968-81.
  • Cortez, D., Reuther, G., and Pendergast, A.M. The Bcr-Abl tyrosine kinase activates mitogenic signaling pathways and stimulates G1-to-S phase transition in hematopoietic cells. Oncogene. 1997; 15: 2333-42.
  • Cortez, D., Stoica, G., Pierce, J.H., Pendergast, A.M. The BCR-ABL tyrosine kinase inhibits apoptosis by activating a Ras-dependent signaling pathway. Oncogene. 1996; 13: 2589-94.
  • Cortez, D., Kadlec, L., Pendergast, A.M. Structural and signaling requirements for BCR-ABL-mediated transformation and inhibition of apoptosis. Mol. Cell. Biol. 1995; 15: 5531-41.
  • Ghizisky, M.L., Cortez, D., Pendergast, A.M. Grb2 mutant proteins reverse BCR-ABL-induced transformation. Proc. Natl. Acad. Sci. USA. 1995; 92: 10889-93.
  • Seo KY, Riley J, Cortez D, Wagner ED, Plewa MJ. Characterization of stable high molecular weight mutagenic product(s) of plant-activated m-phenylenediamine. Mutat. Res. 1993 Apr; 299(2): 111-20. PMID: 7680425, PII: 0165-1218(93)90088-U, ISSN: 0027-5107.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/7680425.