David E. Ong, Ph.D.

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1-877-936-8422

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Professor
VICC Member
Researcher

Contact Information:

Vanderbilt University Medical Center
610 Robinson Research Building
Nashville, TN 37232-0146
615-322-4343

Research Specialty

Vitamin A (Retinoid) binding proteins; Retinoid metabolism and function; Retinoid regulated gene expression.

Research Description

Vitamin A is an essential micronutrient that provides not only the signaling molecule essential for vision (11-cis-retinal) but also other retinoids. For example, retinoic acid activates a family of nuclear proteins (the Retinoic Acid Receptors a, b, and g) that direct transcription of genes important for morphological development, proper cellular differentiation and growth control. One member of the RAR group is found in virtually every cell type examined, indicating the essential role that retinoids play in proper bodily function. Remarkably, we still know little about how, where, and when the retinoic acid that activates these receptors is produced. This is currently the major interest of this laboratory. Central to retinoic acid production are a family of carrier proteins, found within certain cells (the cellular retinoid binding proteins), and the plasma carrier protein for the parent retinoid, retinol (retinol binding protein). We have demonstrated that these carrier proteins are directly recognized by other cellular components (enzymes and membrane transporters) to allow the proper delivery and metabolism of retinoids. Our work focuses on defining these interactions at the molecular level as well as using such interactions to find previously unknown enzymes essential for the functions of vitamin A. Techniques used range from site-directed mutagenesis of these proteins to study their interaction with both ligand and enzymes, classical work in enzymology, molecular biological approaches for the study of regulation, as well as immunolocalization at both the light and electron microscopic level of these players to define sites of retinoid action.

Publications

Everts, HB, Sundberg, JP, Ong, DE Immunolocalization of retinoic acid biosynthesis systems in selected sites in rat. Exp Cell Res, 308(2), 309-19, 2005.
Swift, LL, Kakkad, B, Boone, C, Jovanovska, A, Jerome, WG, Mohler, PJ, Ong, DE Microsomal triglyceride transfer protein expression in adipocytes: a new component in fat metabolism. FEBS Lett, 579(14), 3183-9, 2005.
Swift, LL, Jovanovska, A, Kakkad, B, Ong, DE Microsomal triglyceride transfer protein expression in mouse intestine. Histochem Cell Biol, 123(4-5), 475-82, 2005.
Li, X-H, Kakkad, B. and Ong, DE "Estrogen Directly Induces Expression of Retinoic Acid Biosynthetic Enzymes, Compartmentalized Between the Epithelium and Underlying Stromal Cells in Rat Uterus.." Endocrinology, 1454756-4762, 2004.
Li, XH, Kakkad, B, Ong, DE Estrogen directly induces expression of retinoic acid biosynthetic enzymes, compartmentalized between the epithelium and underlying stromal cells in rat uterus. Endocrinology, 145(10), 4756-62, 2004.
Everts, H.B., King Jr., L.E., Sundberg, J.P. and Ong, D.E. "Hair Cycle specific Immunolocalization of Retinoic Acid-synthesizing Enzymes Aldh1a2 and Aldh1a3 Indicate Complex Regulation.." J. Invest. Derm., 123258-263, 2004.
Everts, HB, King, LE, Sundberg, JP, Ong, DE Hair cycle-specific immunolocalization of retinoic acid synthesizing enzymes Aldh1a2 and Aldh1a3 indicate complex regulation. J Invest Dermatol, 123(2), 258-63, 2004.
Li, Xiao-Hong, Ong, David E Cellular retinoic acid-binding protein II gene expression is directly induced by estrogen, but not retinoic acid, in rat uterus. J Biol Chem, 37835819-35825, 2003.
FouchÃ©court, Sophie, Lareyre, Jean-Jacques, Chaurand, Pierre, DaGue, Beverly B, Suzuki, Kichiya, Ong, David E, Olson, Gary E, Matusik, Robert J, Caprioli, Richard M, Orgebin-Crist, Marie-Claire Identification, immunolocalization, regulation, and postnatal development of the lipocalin EP17 (epididymal protein of 17 kilodaltons) in the mouse and rat epididymis. Endocrinology, 144887-900, 2003.
Lucey, Edgar C, Goldstein, Ronald H, Breuer, Raphael, Rexer, Brent N, Ong, David E, Snider, Gordon L Retinoic acid does not affect alveolar septation in adult FVB mice with elastase-induced emphysema. Respiration, 70200-5, 2003.
Swift, Larry L., Zhu, Mei-Ying, Kakkad, Bharati, Jovanovska, Aneta, Neely, M. Diana, Valyi-Nagy, Klara, Roberts, Richard L., Ong, David E., Jerome, W. Gray Subcellular localization of microsomal triglyceride transfer protein (MTP). J Lipid Res, 441841-1849, 2003.
Rexer, Brent N, Ong, David E A novel short-chain alcohol dehydrogenase from rats with retinol dehydrogenase activity, cyclically expressed in uterine epithelium. Biol Reprod, 671555-64, 2002.
Lareyre, J J, Winfrey, V P, Kasper, S, Ong, D E, Matusik, R J, Olson, G E, Orgebin-Crist, M C Gene duplication gives rise to a new 17-kilodalton lipocalin that shows epididymal region-specific expression and testicular factor(s) regulation. Endocrinology, 142(3), 1296-308, 2001.
Rexer, B N, Zheng, W L, Ong, D E Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase 6, absent in MCF-7 cells. Cancer Res, 61(19), 7065-70, 2001.
Mira-y-Lopez, R., Zheng, W.L., Kuppumbatti, Y.S., Rexer, B., Jing, Y. and Ong, D.E "Retinol Conversion To Retinoic Acid Is Impaired In Breast Cancer Cell Lines." J. Cell Physiol., 185302-309 , 2000.
Ruff, S.J. and Ong, D.E "Cellular Retinoic Acid Binding Protein is Associated with Mitochondria." FEBS Lett, 487282-286, 2000.
Ruff, S J, Ong, D E Cellular retinoic acid binding protein is associated with mitochondria. FEBS Lett, 487(2), 282-6, 2000.
Ong, D E, Newcomer, M E, Lareyre, J J, Orgebin-Crist, M C Epididymal retinoic acid-binding protein. Biochim Biophys Acta, 1482(1-2), 209-17, 2000.
Newcomer, M E, Ong, D E Plasma retinol binding protein: structure and function of the prototypic lipocalin. Biochim Biophys Acta, 1482(1-2), 57-64, 2000.
Zheng, W L, Sierra-Rivera, E, Luan, J, Osteen, K G, Ong, D E Retinoic acid synthesis and expression of cellular retinol-binding protein and cellular retinoic acid-binding protein type II are concurrent with decidualization of rat uterine stromal cells. Endocrinology, 141(2), 802-8, 2000.
Mira-Y-Lopez, R, Zheng, W L, Kuppumbatti, Y S, Rexer, B, Jing, Y, Ong, D E Retinol conversion to retinoic acid is impaired in breast cancer cell lines relative to normal cells. J Cell Physiol, 185(2), 302-9, 2000.
Zheng, W.L., Bucco, R.A., Sierra-Rivera, E., Osteen, K.G., Melner, M.H., and Ong, D.E "Synthesis of retinoic acid by CRABP(II) expressing cells of rat ovary." Biol. Reprod., 60110-114, 1999.
Layre, J-J., Thomas, T.Z., Zheng, W.L., Kasper, S., Ong, D.E., Matusik, R.J., and Orgebin-Crist, M-C "A 5-kb promoter fragment of the murine epididymal retinoic acid-binding protein (mE-RABP) drives the tissue-specific, cell-specific, and androgen-regulated expression of a foreign gene in the epididymis of transgenic mice." J. Biol. Chem., 2748282-8290, 1999.
Lareyre, J J, Thomas, T Z, Zheng, W L, Kasper, S, Ong, D E, Orgebin-Crist, M C, Matusik, R J A 5-kilobase pair promoter fragment of the murine epididymal retinoic acid-binding protein gene drives the tissue-specific, cell-specific, and androgen-regulated expression of a foreign gene in the epididymis of transgenic mice. J Biol Chem, 274(12), 8282-90, 1999.
Zheng, W L, Bucco, R A, Sierra-Rievera, E, Osteen, K G, Melner, M H, Ong, D E Synthesis of retinoic acid by rat ovarian cells that express cellular retinoic acid-binding protein-II. Biol Reprod, 60(1), 110-4, 1999.
TrÃ¸en, G, Eskild, W, Fromm, S H, De Luca, L M, Ong, D E, Wardlaw, S A, Reppe, S, Blomhoff, R Vitamin A-sensitive tissues in transgenic mice expressing high levels of human cellular retinol-binding protein type I are not altered phenotypically. J Nutr, 129(9), 1621-7, 1999.
Kingma, P.B., Bok, D. and Ong, D.E "Bovine epidermal fatty acid-binding protein: determination of ligand specificity and cellular localization in retina and testis." Biochemistry, 323250-3257, 1998.
Kingma, P B, Bok, D, Ong, D E Bovine epidermal fatty acid-binding protein: determination of ligand specificity and cellular localization in retina and testis. Biochemistry, 37(10), 3250-7, 1998.
Jamison, R S, Newcomer, M E, Ong, D E Detection of conformational changes in cellular retinoid-binding proteins by limited proteolysis. Methods Mol Biol, 89165-76, 1998.
Layre, J-J., Mattei, M.-G., Kasper, S., Ong, D.E., Matusik, R.J., and Orgebin-Crist, M-C "Genomic organization and chromosomal localization of the murine epididymal retinoic acid-binding protein (mE-RABP) Gene." Molec. Reprod. Develop, 50387-395, 1998.
Lareyre, J J, MattÃ©i, M G, Kasper, S, Ong, D E, Matusik, R J, Orgebin-Crist, M C Genomic organization and chromosomal localization of the murine epididymal retinoic acid-binding protein (mE-RABP) gene. Mol Reprod Dev, 50(4), 387-95, 1998.
Layre, J-J., Zheng, W.L., Zhao, G-Q, Kasper, S., Newcomer, M.E., Matusik, R.J., Ong, D.E., and Orgebin-Crist, M-C "Molecular cloning and hormonal regulation of a murine epididymal retinoic acid-binding protein mRNA." Endocrinology, 1392971-2981, 1998.
Lareyre, J J, Zheng, W L, Zhao, G Q, Kasper, S, Newcomer, M E, Matusik, R J, Ong, D E, Orgebin-Crist, M C Molecular cloning and hormonal regulation of a murine epididymal retinoic acid-binding protein messenger ribonucleic acid. Endocrinology, 139(6), 2971-81, 1998.
Yamamoto, M., Dr?ger, U.C., Ong, D.E., and McCaffery, P "Retinoid binding proteins in the cerebellum and choroid plexus and their relationship to regionalized retinoic acid synthesis and degradation." Euro. J. Biochem, 257344-350, 1998.
Yamamoto, M, DrÃ¤ger, U C, Ong, D E, McCaffery, P Retinoid-binding proteins in the cerebellum and choroid plexus and their relationship to regionalized retinoic acid synthesis and degradation. Eur J Biochem, 257(2), 344-50, 1998.
Zheng, W. L. and Ong, D. E "Spatial and temporal pattern of expression of CRBP and CRABPs in rat uterus during early pregnancy." Biol. Reprod, 58963-970, 1998.
Zheng, W L, Ong, D E Spatial and temporal patterns of expression of cellular retinol-binding protein and cellular retinoic acid-binding proteins in rat uterus during early pregnancy. Biol Reprod, 58(4), 963-70, 1998.
Newcomer, M E, Jamison, R S, Ong, D E Structure and function of retinoid-binding proteins. Subcell Biochem, 3053-80, 1998.
Lareyre, J J, MattÃ©i, M G, Kasper, S, Newcomer, M E, Ong, D E, Matusik, R J, Orgebin-Crist, M C Structure and putative function of a murine epididymal retinoic acid-binding protein (mE-RABP). J Reprod Fertil Suppl, 5359-65, 1998.
Ward, S. J., Chambon, P., Ong, D. E., and B'vik, C "A retinol-binding protein receptor mediated mechanism for embryonic uptake of Vitamin A." Biol. Reprod, 57751-755, 1997.
Ward, S J, Chambon, P, Ong, D E, BÃ¥vik, C A retinol-binding protein receptor-mediated mechanism for uptake of vitamin A to postimplantation rat embryos. Biol Reprod, 57(4), 751-5, 1997.
Dew, S. E. and Ong, D. E "Absorption of retinol from the retinol:retinol binding protein complex by small intestinal gut sheets from the rat." Arch. Biochem. Biophys, 338233-236, 1997.
Dew, S E, Ong, D E Absorption of retinol from the retinol:retinol-binding protein complex by small intestinal gut sheets from the rat. Arch Biochem Biophys, 338(2), 233-6, 1997.
Bucco, R.A., Zheng, W.L., Davis, J.T., Sierra-Rivera, E., Osteen, K.G., Chaudhary, A.K., and Ong, D.E "Cellular retinoic acid-binding protein(II) presence in rat uterine epithelial cells correlates with their synthesis of retinoic acid." Biochemistry, 364009-4014, 1997.
Bucco, R A, Zheng, W L, Davis, J T, Sierra-Rivera, E, Osteen, K G, Chaudhary, A K, Ong, D E Cellular retinoic acid-binding protein(II) presence in rat uterine epithelial cells correlates with their synthesis of retinoic acid. Biochemistry, 36(13), 4009-14, 1997.
B?vik, C., Ward, S. J. and Ong, D. E "Identification of a mechanism to localize generation of retinoic acid in rat embryos." Mechanisms Of Development, 69155-167, 1997.
BÃ¥vik, C, Ward, S J, Ong, D E Identification of a mechanism to localize generation of retinoic acid in rat embryos. Mech Dev, 69(1-2), 155-67, 1997.
Wardlaw, S A, Bucco, R A, Zheng, W L, Ong, D E Variable expression of cellular retinol- and cellular retinoic acid-binding proteins in the rat uterus and ovary during the estrous cycle. Biol Reprod, 56(1), 125-32, 1997.
Wardlaw, S.A., Bucco, R.A., Zheng, W.L., and Ong, D.E "Variable expression of cellular retinol- and cellular retinoic acid-binding proteins in the rat uterus and ovary during the estrous cycle." Biol. Reprod, 56125-132, 1997.
Bucco, R.A., Zheng, W.L., Wardlaw, S.A., Davis, J.T., Sierra-Rivera, E., Osteen, K., Melner, M.H., Kakkad, B. and Ong, D.E "Regulation and localization of cellular retinol-binding protein, retinol-binding protein, cellular retinoic acid-binding protein (CRABP), and CRABP(II) in the uterus of the pseudopregnant rat." Endocrinology, 1373111-3122, 1996.
Zheng, W.L., Bucco, R.A., Schmitt, M. C., Wardlaw, S.A., and Ong, D.E "Localization of CRABP(II) and CRABP in developing rat testis." Endocrinology, 1375028-5035, 1996.
Zheng, W L, Bucco, R A, Schmitt, M C, Wardlaw, S A, Ong, D E Localization of cellular retinoic acid-binding protein (CRABP) II and CRABP in developing rat testis. Endocrinology, 137(11), 5028-35, 1996.
Bucco, R A, Zheng, W L, Wardlaw, S A, Davis, J T, Sierra-Rivera, E, Osteen, K G, Melner, M H, Kakkad, B P, Ong, D E Regulation and localization of cellular retinol-binding protein, retinol-binding protein, cellular retinoic acid-binding protein (CRABP), and CRABP II in the uterus of the pseudopregnant rat. Endocrinology, 137(7), 3111-22, 1996.
Bucco, R.A., Melner, M.H., Gordon, D.S., Leers-Sucheta, S. and Ong, D.E "Inducible expression of cellular retinoic acid-binding protein II in rat ovary: gonadotropin regulation during luteal development." Endocrinology, 1362730-2740, 1995.
Bucco, R A, Melner, M H, Gordon, D S, Leers-Sucheta, S, Ong, D E Inducible expression of cellular retinoic acid-binding protein II in rat ovary: gonadotropin regulation during luteal development. Endocrinology, 136(6), 2730-40, 1995.
Davis, J T, Ong, D E Retinol processing by the peritubular cell from rat testis. Biol Reprod, 52(2), 356-64, 1995.
Davis, J.T. and Ong, D.E "Retinol processing by the peritubular cell from rat testis." Biol. Reprod., 52356-364, 1995.
Jamison, R.S., Kakkad, B., Ebert, D.H., Newcomer, M.E., and Ong, D.E "Test of the contribution of an amino-aromatic hydrogen bond to protein function." Biochemistry, 3411128-11132, 1995.
Jamison, R S, Kakkad, B, Ebert, D H, Newcomer, M E, Ong, D E Test of the contribution of an amino-aromatic hydrogen bond to protein function. Biochemistry, 34(35), 11128-32, 1995.
Schmitt, M C, Jamison, R S, Orgebin-Crist, M C, Ong, D E A novel, testis-specific member of the cellular lipophilic transport protein superfamily, deduced from a complimentary deoxyribonucleic acid clone. Biol Reprod, 51(2), 239-45, 1994.
Jamison, R S, Newcomer, M E, Ong, D E Cellular retinoid-binding proteins: limited proteolysis reveals a conformational change upon ligand binding. Biochemistry, 33(10), 2873-9, 1994.
Ong, D E Cellular transport and metabolism of vitamin A: roles of the cellular retinoid-binding proteins. Nutr Rev, 52(2 Pt 2), S24-31, 1994.
Rigtrup, K M, Kakkad, B, Ong, D E Purification and partial characterization of a retinyl ester hydrolase from the brush border of rat small intestine mucosa: probable identity with brush border phospholipase B. Biochemistry, 33(9), 2661-6, 1994.
Rigtrup, K M, McEwen, L R, Said, H M, Ong, D E Retinyl ester hydrolytic activity associated with human intestinal brush border membranes. Am J Clin Nutr, 60(1), 111-6, 1994.
Dew, S E, Ong, D E Specificity of the retinol transporter of the rat small intestine brush border. Biochemistry, 33(40), 12340-5, 1994.
Ong, D E, Davis, J T, O'Day, W T, Bok, D Synthesis and secretion of retinol-binding protein and transthyretin by cultured retinal pigment epithelium. Biochemistry, 33(7), 1835-42, 1994.
Dew, S E, Wardlaw, S A, Ong, D E Effects of pharmacological retinoids on several vitamin A-metabolizing enzymes. Cancer Res, 53(13), 2965-9, 1993.
Pappas, R S, Newcomer, M E, Ong, D E Endogenous retinoids in rat epididymal tissue and rat and human spermatozoa. Biol Reprod, 48(2), 235-47, 1993.
Schmitt, M C, Ong, D E Expression of cellular retinol-binding protein and lecithin-retinol acyltransferase in developing rat testis. Biol Reprod, 49(5), 972-9, 1993.
Herr, F M, Wardlaw, S A, Kakkad, B, Albrecht, A, Quick, T C, Ong, D E Intestinal vitamin A metabolism: coordinate distribution of enzymes and CRBP(II). J Lipid Res, 34(9), 1545-54, 1993.
Ong, D E Retinoid metabolism during intestinal absorption. J Nutr, 123(2 Suppl), 351-5, 1993.
Newcomer, M E, Pappas, R S, Ong, D E X-ray crystallographic identification of a protein-binding site for both all-trans- and 9-cis-retinoic acid. Proc Natl Acad Sci U S A, 90(19), 9223-7, 1993.
Rigtrup, K M, Ong, D E A retinyl ester hydrolase activity intrinsic to the brush border membrane of rat small intestine. Biochemistry, 31(11), 2920-6, 1992.
Herr, F M, Ong, D E Differential interaction of lecithin-retinol acyltransferase with cellular retinol binding proteins. Biochemistry, 31(29), 6748-55, 1992.
Inagami, S, Ong, D E Purification and partial characterization of cellular retinol-binding protein, type two, from human small intestine. J Nutr, 122(3), 450-6, 1992.
Davis, J T, Ong, D E Synthesis and secretion of retinol-binding protein by cultured rat Sertoli cells. Biol Reprod, 47(4), 528-33, 1992.
Rankin, T L, Ong, D E, Orgebin-Crist, M C The 18-kDa mouse epididymal protein (MEP 10) binds retinoic acid. Biol Reprod, 46(5), 767-71, 1992.
Ong, D E, Lucas, P C, Kakkad, B, Quick, T C Ontogeny of two vitamin A-metabolizing enzymes and two retinol-binding proteins present in the small intestine of the rat. J Lipid Res, 32(9), 1521-7, 1991.
MacDonald, P N, Ong, D E Assay of lecithin-retinol acyltransferase. Methods Enzymol, 189450-9, 1990.
MacDonald, P N, Bok, D, Ong, D E Localization of cellular retinol-binding protein and retinol-binding protein in cells comprising the blood-brain barrier of rat and human. Proc Natl Acad Sci U S A, 87(11), 4265-9, 1990.
Newcomer, M E, Ong, D E Purification and crystallization of a retinoic acid-binding protein from rat epididymis. Identity with the major androgen-dependent epididymal proteins. J Biol Chem, 265(22), 12876-9, 1990.
Finlay, J A, Strom, M, Ong, D E, DeLuca, H F Regulation of cellular retinol binding protein type II by 1,25-dihydroxyvitamin D3. Biochemistry, 29(20), 4914-21, 1990.
Maden, M, Ong, D E, Chytil, F Retinoid-binding protein distribution in the developing mammalian nervous system. Development, 109(1), 75-80, 1990.
Quick, T C, Ong, D E Vitamin A metabolism in the human intestinal Caco-2 cell line. Biochemistry, 29(50), 11116-23, 1990.
Rubin, D C, Ong, D E, Gordon, J I Cellular differentiation in the emerging fetal rat small intestinal epithelium: mosaic patterns of gene expression. Proc Natl Acad Sci U S A, 86(4), 1278-82, 1989.
Maden, M, Ong, D E, Summerbell, D, Chytil, F, Hirst, E A Cellular retinoic acid-binding protein and the role of retinoic acid in the development of the chick embryo. Dev Biol, 135(1), 124-32, 1989.
Shingleton, J L, Skinner, M K, Ong, D E Characteristics of retinol accumulation from serum retinol-binding protein by cultured Sertoli cells. Biochemistry, 28(25), 9641-7, 1989.
Said, H M, Ong, D E, Shingleton, J L Intestinal uptake of retinol: enhancement by bovine milk beta-lactoglobulin. Am J Clin Nutr, 49(4), 690-4, 1989.
Quick, T C, Ong, D E Levels of cellular retinol-binding proteins in the small intestine of rats during pregnancy and lactation. J Lipid Res, 30(7), 1049-54, 1989.
Schaefer, W H, Kakkad, B, Crow, J A, Blair, I A, Ong, D E Purification, primary structure characterization, and cellular distribution of two forms of cellular retinol-binding protein, type II from adult rat small intestine. J Biol Chem, 264(7), 4212-21, 1989.
Shingleton, J L, Skinner, M K, Ong, D E Retinol esterification in Sertoli cells by lecithin-retinol acyltransferase. Biochemistry, 28(25), 9647-53, 1989.
Maden, M, Ong, D E, Summerbell, D, Chytil, F The role of retinoid-binding proteins in the generation of pattern in the developing limb, the regenerating limb and the nervous system. Development, 107 Suppl109-19, 1989.
MacDonald, P N, Ong, D E A lecithin:retinol acyltransferase activity in human and rat liver. Biochem Biophys Res Commun, 156(1), 157-63, 1988.
Ong, D E, MacDonald, P N, Gubitosi, A M Esterification of retinol in rat liver. Possible participation by cellular retinol-binding protein and cellular retinol-binding protein II. J Biol Chem, 263(12), 5789-96, 1988.
MacDonald, P N, Ong, D E Evidence for a lecithin-retinol acyltransferase activity in the rat small intestine. J Biol Chem, 263(25), 12478-82, 1988.
Ong, D E, Chytil, F Presence of novel retinoic acid-binding proteins in the lumen of rat epididymis. Arch Biochem Biophys, 267(2), 474-8, 1988.
Kakkad, B P, Ong, D E Reduction of retinaldehyde bound to cellular retinol-binding protein (type II) by microsomes from rat small intestine. J Biol Chem, 263(26), 12916-9, 1988.
Maden, M, Ong, D E, Summerbell, D, Chytil, F Spatial distribution of cellular protein binding to retinoic acid in the chick limb bud. Nature, 335(6192), 733-5, 1988.
Ong, D E, Kakkad, B, MacDonald, P N Acyl-CoA-independent esterification of retinol bound to cellular retinol-binding protein (type II) by microsomes from rat small intestine. J Biol Chem, 262(6), 2729-36, 1987.
MacDonald, P N, Ong, D E Binding specificities of cellular retinol-binding protein and cellular retinol-binding protein, type II. J Biol Chem, 262(22), 10550-6, 1987.
Ong, D E Cellular retinoid-binding proteins. Arch Dermatol, 123(12), 1693-1695a, 1987.
Ong, D E, Page, D L Cellular retinol-binding protein (type two) is abundant in human small intestine. J Lipid Res, 28(6), 739-45, 1987.
Ong, D E, Takase, S, Chytil, F Cellular vitamin A-binding proteins in the testis. Ann N Y Acad Sci, 513172-8, 1987.
Li, E, Locke, B, Yang, N C, Ong, D E, Gordon, J I Characterization of rat cellular retinol-binding protein II expressed in Escherichia coli. J Biol Chem, 262(28), 13773-9, 1987.
Levin, M S, Li, E, Ong, D E, Gordon, J I Comparison of the tissue-specific expression and developmental regulation of two closely linked rodent genes encoding cytosolic retinol-binding proteins. J Biol Chem, 262(15), 7118-24, 1987.
Chytil, F, Ong, D E Intracellular vitamin A--binding proteins. Annu Rev Nutr, 7321-35, 1987.
Ong, D E, AmÃ©dÃ©e-Manesme, O Liver levels of vitamin A and cellular retinol-binding protein for patients with biliary atresia. Hepatology, 7(2), 253-6, 1987.
Crow, J A, Ong, D E, Chytil, F Specificity of cellular retinol-binding protein in the transfer of retinol to nuclei and chromatin. Arch Biochem Biophys, 254(1), 372-5, 1987.
Ong, D E, Page, D L Quantitation of cellular retinol-binding protein in human organs. Am J Clin Nutr, 44(3), 425-30, 1986.
Li, E, Demmer, L A, Sweetser, D A, Ong, D E, Gordon, J I Rat cellular retinol-binding protein II: use of a cloned cDNA to define its primary structure, tissue-specific expression, and developmental regulation. Proc Natl Acad Sci U S A, 83(16), 5779-83, 1986.
Takase, S, Ong, D E, Chytil, F Transfer of retinoic acid from its complex with cellular retinoic acid-binding protein to the nucleus. Arch Biochem Biophys, 247(2), 328-34, 1986.
Porter, S B, Ong, D E, Chytil, F Vitamin A status affects chromatin structure. Int J Vitam Nutr Res, 56(1), 11-20, 1986.
Crow, J A, Ong, D E Cell-specific immunohistochemical localization of a cellular retinol-binding protein (type two) in the small intestine of rat. Proc Natl Acad Sci U S A, 82(14), 4707-11, 1985.
Porter, S B, Ong, D E, Chytil, F, Orgebin-Crist, M C Localization of cellular retinol-binding protein and cellular retinoic acid-binding protein in the rat testis and epididymis. J Androl, 6(3), 197-212, 1985.
Liau, G, Ong, D E, Chytil, F Partial characterization of nuclear binding sites for retinol delivered by cellular retinol binding protein. Arch Biochem Biophys, 237(2), 354-60, 1985.
Ong, D E Vitamin A-binding proteins. Nutr Rev, 43(8), 225-32, 1985.
Miller, J H, Gates, R E, Ong, D E, King, L E A miniature molecular-sieving column assay for cytoplasmic vitamin A-binding proteins. Anal Biochem, 139(1), 104-14, 1984.
Ong, D E A novel retinol-binding protein from rat. Purification and partial characterization. J Biol Chem, 259(3), 1476-82, 1984.
Bok, D, Ong, D E, Chytil, F Immunocytochemical localization of cellular retinol binding protein in the rat retina. Invest Ophthalmol Vis Sci, 25(8), 877-83, 1984.
Chytil, F, Ong, D E Cellular retinol- and retinoic acid-binding proteins. Adv Nutr Res, 513-29, 1983.
Porter, S B, Fraker, L D, Chytil, F, Ong, D E Localization of cellular retinol-binding protein in several rat tissues. Proc Natl Acad Sci U S A, 80(21), 6586-90, 1983.
Ong, D E, Chytil, F Vitamin A and cancer. Vitam Horm, 40105-44, 1983.
Ong, D E, Goodwin, W J, Jesse, R H, Griffin, A C Presence of cellular retinol and retinoic acid-binding proteins in epidermoid carcinoma of the oral cavity and oropharynx. Cancer, 49(7), 1409-12, 1982.
Ong, D E Purification and partial characterization of cellular retinol-binding protein from human liver. Cancer Res, 42(3), 1033-7, 1982.
Ong, D E, Crow, J A, Chytil, F Radioimmunochemical determination of cellular retinol- and cellular retinoic acid-binding proteins in cytosols of rat tissues. J Biol Chem, 257(22), 13385-9, 1982.
Kylberg, H K, Ong, D E, Chytil, F Cellular retinol binding protein during postanal development of the rat small intestine. Biol Neonate, 39(1-2), 100-4, 1981.
Ong, D E, Chytil, F Immunochemical studies on cellular vitamin A-binding proteins. Ann N Y Acad Sci, 359415-7, 1981.
Liau, G, Ong, D E, Chytil, F Interaction of the retinol/cellular retinol-binding protein complex with isolated nuclei and nuclear components. J Cell Biol, 91(1), 63-8, 1981.
Lotan, R, Ong, D E, Chytil, F Comparison of the level of cellular retinoid-binding proteins and susceptibility to retinoid-induced growth inhibition of various neoplastic cell lines. J Natl Cancer Inst, 64(5), 1259-62, 1980.
Chytil, F, Ong, D E Cellular retinol- and retinoic acid-binding proteins in vitamin A action. Fed Proc, 38(11), 2510-4, 1979.
Takase, S, Ong, D E, Chytil, F Cellular retinol-binding protein allows specific interaction of retinol with the nucleus in vitro. Proc Natl Acad Sci U S A, 76(5), 2204-8, 1979.
Ong, D E, Chytil, F Immunochemical comparison of vitamin A binding proteins of rat. J Biol Chem, 254(18), 8733-5, 1979.
Ong, D E, Markert, C, Chiu, J F Cellular binding proteins for vitamin A in colorectal adenocarcinoma of rat. Cancer Res, 38(12), 4422-6, 1978.
Ong, D E, Chytil, F Cellular retinoic acid-binding protein from rat testis. Purification and characterization. J Biol Chem, 253(13), 4551-4, 1978.
Ong, D E, Chytil, F Cellular retinol-binding protein from rat liver. Purification and characterization. J Biol Chem, 253(3), 828-32, 1978.
Chytil, F, Ong, D E Cellular vitamin A binding proteins. Vitam Horm, 361-32, 1978.
Ong, D E, Tsai, C H, Chytil, F Cellular retinol-binding protein and retinoic acid-binding protein in rat testes: effect of retinol depletion. J Nutr, 106(2), 204-11, 1976.
Ong, D E, Chytil, F Changes in levels of cellular retinol- and retinoic-acid-binding proteins of liver and lung during perinatal development of rat. Proc Natl Acad Sci U S A, 73(11), 3976-8, 1976.
Chytil, F, Ong, D E Mediation of retinoic acid-induced growth and anti-tumour activity. Nature, 260(5546), 49-51, 1976.
Ong, D E, Chytil, F Presence of cellular rentinol and retinoic acid binding proteins in experimental rumors. Cancer Lett, 2(1), 25-30, 1976.
Chytil, F, Page, D L, Ong, D E Presence of cellular retinol and retinoic acid binding proteins in human uterus. Int J Vitam Nutr Res, 45(3), 293-8, 1975.
Ong, D E, Page, D L, Chytil, F Retinoic acid binding protein: occurrence in human tumors. Science, 190(4209), 60-1, 1975.
Ong, D E, Chytil, F Retinoic acid-binding protein in rat tissue. Partial purification and comparison to rat tissue retinol-binding protein. J Biol Chem, 250(15), 6113-7, 1975.
Ong, D E, Chytil, F Specificity of cellular retinol-binding protein for compounds with vitamin A activity. Nature, 255(5503), 74-5, 1975.
Ong, D E, Brady, R N Isolation of cholinergic receptor protein(s) from Torpedo nobiliana by affinity chromatography. Biochemistry, 13(14), 2822-7, 1974.
Ong, D E, Chytil, F Multiple retinol binding proteins in rabbit lung. Biochem Biophys Res Commun, 59(1), 221-9, 1974.
Ong, D E, Brady, R N In vivo studies on the introduction of the 4-t-double bond of the sphingenine moiety of rat brain ceramides. J Biol Chem, 248(11), 3884-8, 1973.
Ong, D E, Emery, T F Ferrichrome biosynthesis: enzyme catalyzed formation of the hydroxamic acid group. Arch Biochem Biophys, 148(1), 77-83, 1972.
Ong, D E, Brady, R N Synthesis of ceramides using N-hydroxysuccinimide esters. J Lipid Res, 13(6), 819-22, 1972.

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