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Vanderbilt-Ingram Cancer CenterVanderbilt-Ingram Cancer Center


David B. Friedman, Ph.D.

Research Associate Professor of Biochemistry

Contact Information:

Vanderbilt University Medical Center
Nashville, TN 37232

Research Specialty

Proteomics; differential protein expression and protein/peptide mass spectrometry.

Research Description

Proteomics is the study of the dynamic protein expression of a tissue, cell type or organism. As an Associate Director of the Proteomics Laboratory in the Mass Spectrometry Research Center, I direct research and technology that targets differential protein expression upon various experimental treatments/conditions, and subsequent protein identification using mass spectrometry. In addition, the Proteomics Laboratory offers the ability to identify proteins resolved on SDS-PAGE gels or in biochemical fractions, as well as characterize and map sites of post-translational modification using mass spectrometry.

The Proteomics Laboratory offers two main proteomics technology platforms, and also embarks on specialized research problems that often go beyond these commercially-available technologies. The first is sensitive and rapid protein identification technologies using single- and multi-dimensional HPLC peptide separations coupled directly to tandem mass spectrometry. The second technology utilizes established 2D gel technology with newly-available flourscent protein tags. Termed DIGE, this technology allows for multiple samples to be separated together and to be compared to a common standard sample. This allows for quantitative comparisons across a minimal number of 2D gels without interference from gel-to-gel variation. This is coupled with rapid and sensitive protein identification using mass spectrometry.