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Vanderbilt-Ingram Cancer CenterVanderbilt-Ingram Cancer Center

 

John A. Oates, M.D.

Thomas F Frist, Senior Professor of Medicine (Clinical Pharmacology)
Professor of Pharmacology
Chair Emeritus of the Department of Medicine
VICC Member
Researcher

Contact Information:

Vanderbilt University Medical Center
536 Robinson Research Building
Nashville, TN 37232-6602
615-343-4847

Research Specialty

Aspirin as an antiplatelet drug for cardioprotection. New therapies for subarachnoid hemorrhage Lipid modification of Amyloid Beta in Alzheimer's disease Platelets in the pathophysiology and treatment of cardiovascular disease.

Research Description

Our research has focused on the participation of the oxygenated metabolites of arachidonic acid (eicosanoids) in the pathophysiology and treatment of human diseases. One pathway of arachidonic acid oxygenation leads to the formation of prostaglandins and related molecules; the initial step in their formation is catalyzed by one of the two cyclooxygenase enzymes, which are inhibited by aspirin and the nonsteroidal anti-inflammatory drugs. One of these enzymes, cyclooxygenase-2 (COX-2), is an inducible enzyme, the biosynthesis of which is stimulated by growth factors, cytokines, and endotoxin, and is inhibited by glucocorticoids.

We are investigating the mechanisms for interindividual variation in the effects of aspirin as an antiplatelet drug employed as a cardioprotective agent. This research is based on our discovery that the irrreversible inhibition of the platelet cycloxygenase-1 is regulated by the redox status of the enzyme. Also under study are the changes in the platelet proteome associated with loss of aspirin's effect on continued administration.

We have found that the delayed vasospasm that can cause severe neurological deficits in subarachnoid hemorrhage is temporally associated with and hypothetically caused by lipid peroxidation resulting from hemoglobin radicals. Our research has identified agents that can inhibit such hemoprotein lcatalyzed ipid peroxidation in vitro and in vivo. We are now investigating a series of hemprotein reductants to determine the compounds most potent in reducing hemoprotein catalyzed lipid peroxidation with the aim of developing a treatment strategy for not only subarachnoid hemorrhage, but also other diseases in which hemoprotein catalyzed lipid peroxidation contributes to the pathophysiology including rhabdomyolysis, myocardial infarction, sickle cell disease, and malaria.

Work on Alzheimer's disease addresses a highly reactive keto-aldehyde product of the cyclooxygenases called levuglandin. We discovered that levuglandins can greatly accelerate the oligomerization of amyloid beta and that the oligomers so formed are neurotoxic. Moreover, we have developed mass spectrometric methods for measuring protein adducts of the levuglandins, and find that they are increased by 12 fold in the brains of patients who died of Alzheimer's disease. W are now working with scavengers of the levuglandins that prevent their reaction with proteins to determine their effects in transgenic mouse models of Alzheimer's disease. Other work addresses mechanisms for decreasing amyloid beta biosynthesis by enhancing cleavage of its precursor protein by the alpha -secretase enzyme.

Clinical Research Description

The participation of the cyclooxygenase enzyme in evolution of highly malignant cancers. Mechanisms determining resistance to aspirin. Methods for assessing activation of platelets in humans. Translational research in subarachnoid hemorrhage.

Publications