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Rebecca A Ihrie

Rebecca A Ihrie, Ph.D.

Assistant Professor of Cancer Biology
Assistant Professor of Neurological Surgery

Contact Information:

761B Preston Research Building
2220 Pierce Avenue
Nashville, TN 37232
615-936-2951
Fax: 615-936-2911

Profile

My research laboratory is focused on understanding how cells in the healthy and diseased brain control their proliferation and their ability to make the different cell types of the brain. We work with basic researchers, medical researchers, and neurosurgeons to study stem cells in the normal brain and stem-like cells in multiple brain tumor types.

Education
 
  • B.S. Honors Biochemistry, University of Michigan
  • Ph.D. Cancer Biology, Stanford University
  • Postdoctoral Fellow, UC - San Francisco
 
Research Description

I am interested in the signaling pathways regulating proliferation and differentiation in neural stem/progenitor cells and brain tumor cells. My long-term goals are to reveal how the normal proliferation and differentiation of stem cells is controlled in the brain, dissect how perturbations of these pathways results in neurological diseases, and leverage these findings to treat human disease. In my independent lab, I lead a group which includes a senior neurosurgery resident, practicing neuropathologist, and two graduate students. I work collaboratively with neurosurgeons, neurologists, developmental biologists, and cancer systems biology faculty to map cell-to-cell differences in proliferative signaling in neural stem cells, neurodevelopmental disorders, and all grades of brain tumors.

My laboratory uses cytometric approaches to 1) identify proliferative pathways that are differentially active within cell subsets in the stem cell niche of the ventricular-subventricular zone), 2) determine how this pattern of activation is altered in disease states, particularly pediatric neurological disorders and adult high-grade brain tumors, and 3) directly identify subpopulations of progenitor-like brain tumor cells using multiparameter single-cell protein analyses.

Publications
  • Yang YP, Ma H, Starchenko A, Huh WJ, Li W, Hickman FE, Zhang Q, Franklin JL, Mortlock DP, Fuhrmann S, Carter BD, Ihrie RA, Coffey RJ. A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo. Cell Rep. 2017 May 5/9/2017; 19(6): 1257-67. PMID: 28494873, PII: S2211-1247(17)30542-9, DOI: 10.1016/j.celrep.2017.04.048, ISSN: 2211-1247.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/28494873.
  • Leelatian N, Doxie DB, Greenplate AR, Sinnaeve J, Ihrie RA, Irish JM. Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis. Curr Protoc Mol Biol. 2017 Apr 4/3/2017; 118: 25C.1.1-25C.1.23. PMID: 28369679, DOI: 10.1002/cpmb.37, ISSN: 1934-3647.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/28369679.
  • Mistry AM, Dewan MC, White-Dzuro GA, Brinson PR, Weaver KD, Thompson RC, Ihrie RA, Chambless LB. Decreased survival in glioblastomas is specific to contact with the ventricular-subventricular zone, not subgranular zone or corpus callosum. J. Neurooncol [print-electronic]. 2017 Apr; 132(2): 341-9. PMID: 28074322, PII: 10.1007/s11060-017-2374-3, DOI: 10.1007/s11060-017-2374-3, ISSN: 1573-7373.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/28074322.
  • Leelatian N, Doxie DB, Greenplate AR, Mobley BC, Lehman JM, Sinnaeve J, Kauffmann RM, Werkhaven JA, Mistry AM, Weaver KD, Thompson RC, Massion PP, Hooks MA, Kelley MC, Chambless LB, Ihrie RA, Irish JM. Single cell analysis of human tissues and solid tumors with mass cytometry. Cytometry B Clin Cytom [print-electronic]. 2017 Jan; 92(1): 68-78. PMID: 27598832, PMCID: PMC5459378, DOI: 10.1002/cyto.b.21481, ISSN: 1552-4957.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/27598832.
  • Mistry AM, Hale AT, Chambless LB, Weaver KD, Thompson RC, Ihrie RA. Influence of glioblastoma contact with the lateral ventricle on survival: a meta-analysis. J. Neurooncol [print-electronic]. 2017 Jan; 131(1): 125-33. PMID: 27644688, PMCID: PMC5262526, PII: 10.1007/s11060-016-2278-7, DOI: 10.1007/s11060-016-2278-7, ISSN: 1573-7373.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/27644688.
  • Rushing G, Ihrie RA. Neural stem cell heterogeneity through time and space in the ventricular-subventricular zone. Front Biol (Beijing) [print-electronic]. 2016 Aug; 11(4): 261-84. PMID: 28367160, PMCID: PMC5371406, DOI: 10.1007/s11515-016-1407-1, ISSN: 1674-7984.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/28367160.
  • Leelatian N, Ihrie RA. Head of the Class: OLIG2 and Glioblastoma Phenotype. Cancer Cell. 2016 May 5/9/2016; 29(5): 613-5. PMID: 27165737, PII: S1535-6108(16)30165-9, DOI: 10.1016/j.ccell.2016.04.007, ISSN: 1878-3686.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/27165737.
  • Chong KM, Leelatian N, Deguire SM, Brockman AA, Earl D, Ihrie RA, Irish JM, Bachmann BO, Sulikowski GA. The use of fluorescently-tagged apoptolidins in cellular uptake and response studies. J. Antibiot [print-electronic]. 2016 Apr; 69(4): 327-30. PMID: 26956792, PII: ja201622, DOI: 10.1038/ja.2016.22, ISSN: 0021-8820.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/26956792.
  • Tong CK, Fuentealba LC, Shah JK, Lindquist RA, Ihrie RA, Guinto CD, Rodas-Rodriguez JL, Alvarez-Buylla A. A Dorsal SHH-Dependent Domain in the V-SVZ Produces Large Numbers of Oligodendroglial Lineage Cells in the Postnatal Brain. Stem Cell Reports [print-electronic]. 2015 Oct 10/13/2015; 5(4): 461-70. PMID: 26411905, PMCID: PMC4624995, PII: S2213-6711(15)00247-7, DOI: 10.1016/j.stemcr.2015.08.013, ISSN: 2213-6711.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/26411905.
  • Shi SQ, Bichell TJ, Ihrie RA, Johnson CH. Ube3a imprinting impairs circadian robustness in Angelman syndrome models. Curr. Biol [print-electronic]. 2015 Mar 3/2/2015; 25(5): 537-45. PMID: 25660546, PMCID: PMC4348236, PII: S0960-9822(14)01650-9, DOI: 10.1016/j.cub.2014.12.047, ISSN: 1879-0445.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25660546.
  • Chiang C, Ihrie RA. Controlling COR competence: BCL-6 regulates neurogenesis and tumor suppression. Cancer Cell. 2014 Dec 12/8/2014; 26(6): 773-4. PMID: 25490438, PII: S1535-6108(14)00471-1, DOI: 10.1016/j.ccell.2014.11.020, ISSN: 1878-3686.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/25490438.
  • Álvarez-Buylla A, Ihrie RA. Sonic hedgehog signaling in the postnatal brain. Semin. Cell Dev. Biol [print-electronic]. 2014 Sep; 33: 105-11. PMID: 24862855, PMCID: PMC4130786, PII: S1084-9521(14)00145-1, DOI: 10.1016/j.semcdb.2014.05.008, ISSN: 1096-3634.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/24862855.
  • Haskins WE, Zablotsky BL, Foret MR, Ihrie RA, Alvarez-Buylla A, Eisenman RN, Berger MS, Lin CH. Molecular Characteristics in MRI-Classified Group 1 Glioblastoma Multiforme. Front Oncol. 2013; 3: 182. PMID: 23875172, PMCID: PMC3708153, DOI: 10.3389/fonc.2013.00182, ISSN: 2234-943X.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/23875172.
  • Huillard E, Hashizume R, Phillips JJ, Griveau A, Ihrie RA, Aoki Y, Nicolaides T, Perry A, Waldman T, McMahon M, Weiss WA, Petritsch C, James CD, Rowitch DH. Cooperative interactions of BRAFV600E kinase and CDKN2A locus deficiency in pediatric malignant astrocytoma as a basis for rational therapy. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 May 5/29/2012; 109(22): 8710-5. PMID: 22586120, PMCID: PMC3365162, PII: 1117255109, DOI: 10.1073/pnas.1117255109, ISSN: 1091-6490.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/22586120.
  • Sanai N, Nguyen T, Ihrie RA, Mirzadeh Z, Tsai HH, Wong M, Gupta N, Berger MS, Huang E, Garcia-Verdugo JM, Rowitch DH, Alvarez-Buylla A. Corridors of migrating neurons in the human brain and their decline during infancy. Nature. 2011 Oct 10/20/2011; 478(7369): 382-6. PMID: 21964341, PMCID: PMC3197903, PII: nature10487, DOI: 10.1038/nature10487, ISSN: 1476-4687.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21964341.
  • Ihrie RA, Shah JK, Harwell CC, Levine JH, Guinto CD, Lezameta M, Kriegstein AR, Alvarez-Buylla A. Persistent sonic hedgehog signaling in adult brain determines neural stem cell positional identity. Neuron. 2011 Jul 7/28/2011; 71(2): 250-62. PMID: 21791285, PMCID: PMC3346180, PII: S0896-6273(11)00404-1, DOI: 10.1016/j.neuron.2011.05.018, ISSN: 1097-4199.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21791285.
  • Ihrie RA, Alvarez-Buylla A. Lake-front property: a unique germinal niche by the lateral ventricles of the adult brain. Neuron. 2011 May 5/26/2011; 70(4): 674-86. PMID: 21609824, PMCID: PMC3346178, PII: S0896-6273(11)00382-5, DOI: 10.1016/j.neuron.2011.05.004, ISSN: 1097-4199.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21609824.
  • Jheon AH, Mostowfi P, Snead ML, Ihrie RA, Sone E, Pramparo T, Attardi LD, Klein OD. PERP regulates enamel formation via effects on cell-cell adhesion and gene expression. J. Cell. Sci [print-electronic]. 2011 Mar 3/1/2011; 124(Pt 5): 745-54. PMID: 21285247, PMCID: PMC3039019, PII: jcs.078071, DOI: 10.1242/jcs.078071, ISSN: 1477-9137.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/21285247.
  • Beaudry VG, Ihrie RA, Jacobs SB, Nguyen B, Pathak N, Park E, Attardi LD. Loss of the desmosomal component perp impairs wound healing in vivo. Dermatol Res Pract [print-electronic]. 2010; 2010: 759731. PMID: 20628490, PMCID: PMC2902749, DOI: 10.1155/2010/759731, ISSN: 1687-6113.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/20628490.
  • Ihrie RA, Alvarez-Buylla A. Cells in the astroglial lineage are neural stem cells. Cell Tissue Res [print-electronic]. 2008 Jan; 331(1): 179-91. PMID: 17786483, DOI: 10.1007/s00441-007-0461-z, ISSN: 1432-0878.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/17786483.
  • Ihrie RA, Bronson RT, Attardi LD. Adult mice lacking the p53/p63 target gene Perp are not predisposed to spontaneous tumorigenesis but display features of ectodermal dysplasia syndromes [letter]. Cell Death Differ [print-electronic]. 2006 Sep; 13(9): 1614-8. PMID: 16485031, PII: 4401871, DOI: 10.1038/sj.cdd.4401871, ISSN: 1350-9047.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/16485031.
  • Marques MR, Ihrie RA, Horner JS, Attardi LD. The requirement for perp in postnatal viability and epithelial integrity reflects an intrinsic role in stratified epithelia. J. Invest. Dermatol. 2006 Jan; 126(1): 69-73. PMID: 16417219, PMCID: PMC2879258, PII: 5700032, DOI: 10.1038/sj.jid.5700032, ISSN: 0022-202X.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/16417219.
  • Marques MR, Horner JS, Ihrie RA, Bronson RT, Attardi LD. Mice lacking the p53/p63 target gene Perp are resistant to papilloma development. Cancer Res. 2005 Aug 8/1/2005; 65(15): 6551-6. PMID: 16061634, PII: 65/15/6551, DOI: 10.1158/0008-5472.CAN-05-0366, ISSN: 0008-5472.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/16061634.
  • Ihrie RA, Attardi LD. A new Perp in the lineup: linking p63 and desmosomal adhesion. Cell Cycle [print-electronic]. 2005 Jul; 4(7): 873-6. PMID: 15970683, PII: 1836, ISSN: 1551-4005.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/15970683.
  • Ihrie RA, Marques MR, Nguyen BT, Horner JS, Papazoglu C, Bronson RT, Mills AA, Attardi LD. Perp is a p63-regulated gene essential for epithelial integrity. Cell. 2005 Mar 3/25/2005; 120(6): 843-56. PMID: 15797384, PII: S0092-8674(05)00046-2, DOI: 10.1016/j.cell.2005.01.008, ISSN: 0092-8674.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/15797384.
  • Beer S, Zetterberg A, Ihrie RA, McTaggart RA, Yang Q, Bradon N, Arvanitis C, Attardi LD, Feng S, Ruebner B, Cardiff RD, Felsher DW. Developmental context determines latency of MYC-induced tumorigenesis. PLoS Biol [print-electronic]. 2004 Nov; 2(11): e332. PMID: 15455033, PMCID: PMC519000, DOI: 10.1371/journal.pbio.0020332, ISSN: 1545-7885.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/15455033.
  • Ihrie RA, Attardi LD. Perp-etrating p53-dependent apoptosis. Cell Cycle [print-electronic]. 2004 Mar; 3(3): 267-9. PMID: 14726658, PII: 722, ISSN: 1538-4101.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/14726658.
  • Ihrie RA, Reczek E, Horner JS, Khachatrian L, Sage J, Jacks T, Attardi LD. Perp is a mediator of p53-dependent apoptosis in diverse cell types. Curr. Biol. 2003 Nov 11/11/2003; 13(22): 1985-90. PMID: 14614825, PII: S0960982203008236, ISSN: 0960-9822.
    Available from: http://www.ncbi.nlm.nih.gov/pubmed/14614825.