The VICC.ORG Directory of Doctors, Healthcare Providers & Researchers
Richard M. Caprioli, Ph.D.
See also:
Stanford Moore Chair in Biochemistry
Director of the Mass Spectrometry Research Center
VICC Member
Researcher
Contact Information:
Vanderbilt Mass Spectrometry Research Center
465 21st Ave. S., 9160 MRB III
Nashville, TN 37232-8575
615-322-4336
Fax: 615-343-8372
Profile
Dr. Caprioli is the Stanford Moore Chair in Biochemistry and the Director of the Mass Spectrometry Research Center at Vanderbilt University Medical Center.
Research Specialty
Metabolism of cellular peptides
Research Description
The research interests of this laboratory are aimed at the investigation of biological processes involving the synthesis, modification, storage and degradation of certain peptides and proteins using modern mass spectrometric methods of analysis to follow molecular events. In recent years there has been a great amount of interest in investigating the biochemical events involved in the metabolism of peptides, primarily in the brain and gut of mammals, encompassing the enzymatic breakdown of these peptides, their production from peptide and protein precursors, and the disruption of these processes by certain xenobiotics. Modern mass spectrometric techniques are used in these studies, including electrospray and matrix-assisted laser desorption ionization mass spectrometry. This research is generally carried out with two experimental approaches.
The first involves in vivo metabolic studies in live animals (rats) using microdialysis sampling procedures together with molecular identification and quantitation by electrospray tandem mass spectrometry. This experimental approach allows us to measure metabolic events that occur in freely moving animals at a level of 50-100 attomoles in the tissue dialysate. Integrated technology for microdialysis/mass spectrometry utilizes quadrupole mass spectrometry (MS/MS) and micro-electrospray ionization (micro-ESI) for very high sensitivity measurements. The micro-ESI source has been optimized to produce ions from the electrospray process at sub-microliter per min flow rates. The application of combined microdialysis/ESI mass spectrometry is ongoing in four areas, i) Investigation of the kinetics and quantitative aspects of the in vivo metabolism of the neuropeptides and enkephalin neuropeptides, ii) Studies of the kinetics and differential processing of opioid peptide fragment precursors in vivo, iii) Investigation of the in vivo quantitative production and enzymatic degradation of N-acetylaspartyl-glutamate in seizure disorders using a rat model, and iv) Development and measurements of in vivo drug pharmacokinetics, using the drugs haloperidol, valproic acid, cocaine, amphetamine, and the new GABA antagonist n-butyl-3 aminopropylphosphinic acid.
The second experimental approach used in the study of cellular peptide metabolism utilizes new molecular imaging technology that is being developed in our lab. This method involves molecular mapping of animal tissue through the production of ion images obtained from the analysis of mammalian tissue slices by matrix-assisted laser desorption mass spectrometry (MALDI MS)12. This technique permits a tissue section to be mapped in multiple molecular weight values, localizing the molecules in an X, Y coordinate representation of the sample. Ion images are produced by repetitive exposure of the sample to the laser beam, where adjacent spots are irradiated, resulting in an ordered array of mass spectra that are keyed to specific locations in the sample. From one raster of the sample, a specific ion image, at any chosen m/z value, could be produced to give the spatial arrangement of molecules of interest. Current work involves; i) Instrument development involving modifications to an existing commercial MALDI (TOF) mass spectrometer, including changes to the laser optics, target movement system, and instrument control hardware and software, ii) Development of methods for sample preparation and target surface modifications to achieve high sensitivity and high image resolution, with molecular specificity, and iii) Applications to specific research areas involving questions about certain spatial distributions of molecules within specific tissues.
Publications
- Aerni, HR, Cornett, DS, Caprioli, RM High-throughput profiling of formalin-fixed paraffin-embedded tissue using parallel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry. Anal Chem, 81(17), 7490-5, 2009.
- Xu, BJ, Li, J, Beauchamp, RD, Shyr, Y, Li, M, Washington, MK, Yeatman, TJ, Whitehead, RH, Coffey, RJ, Caprioli, RM Identification of early intestinal neoplasia protein biomarkers using laser capture microdissection and MALDI MS. Mol Cell Proteomics, 8(5), 936-45, 2009.
- Konkle, ME, Hargrove, TY, Kleshchenko, YY, von Kries, JP, Ridenour, W, Uddin, MJ, Caprioli, RM, Marnett, LJ, Nes, WD, Villalta, F, Waterman, MR, Lepesheva, GI Indomethacin amides as a novel molecular scaffold for targeting Trypanosoma cruzi sterol 14alpha-demethylase. J Med Chem, 52(9), 2846-53, 2009.
- Grey, AC, Chaurand, P, Caprioli, RM, Schey, KL MALDI imaging mass spectrometry of integral membrane proteins from ocular lens and retinal tissue. J Proteome Res, 8(7), 3278-83, 2009.
- Mathur, BN, Caprioli, RM, Deutch, AY Proteomic analysis illuminates a novel structural definition of the claustrum and insula. Cereb Cortex, 19(10), 2372-9, 2009.
- Sanders, ME, Dias, EC, Xu, BJ, Mobley, JA, Billheimer, D, Roder, H, Grigorieva, J, Dowsett, M, Arteaga, CL, Caprioli, RM Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS. J Proteome Res, 7(4), 1500-7, 2008.
- Seeley, EH, Oppenheimer, SR, Mi, D, Chaurand, P, Caprioli, RM Enhancement of Protein Sensitivity for MALDI Imaging Mass Spectrometry After Chemical Treatment of Tissue Sections. J Am Soc Mass Spectrom, 2008.
- Groseclose, MR, Massion, PP, Chaurand, P, Caprioli, RM High-throughput proteomic analysis of formalin-fixed paraffin-embedded tissue microarrays using MALDI imaging mass spectrometry. Proteomics, 8(18), 3715-24, 2008.
- Manier, ML, Cornett, DS, Hachey, DL, Caprioli, RM Identification of dimethyldioctadecylammonium ion (m/z 550.6) and related species (m/z 522.6, 494.6) as a source of contamination in mass spectrometry. J Am Soc Mass Spectrom, 19(5), 666-70, 2008.
- Chaurand, P, Latham, JC, Lane, KB, Mobley, JA, Polosukhin, VV, Wirth, PS, Nanney, LB, Caprioli, RM Imaging Mass Spectrometry of Intact Proteins from Alcohol-Preserved Tissue Specimens: Bypassing Formalin Fixation. J Proteome Res, 7(8), 3543-3555, 2008.
- Andersson, M, Groseclose, MR, Deutch, AY, Caprioli, RM Imaging mass spectrometry of proteins and peptides: 3D volume reconstruction. Nat Methods, 5(1), 101-8, 2008.
- Burnum, KE, Tranguch, S, Mi, D, Daikoku, T, Dey, SK, Caprioli, RM Imaging mass spectrometry reveals unique protein profiles during embryo implantation. Endocrinology, 149(7), 3274-8, 2008.
- Hardesty, WM, Caprioli, RM In situ molecular imaging of proteins in tissues using mass spectrometry. Anal Bioanal Chem, 391(3), 899-903, 2008.
- Sinha, TK, Khatib-Shahidi, S, Yankeelov, TE, Mapara, K, Ehtesham, M, Cornett, DS, Dawant, BM, Caprioli, RM, Gore, JC Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imaging. Nat Methods, 5(1), 57-9, 2008.
- Cornett, DS, Frappier, SL, Caprioli, RM MALDI-FTICR imaging mass spectrometry of drugs and metabolites in tissue. Anal Chem, 80(14), 5648-53, 2008.
- Corbin, BD, Seeley, EH, Raab, A, Feldmann, J, Miller, MR, Torres, VJ, Anderson, KL, Dattilo, BM, Dunman, PM, Gerads, R, Caprioli, RM, Nacken, W, Chazin, WJ, Skaar, EP Metal chelation and inhibition of bacterial growth in tissue abscesses. Science, 319(5865), 962-5, 2008.
- Seeley, EH, Caprioli, RM Molecular imaging of proteins in tissues by mass spectrometry. Proc Natl Acad Sci U S A, 105(47), 18126-31, 2008.
- Chaurand, P, Rahman, MA, Hunt, T, Mobley, JA, Gu, G, Latham, JC, Caprioli, RM, Kasper, S Monitoring mouse prostate development by profiling and imaging mass spectrometry. Mol Cell Proteomics, 7(2), 411-23, 2008.
- Puolitaival, SM, Burnum, KE, Cornett, DS, Caprioli, RM Solvent-free matrix dry-coating for MALDI imaging of phospholipids. J Am Soc Mass Spectrom, 19(6), 882-6, 2008.
- Lee, YT, Dimitrova, YN, Schneider, G, Ridenour, WB, Bhattacharya, S, Soss, SE, Caprioli, RM, Filipek, A, Chazin, WJ Structure of the S100A6 complex with a fragment from the C-terminal domain of Siah-1 interacting protein: a novel mode for S100 protein target recognition. Biochemistry, 47(41), 10921-32, 2008.
- Caldwell, RL, Opalenik, SR, Davidson, JM, Caprioli, RM, Nanney, LB Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds. Wound Repair Regen, 16(3), 442-9, 2008.
- M''Koma, AE, Blum, DL, Norris, JL, Koyama, T, Billheimer, D, Motley, S, Ghiassi, M, Ferdowsi, N, Bhowmick, I, Chang, SS, Fowke, JH, Caprioli, RM, Bhowmick, NA Detection of pre-neoplastic and neoplastic prostate disease by MALDI profiling of urine. Biochem Biophys Res Commun, 353(3), 829-34, 2007.
- Yildiz, PB, Shyr, Y, Rahman, JS, Wardwell, NR, Zimmerman, LJ, Shakhtour, B, Gray, WH, Chen, S, Li, M, Roder, H, Liebler, DC, Bigbee, WL, Siegfried, JM, Weissfeld, JL, Gonzalez, AL, Ninan, M, Johnson, DH, Carbone, DP, Caprioli, RM, Massion, PP Diagnostic accuracy of MALDI mass spectrometric analysis of unfractionated serum in lung cancer. J Thorac Oncol, 2(10), 893-901, 2007.
- Herring, KD, Oppenheimer, SR, Caprioli, RM Direct tissue analysis by matrix-assisted laser desorption ionization mass spectrometry: application to kidney biology. Semin Nephrol, 27(6), 597-608, 2007.
- Groseclose, MR, Andersson, M, Hardesty, WM, Caprioli, RM Identification of proteins directly from tissue: in situ tryptic digestions coupled with imaging mass spectrometry. J Mass Spectrom, 42(2), 254-62, 2007.
- Chaurand, P, Schriver, KE, Caprioli, RM Instrument design and characterization for high resolution MALDI-MS imaging of tissue sections. J Mass Spectrom, 42(4), 476-89, 2007.
- Cornett, DS, Reyzer, ML, Chaurand, P, Caprioli, RM MALDI imaging mass spectrometry: molecular snapshots of biochemical systems. Nat Methods, 4(10), 828-33, 2007.
- Reyzer, ML, Caprioli, RM MALDI-MS-based imaging of small molecules and proteins in tissues. Curr Opin Chem Biol, 11(1), 29-35, 2007.
- Friedman, DB, Wang, SE, Whitwell, CW, Caprioli, RM, Arteaga, CL Multivariable difference gel electrophoresis and mass spectrometry: a case study on transforming growth factor-beta and ERBB2 signaling. Mol Cell Proteomics, 6(1), 150-69, 2007.
- McLean, JA, Ridenour, WB, Caprioli, RM Profiling and imaging of tissues by imaging ion mobility-mass spectrometry. J Mass Spectrom, 42(8), 1099-105, 2007.
- Caldwell, RL, Opalenik, SR, Davidson, JM, Caprioli, RM, Nanney, LB Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds. Wound Repair Regen, 2007.
- Cornett, DS, Mobley, JA, Dias, EC, Andersson, M, Arteaga, CL, Sanders, ME, Caprioli, RM A novel histology-directed strategy for MALDI-MS tissue profiling that improves throughput and cellular specificity in human breast cancer. Mol Cell Proteomics, 5(10), 1975-83, 2006.
- Aerni, HR, Cornett, DS, Caprioli, RM Automated acoustic matrix deposition for MALDI sample preparation. Anal Chem, 78(3), 827-34, 2006.
- Sköld, K, Svensson, M, Nilsson, A, Zhang, X, Nydahl, K, Caprioli, RM, Svenningsson, P, Andrén, PE Decreased Striatal Levels of PEP-19 Following MPTP Lesion in the Mouse. J Proteome Res, 5(2), 262-269, 2006.
- Khatib-Shahidi, S, Andersson, M, Herman, JL, Gillespie, TA, Caprioli, RM Direct molecular analysis of whole-body animal tissue sections by imaging MALDI mass spectrometry. Anal Chem, 78(18), 6448-56, 2006.
- Suzuki, K, Yu, X, Chaurand, P, Araki, Y, Lareyre, JJ, Caprioli, RM, Matusik, RJ, Orgebin-Crist, MC Epididymis-specific promoter-driven gene targeting: A transcription factor which regulates epididymis-specific gene expression. Mol Cell Endocrinol, 2006.
- Luka, Z, Ham, AJ, Norris, JL, Yeo, EJ, Yermalitsky, V, Glenn, B, Caprioli, RM, Liebler, DC, Wagner, C Identification of phosphorylation sites in glycine N-methyltransferase from rat liver. Protein Sci, 15(4), 785-94, 2006.
- Chaurand, P, Cornett, DS, Caprioli, RM Molecular imaging of thin mammalian tissue sections by mass spectrometry. Curr Opin Biotechnol, 17(4), 431-6, 2006.
- Chaurand, P, Norris, JL, Cornett, DS, Mobley, JA, Caprioli, RM New developments in profiling and imaging of proteins from tissue sections by MALDI mass spectrometry. J Proteome Res, 5(11), 2889-900, 2006.
- Massion, PP, Caprioli, RM Proteomic strategies for the characterization and the early detection of lung cancer. J Thorac Oncol, 1(9), 1027-39, 2006.
- Amann, JM, Chaurand, P, Gonzalez, A, Mobley, JA, Massion, PP, Carbone, DP, Caprioli, RM Selective profiling of proteins in lung cancer cells from fine-needle aspirates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Cancer Res, 12(17), 5142-50, 2006.
- Norris, JL, Porter, NA, Caprioli, RM Combination detergent/MALDI matrix: functional cleavable detergents for mass spectrometry. Anal Chem, 77(15), 5036-40, 2005.
- Caprioli, RM Deciphering protein molecular signatures in cancer tissues to aid in diagnosis, prognosis, and therapy. Cancer Res, 65(23), 10642-5, 2005.
- Laurent, C, Levinson, DF, Schwartz, SA, Harrington, PB, Markey, SP, Caprioli, RM, Levitt, P Direct profiling of the cerebellum by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: A methodological study in postnatal and adult mouse. J Neurosci Res, 81(5), 613-21, 2005.
- Caprioli, R Editorial. J Mass Spectrom, 40(4), 429, 2005.
- Gao, N, Ishii, K, Mirosevich, J, Kuwajima, S, Oppenheimer, SR, Roberts, RL, Jiang, M, Yu, X, Shappell, SB, Caprioli, RM, Stoffel, M, Hayward, SW, Matusik, RJ Forkhead box A1 regulates prostate ductal morphogenesis and promotes epithelial cell maturation. Development, 132(15), 3431-43, 2005.
- Xie, L, Xu, BJ, Gorska, AE, Shyr, Y, Schwartz, SA, Cheng, N, Levy, S, Bierie, B, Caprioli, RM, Moses, HL Genomic and proteomic analysis of mammary tumors arising in transgenic mice. J Proteome Res, 4(6), 2088-98, 2005.
- Zimmerman, LJ, Wernke, GR, Caprioli, RM, Liebler, DC Identification of protein fragments as pattern features in MALDI-MS analyses of serum. J Proteome Res, 4(5), 1672-80, 2005.
- Chaurand, P, Schwartz, SA, Reyzer, ML, Caprioli, RM Imaging mass spectrometry: principles and potentials. Toxicol Pathol, 33(1), 92-101, 2005.
- Reyzer, ML, Caprioli, RM MALDI mass spectrometry for direct tissue analysis: a new tool for biomarker discovery. J Proteome Res, 4(4), 1138-42, 2005.
- Ma, S, Hill, KE, Burk, RF, Caprioli, RM Mass spectrometric determination of selenenylsulfide linkages in rat selenoprotein P. J Mass Spectrom, 40(3), 400-4, 2005.
- Norris, JL, Hangauer, MJ, Porter, NA, Caprioli, RM Nonacid cleavable detergents applied to MALDI mass spectrometry profiling of whole cells. J Mass Spectrom, 40(10), 1319-26, 2005.
- Hu, X, Friedman, D, Hill, S, Caprioli, R, Nicholson, W, Powers, AC, Hunter, L, Limbird, LE Proteomic exploration of pancreatic islets in mice null for the alpha2A adrenergic receptor. J Mol Endocrinol, 35(1), 73-88, 2005.
- Xu, BJ, Shyr, Y, Liang, X, Ma, LJ, Donnert, EM, Roberts, JD, Zhang, X, Kon, V, Brown, NJ, Caprioli, RM, Fogo, AB Proteomic patterns and prediction of glomerulosclerosis and its mechanisms. J Am Soc Nephrol, 16(10), 2967-75, 2005.
- Rahman, SM, Shyr, Y, Yildiz, PB, Gonzalez, AL, Li, H, Zhang, X, Chaurand, P, Yanagisawa, K, Slovis, BS, Miller, RF, Ninan, M, Miller, YE, Franklin, WA, Caprioli, RM, Carbone, DP, Massion, PP Proteomic patterns of preinvasive bronchial lesions. Am J Respir Crit Care Med, 172(12), 1556-62, 2005.
- Schwartz, SA, Weil, RJ, Thompson, RC, Shyr, Y, Moore, JH, Toms, SA, Johnson, MD, Caprioli, RM Proteomic-based prognosis of brain tumor patients using direct-tissue matrix-assisted laser desorption ionization mass spectrometry. Cancer Res, 65(17), 7674-81, 2005.
- Crecelius, AC, Cornett, DS, Caprioli, RM, Williams, B, Dawant, BM, Bodenheimer, B Three-dimensional visualization of protein expression in mouse brain structures using imaging mass spectrometry. J Am Soc Mass Spectrom, 16(7), 1093-9, 2005.
- Caldwell, RL, Caprioli, RM Tissue profiling by mass spectrometry: a review of methodology and applications. Mol Cell Proteomics, 4(4), 394-401, 2005.
- Chaurand, Pierre, Schwartz, Sarah A, Caprioli, Richard M Assessing protein patterns in disease using imaging mass spectrometry. J Proteome Res, 3(2), 245-52, 2004.
- Reyzer, Michelle L, Caldwell, Robert L, Dugger, Teresa C, Forbes, James T, Ritter, Christoph A, Guix, Marta, Arteaga, Carlos L, Caprioli, Richard M Early changes in protein expression detected by mass spectrometry predict tumor response to molecular therapeutics. Cancer Res, 64(24), 9093-100, 2004.
- Chaurand, Pierre, Schwartz, Sarah A, Billheimer, Dean, Xu, Baogang J, Crecelius, Anna, Caprioli, Richard M Integrating histology and imaging mass spectrometry. Anal Chem, 76(4), 1145-55, 2004.
- Schwartz, Sarah A, Weil, Robert J, Johnson, Mahlon D, Toms, Steven A, Caprioli, Richard M Protein profiling in brain tumors using mass spectrometry: feasibility of a new technique for the analysis of protein expression. Clin Cancer Res, 10(3), 981-7, 2004.
- Friedman, David B, Hill, Salisha, Keller, Jeffrey W, Merchant, Nipun B, Levy, Shawn E, Coffey, Robert J, Caprioli, Richard M Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics, 4(3), 793-811, 2004.
- Chaurand, Pierre, Sanders, Melinda E, Jensen, Roy A, Caprioli, Richard M Proteomics in diagnostic pathology: profiling and imaging proteins directly in tissue sections. Am J Pathol, 165(4), 1057-68, 2004.
- Zhen, Y, Caprioli, RM, Staros, JV Characterization of glycosylation sites of the epidermal growth factor receptor. Biochemistry, 42(18), 5478-92, 2003.
- Reyzer, Michelle L, Hsieh, Yunsheng, Ng, Kwokei, Korfmacher, Walter A, Caprioli, Richard M Direct analysis of drug candidates in tissue by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom, 38(10), 1081-92, 2003.
- Schwartz, SA, Reyzer, ML, Caprioli, RM Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. J Mass Spectrom, 38(7), 699-708, 2003.
- Phillip Hochgesang, G, Nemeth-Cawley, JF, Rowlinson, SW, Caprioli, RM, Marnett, LJ Functional analysis of the molecular determinants of cyclooxygenase-2 acetylation by 2-acetoxyphenylhept-2-ynyl sulfide. Arch Biochem Biophys, 409(1), 127-33, 2003.
- Fouchécourt, S, Lareyre, JJ, Chaurand, P, DaGue, BB, Suzuki, K, Ong, DE, Olson, GE, Matusik, RJ, Caprioli, RM, Orgebin-Crist, MC Identification, immunolocalization, regulation, and postnatal development of the lipocalin EP17 (epididymal protein of 17 kilodaltons) in the mouse and rat epididymis. Endocrinology, 144(3), 887-900, 2003.
- Ma, S, Caprioli, RM, Hill, KE, Burk, RF Loss of selenium from selenoproteins: conversion of selenocysteine to dehydroalanine in vitro. J Am Soc Mass Spectrom, 14(6), 593-600, 2003.
- Ma, S, Hill, KE, Burk, RF, Caprioli, RM Mass spectrometric identification of N- and O-glycosylation sites of full-length rat selenoprotein P and determination of selenide-sulfide and disulfide linkages in the shortest isoform. Biochemistry, 42(32), 9703-11, 2003.
- Norris, Jeremy L, Porter, Ned A, Caprioli, Richard M Mass spectrometry of intracellular and membrane proteins using cleavable detergents. Anal Chem, 75(23), 6642-7, 2003.
- Yanagisawa, Kiyoshi, Xu, Baogang J, Carbone, David P, Caprioli, Richard M Molecular fingerprinting in human lung cancer. Clin Lung Cancer, 5(2), 113-8, 2003.
- Chaurand, Pierre, Fouchécourt, Sophie, DaGue, Beverly B, Xu, Baogang J, Reyzer, Michelle L, Orgebin-Crist, Marie-Claire, Caprioli, Richard M Profiling and imaging proteins in the mouse epididymis by imaging mass spectrometry. Proteomics, 3(11), 2221-39, 2003.
- Yanagisawa, Kiyoshi, Shyr, Yu, Xu, Baogang J, Massion, Pierre P, Larsen, Paul H, White, Bill C, Roberts, John R, Edgerton, Mary, Gonzalez, Adriana, Nadaf, Sorena, Moore, Jason H, Caprioli, Richard M, Carbone, David P Proteomic patterns of tumour subsets in non-small-cell lung cancer. Lancet, 362(9382), 433-9, 2003.
- Xu, BJ, Caprioli, RM, Sanders, ME, Jensen, RA Direct analysis of laser capture microdissected cells by MALDI mass spectrometry. J Am Soc Mass Spectrom, 13(11), 1292-7, 2002.
- Chaurand, P, Caprioli, RM Direct profiling and imaging of peptides and proteins from mammalian cells and tissue sections by mass spectrometry. Electrophoresis, 23(18), 3125-35, 2002.
- Fouchécourt, S, Chaurand, P, DaGue, BB, Lareyre, JJ, Matusik, RJ, Caprioli, RM, Orgebin-Crist, MC Epididymal lipocalin-type prostaglandin D2 synthase: identification using mass spectrometry, messenger RNA localization, and immunodetection in mouse, rat, hamster, and monkey. Biol Reprod, 66(2), 524-33, 2002.
- Chaurand, P, Schwartz, SA, Caprioli, RM Imaging mass spectrometry: a new tool to investigate the spatial organization of peptides and proteins in mammalian tissue sections. Curr Opin Chem Biol, 6(5), 676-81, 2002.
- Ma, S, Hill, KE, Caprioli, RM, Burk, RF Mass spectrometric characterization of full-length rat selenoprotein P and three isoforms shortened at the C terminus. Evidence that three UGA codons in the mRNA open reading frame have alternative functions of specifying selenocysteine insertion or translation termination. J Biol Chem, 277(15), 12749-54, 2002.
- Boutaud, O, Ou, JJ, Chaurand, P, Caprioli, RM, Montine, TJ, Oates, JA Prostaglandin H2 (PGH2) accelerates formation of amyloid beta1-42 oligomers. J Neurochem, 82(4), 1003-6, 2002.
- Miller, M.A., Nguyen, V.Q., Lee, M-H, Kosinski, M., Schedl, T., Caprioli, R.M., Greenstein, D "A Sperm Cytoskeletal Protein that Signals Oocyte Meiotic Maturation and Ovulation." Science, 2912144-2147, 2001.
- Masumori, N, Thomas, TZ, Chaurand, P, Case, T, Paul, M, Kasper, S, Caprioli, RM, Tsukamoto, T, Shappell, SB, Matusik, RJ A probasin-large T antigen transgenic mouse line develops prostate adenocarcinoma and neuroendocrine carcinoma with metastatic potential. Cancer Res, 61(5), 2239-49, 2001.
- Miller, MA, Nguyen, VQ, Lee, MH, Kosinski, M, Schedl, T, Caprioli, RM, Greenstein, D A sperm cytoskeletal protein that signals oocyte meiotic maturation and ovulation. Science, 291(5511), 2144-7, 2001.
- Nguyen, VQ, Caprioli, RM, Cover, TL Carboxy-terminal proteolytic processing of Helicobacter pylori vacuolating toxin. Infect Immun, 69(1), 543-6, 2001.
- Nemeth, JF, Hochgesang, Jr., GP, Marnett, LJ, Caprioli, RM "Characterization of the Glycosylation Sites in Cyclooxygenase-2 Using Mass Spectrometry." Biochemistry, 103109, 2001.
- Boutaud, O., Brame, C.J., Chaurand, P., Li, J., Rowlinson, S.W., Crews, B.C., Ji, C., Marnett, L.J., Caprioli, R.M., Roberts, LJ II, Oates, J.A "Characterization of the Lysyl-Adducts of Prostaglandin H-Synthases that are Derived from Oxygenation of Arachidonic Acid." Biochemistry, 40(23), 6948-6955, 2001.
- Nemeth, JF, Hochgesang, GP, Marnett, LJ, Caprioli, RM, Hochensang, GP Characterization of the glycosylation sites in cyclooxygenase-2 using mass spectrometry. Biochemistry, 40(10), 3109-16, 2001.
- Boutaud, O, Brame, CJ, Chaurand, P, Li, J, Rowlinson, SW, Crews, BC, Ji, C, Marnett, LJ, Caprioli, RM, Roberts, LJ, Oates, JA Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid. Biochemistry, 40(23), 6948-55, 2001.
- Hondal, RJ, Ma, S, Caprioli, RM, Hill, KE, Burk, RF Heparin-binding histidine and lysine residues of rat selenoprotein P. J Biol Chem, 276(19), 15823-31, 2001.
- Stoeckli, M, Chaurand, P, Hallahan, DE, Caprioli, RM "Imaging Mass Spectrometry: A New Technique Applied to Mammalian Brain Tumor Protein Expression." Nature Medicine, 7493-496, 2001.
- Stoeckli, M, Chaurand, P, Hallahan, DE, Caprioli, RM Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nat Med, 7(4), 493-6, 2001.
- Todd, P.J., Schaaff, T.G., Chaurand, P., and Caprioli, R.M "Organic Ion Imaging of Biological Tissue with MALDI and SIMS." J. Mass Spectrom., 36(4), 355-369, 2001.
- Chaurand, P., DaGue, B.B., Pearsall, R. S., Threadgill, D.W., Caprioli, R.M "Profiling Proteins from Azoxymethane-induced Colon Tumors at the Molecular Level by MALDI Mass Spectrometry." Proteomics, 11320-1326, 2001.
- Chaurand, P, DaGue, BB, Pearsall, RS, Threadgill, DW, Caprioli, RM Profiling proteins from azoxymethane-induced colon tumors at the molecular level by matrix-assisted laser desorption/ionization mass spectrometry. Proteomics, 1(10), 1320-6, 2001.
- Chaurand, P, DaGue, BB, Ma, S, Kasper, S, Caprioli, RM Strain-based sequence variations and structure analysis of murine prostate specific spermine binding protein using mass spectrometry. Biochemistry, 40(32), 9725-33, 2001.
- Koomen, J.M., Stoeckli, M., and Caprioli, R.M "Mapping of surrogate markers of cellular components and structures using laser desorption mass spectrometry." J. Mass Spectrom, 35258-564, 2000.
- Stoeckli, M., Farmer, T.B., and Caprioli, R.M "Automated MS imaging with a MALDI TOF instrument." J. Am. Soc. Mass Spectrom, 10(1), 67-71, 1999.
- Stoeckli, M, Farmer, TB, Caprioli, RM Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument. J Am Soc Mass Spectrom, 10(1), 67-71, 1999.
- Zhang, H., Stoeckli, M., Andren, P.E., and Caprioli, R.M "Combining solid phase preconcentration, capillary electrophoresis, and imaging matrix-assisted laser desorption ionization mass spectrometry: Intracerebral metabolic processing of peptide E in vivo." J. Mass Spectrom, 342377-383, 1999.
- Zhang, H, Stoeckli, M, Andren, PE, Caprioli, RM Combining solid-phase preconcentration, capillary electrophoresis and off-line matrix-assisted laser desorption/ionization mass spectrometry: intracerebral metabolic processing of peptide E in vivo. J Mass Spectrom, 34(4), 377-83, 1999.
- Andren, P.E., and Caprioli, R.M "Determination of extracellular release of neurotensin rat brain regions utilizing in vivo microdialysis/electrospray mass spectrometry." Brian Research , 845123-129, 1999.
- Chaurand, P., Stoeckli, M. and Caprioli, R.M "Direct Profiling of Proteins in Biological Tissue Sections by MALDI Mass Spectrometry." Anal Chem, 715263-5270, 1999.
- Chaurand, P, Stoeckli, M, Caprioli, RM Direct profiling of proteins in biological tissue sections by MALDI mass spectrometry. Anal Chem, 71(23), 5263-70, 1999.
- Schlunegger, UP, Stoeckli, M, Caprioli, RM Frequency scan for the analysis of high mass ions generated by matrix-assisted laser desorption/ionization in a paul tra. Rapid Commun Mass Spectrom, 13(18), 1792-6, 1999.
- Andren, P.E., Emmett, M.R., DaGue, B.B., Steulet, A.-F., Waldmeier, P., and Caprioli, R.M "Blood-brain barrier penetration of 3-aminopropyl-n-butylphosphinic acid (CGP 36742) in rat brain by microdialysis/mass spectrometry." J. Mass Spectrom, 33281-287, 1998.
- Farmer, TB, Caprioli, RM Determination of protein-protein interactions by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom, 33(8), 697-704, 1998.
- Farmer, T.B., and Caprioli, R.M "Determination of protein-protein interactions by matrix-assisted laser desorption/ionization mass spectrometry." J. Mass Spectrom, 33(8), 697-704, 1998.
- Caprioli, R. M., Farmer, T. B., and Gile, J "Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS." Analytical Chemistry, 694751-4760, 1997.
- Zhang, H., and Caprioli, R.M "Capillary electrophoresis combined with matrix-assisted laser desorption ionization mass spectrometry: Continuous sample deposition on a matrix-precoated membrane target." J. Mass Spectrom. , 311039-1046, 1996.
- Sanderson, K., Andren, P.E., Caprioli, R.M., and Nyberg, F "In vitro metabolism of LVV-hemorphin-7 in human plasma studied by reversed-phase high-performance liquid chromatography and micro-electrospray mass spectrometry." J. Chromatogr., 743207-212, 1996.
- Eriksson, U., Andren, P.E., Caprioli, R.M., and Nyberg, F "Reversed-phase high-performance liquid chromatography combined with tandem mass spectrometry in studies of a substance P-converting enzyme from human cerebrospinal fluid." J. Chromatogr., 743213-220, 1996.
- Andren, P.E., and Caprioli, R.M "In vivo metabolism of substance P in rat striatum utilizing microdialysis/liquid chromatography/micro-electrospray mass spectrometry." J. Mass Spectrom., 30817-824, 1995.
- Lin, S.-N., Slopis, J.M., Butler, I.J., and Caprioli, R.M "In vivo microdialysis and gas chromatography/mass spectrometry for studies on release of N-acetylaspartylglutamate and N-acetylaspartate in rat brain hypothalamus." J. Neurosci. Methods, 62199-205, 1995.
- Caprioli, R.M. and Suter, M. J.-F "Mass Spectrometry." Introduction to Biophysical Methods, (Eds., J.A. Glasel and M.P. Deutscher), Academic Press Inc., Orlando, FL, 147-2041995.
- Farmer, T.B., and Caprioli, R.M "Mass discrimination in MALDI TOF MS: A study using crosslinked oligomeric complexes." J. Mass Spectrom., 301245-1254, 1995.
- Lecchi, P., and Caprioli, R.M "Matrix-assisted laser desorption mass spectrometry for peptide mapping." New Methods in Peptide Mapping for the Characterization of Proteins, (Ed. W.S. Hancock), CRC Press, Inc., Boca Raton, FL, 219-240, 1995.
- Zhang, H., Andren, P.E., and Caprioli, R.M "Micro-preparation procedure for high sensitivity matrix-assisted laser desorption ionization mass spectrometry." J. Mass Spectrom., 301768-1771, 1995.
- Emmett, M.R., Andren, P.E., and Caprioli, R.M "Specific molecular mass detection of endogenously released neuropeptides using in vivo microdialysis/mass spectrometry." J. Neuroscience , 62141-147, 1995.
- Emmett, M.R., and Caprioli, R.M "Micro-electrospray MS: Ultra-high-sensitivity analysis of peptides and proteins." J. Am. Soc. Mass Spectrom, 54605-613, 1994.
- Andren, P.E., Emmett, M.R., and Caprioli, R.M "Micro-electrospray: Zeptomole/attomole per microliter sensitivity for peptides." J. Am. Soc. Mass Spectrom,, 5867-869, 1994.
- Andren, P.E., Lin, S.-N., and Caprioli, R.M "Microdialysis/mass spectrometry." Mass Spectrometry: Clinical and Biomedical Applications, Vol. 2 (D.M. Desiderio, ed), Plenum Publish. Corp., New York, 237-254, 1994.
- Andren, P.E., and Caprioli, R.M "Microdialysis/mass spectrometry of neuropeptides." Biological Mass Spectrometry: Present and Future, (Eds. T. Matsuo, R.M. Caprioli, M. Gross, and Y. Seyama), John Wiley & Sons Ltd., Sussex, UK, 355-367, 1994.
- Caprioli, R.M "On-line LC/MS and CE/MS." Biological Mass Spectrometry: Present and Future, (Eds. T. Matsuo, R.M. Caprioli, M.L. Gross and Y. Seyama), John Wiley & Sons Ltd., Sussex, UK, 75-100, 1994.
- Farmer, T.B., and Caprioli, R.M "Assessing the multimeric states of proteins by matrix-assisted laser desorption mass spectrometry: comparison with other biochemical methods." Techniques in Protein Chemistry IV, (Ed. R.H. Angeletti), Academic Press, Inc. San Diego, CA, 73-81, 1993.
- Caprioli, R.M., and Wu, A.H.B "Mass Spectrom." Anal. Chem. Review, 65(12), 470R-474R, 1993.
- Suter, M.J.-F, Moore, W.T., Farmer, T.B., Cottrell, J.S., and Caprioli, R.M "Analysis of protein-protein binding with laser desorption mass spectrometry: Can multimeric forms be studied?." Techniques in Protein Chemistry III, (Ed. R.H. Angeletti), Academic Press, Inc., Orlando, F, 447-445, 1992.
- Suter, M.J.-F., and Caprioli, R.M "An integral probe for capillary zone electrophoresis/continuous-flow fast atom bombardment mass spectrometry." JASMS, 30198-206, 1992.
- Caprioli, R.M., and Suter, M.J.-F "Continuous-flow fast atom bombardment: Recent advances and applications." Int. J. Mass Spectrom. and Ion Processes, 118/119449-476, 1992.
- Farmer, T.B., and Caprioli, R.M "Assessing the multimeric states of proteins: Studies using laser desorption mass spectrometry." BMS, 20796-800, 1991.
- Suter, M.J.-F., DaGue, B.B., Lin, S-N, and Caprioli, R.M "Recent advances in LC/MS and CZE/MS for protein analysis." J. Chromatogr., 553101-116, 1991.
- Caprioli, R.M "Continuous-flow fast atom bombardment mass spectrometry." Biochem. CLin., 141302-1310, 1990.
- Caprioli, R.M "Continuous-flow fast atom bombardment mass spectrometry: Direct analysis of polar and ionic compounds in aqueous solutions." Anal. Chem., 62477-485, 1990.
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