Wellington Pham, Ph.D.
Assistant Professor of Radiology and Radiological Sciences
Vanderbilt University Institute of Imaging Science
1161 21st Avenue South MCN AA-1105
Nashville, TN 37232-2310
Develop molecular probes for detection of cancer using Optical, MRI and PET imaging modalities
Our ongoing investigation emphasizes on cell therapy using a transgenic and preclinical mouse model. We have employed integrated nanotechnology-based platforms to achieve a multifunctional and multiplexed nanovaccine delivery system for cancer therapy. Aside from highlighting the contrast properties and carrier features available in nanotechnology, the overall goals of our studies are to (i) provide microanatomical and functional imaging feedback of the therapeutic process and (ii) realize an approach for longitudinal treatment and monitoring.
In another project, we developed fluorescence nanoparticles for detection of colorectal cancer using fluorescence colonoscopy. Currently, the nanoparticles are prepared in GMP/GLP facilities in Japan.
Our second project focuses on the development of a novel synthetic chemistry approach to the generation of probes intended specifically for imaging Alzheimer's disease (AD). The significance of this work lies in the development of a versatile vehicle which, after being loaded with imaging cargo, can be delivered to the brain. Furthermore, our laboratory currently employes HTS and earosolized methods to facilitate the discovery of novel chemical genetics for treatment of AD. Considering the tremendous contribution made by imaging in understanding the pathogenesis of AD, the results obtained through this research will have a strong influence on current efforts to find reliable biomarkers of this disease.
- Roman V. Shchepin, Wellington Pham, Eduard Y. Chekmenev "Dephosphorylation and biodistribution of 1-13C-phospholactate in vivo." Journal of Labelled Compounds and Radiopharmaceuticals, 2014 (in press).
- Shinji Sakuma, James Yu, Timothy Quang, Ken-ichiro Hiwatari, Hironori Kumagai, Stephanie Kao, Alex Holt, Jalysa Erskind, Richard McClure, Michael Siuta, Tokio Kitamura, Seiji Koike, Kevin Wilson, Rebecca Richards-Kortum, Kay Washington, Reed Omary, John Gore and Wellington Pham "Fluorescence-based endoscopic imaging of Thomsen-Friedenreich antigen to improve early detection of colorectal cancer." Int J Cancer, 2014 (in press).
- Tokio Kitamura, Shinji Sakuma, Haruki Higashino, Yoshie Masaoka, Makoto Kataoka, Shinji Yamashita, Ken-ichiro Hiwatari, Hironori Kumagai, Naoki Morimoto, Seiji Koike, Etsuo Tobita, Robert M. Hoffman, John C. Gore and Wellington Pham "In vivo imaging of colorectal tumors on the roden instestinal mucosa by lectin-immobilized fluorescent nanospheres." Contrast Media and Molecular Imaging, 2014 ( in press).
- Richard McClure, Daijiro Yanagisawa, Donald Stec, Dave Abdollahian, Dmitry Koktysh, Dritan Xhillari, Rudolph Jaeger, Gregg Stanwood, Eduard Chekmenev, Ikuo Tooyama, John C. Gore and Wellington Pham "Inhalable Curcumin: Offering the potential for translation to imaging and treatment of Alzheimer's disease." Journal of Alzheimer's Disease, 2014 ( in press).
- Richard A McClure, Chad W Chumbley, Michelle Reyzer, Kevin Wilson, Richard M Caprioli, John C Gore and Wellington Pham "Identification of promethazine as an amyloid-binding molecule using a fluorescence high-throughput assay and MALDI imaging mass spectrometry." NeuroImage: Clinical, 2620-629, 2013 .
- Shinji Toki, Reed A. Omary, Kevin Wilson, John C. Gore, R. Stokes Peebles, Jr., Wellington Pham "A comprehensive analysis of transfection-assisted delivery of iron oxide nanoparticles to dendritic cells." Nanomedicine: Nanotechnology, Biology, and Medicine, 91235-44, 2013.
- Xie J, Wang C, Virostko J, Manning HC, Pham W, Bauer J, Gore JC "A novel reporter system for molecular imaging and high-througput screening of anticancer drugs." Chembiochem, 141494-503, 2013.
- Kumagai H, Pham W, Kataoka M, Hiwatari KI, McBride J, Wilson K, Tachikawa H, Kimura R, Nakamura K, Liu E, Gore J, Sakuma S "Multifunctional Nanobeacon for Imaging Thomsen-Friedenreich Antigen-Associated Colorectal Cancer." International Journal of Cancer, 1322107-2117, 2013.
- Friedman H, Holt AT, Pham W "Research Highlights: Highlights from the latest articles in nanomedicine." Nanomedicine, 81909-11, 2013.
- Nolting D, Nickels M, Tantawy MN, Yu JYH, Xie JP, Peterson TE, Crews BA, Marnett L, Gore JC and Pham W "Convergent synthesis and evaluation of 18F-labeled azulene COX2 probes for cancer imaging." Frontiers in Cancer Imaging and Diagnosis, 2207, 2012.
- Nolting DD, Nickels ML, Guo N, Pham W "Molecular Imaging Probe Development: A Chemistry Perspective." Am J Nucl Med Mol Imaging, 2273-311, 2012.
- Wellington Pham "Quantitative Analysis and Safety Issues of Nanotechnology in Healthcare Research." J Mol Biomark Diagn, 3(5), 1000e111, 2012.
- Sakuma S, Kataoka M, Higashino H, Yano T, Masaoka Y, Yamashita S, Hiwatari K, Kimura R, Nakamura K, Kumagai H, Gore J, and Pham W "A Potential of Peanut Agglutinin-Immobilized Fluorescent Nanospheres as a Safe Candidate of Diagnostic Drugs for Colonoscopy." European Journal of Pharmaceutical Sciences, 42(4), 340-347, 2011 .
- Sakuma S, Higashino H, Oshitani H, Masaoka Y, Kataoka M, Yamashita S, Hiwatari KI, Tachikawa H, Kimura R, Nakamura K, Kumagai H, Gore JC, Pham w "Essence of affinity and specificity of peanut agglutinin-immobilized fluorescent nanospheres with surface poly(N-vinylacetamide) chains for colorectal cancer." European Journal of Pharmaceutics and Biopharmaceutics, 79537-43, 2011 .
- Sakuma S, Yamashita S, Hiwatari KI, Hoffman RM, Pham W "Lectin-immobilized fluorescent nanospheres for targeting to colorectal cancer from a physicochemical perspective." Curr Drug Discov Technol, 8367-78, 2011 .
- Mackay, PS, Kremers GJ, Kobukai S, Cobb JG, Kuley A, Rosenthal SJ, Koktysh DS, Gore JC, Pham W "MULTIMODAL IMAGING OF DENDRITIC CELLS USING A NOVEL HYBRID MAGNETO-OPTICAL NANOPROBE." Nanomedicine:Nanotechnology, Biology, and Medicine, 7489-496, 2011 .
- Nolting DD, Gore JC and Pham W "Near-Infrared Dyes: Probe Development and Applications in Optical Molecular Imaging." Curr Org Synth, 8521-534, 2011 .
- Sampson UK, Perati RR, Prins PA, Pham W, Liu Z, Harell F, Linton MF, Gore JC, Kon V, Fazio S "Quantitative estimates of variability of in vivo ultrasound imaging measurements of mouse aorta important for studies of abdominal aortic aneurysms and related arterial diseases." J Ultras Med, 30773-784, 2011 .
- Dmitry Koktysh, Vanessa Bright, Wellington Pham "Fluorescent Magnetic Hybrid Nanoprobe for Multimodal Bioimaging." Nanotechnology, 22275606, 2011.
- Kobuka S, Kremers GJ, Cobb JG, Baheza R, Xie JP, Kuley A, Zhu M, Pham W "Induction of Antitumor Immunity by Dendritic Cells Loaded with Membrane Translocating MUCIN-1 Peptide Antigen." Translational Oncology, 4(1), 1-8, 2011.
- Nickels M, Xie JP, Cobb J, Gore JC and Pham W "Functionalization of Iron Oxide Nanoparticles With a Versatile Epoxy Amine Linker." J Mater Chem, 204776-4780, 2010.
- Kobukai S, Baheza R, Cobb J, Virostko J, Xie JP, Gillman A, Koktysh D, Kerns D, Does M, Gore JC and Pham W "Magnetic Nanoparticles for Imaging Dendritic Cells." Magn Reson Med, 631383-1390, 2010.
- Pham W, Kobukai S, Hotta C, Gore J "Dendritic Cells: Therapy and Imaging." Expert Opin Biol Ther, 9(5), 539-564, 2009.
- Nolting DD, Nickels M, Price R, Gore JC, Pham W "Synthesis of Bicyclo[5.3.0]Azulene Derivatives." Nat Protoc, 41113-1117, 2009.
- Pham W, Cassell L, Gillman A, Koktysh D and Gore JC "A Near-Infrared Dye for Multichannel Imaging." Chem Commun, 161895-1897, 2008.
- Medarova Z, Pham W, Farrar C, Petkova V, Moore A "In Vivo Imaging of siRNA Delivery and Silencing in Tumors." Nat Med, 13(3), 372-377, 2007.
- Pham W, Xie JP and Gore JC "Tracking The Migration of Dendritic Cells By In Vivo Optical Imaging." Neoplasia, 9(12), 1130-1137, 2007.
- Pham W, Pantazopoulos P, Moore A "Imaging Farnesyl Protein Transferase Using a Topologically Activated Probe." J. Am. Chem. Soc., 128(36), 11736-11737, 2006.
- Medarova Z, Pham W, Kim Y, Dai G, Moore A "In Vivo Imaging of Tumor Response to Therapy Using a Dual-Modality Imaging Strategy." Int. J. Cancer, 1182796-2802, 2006.
- Pham W, Zhao BQ, Lo EH, Medarova Z, Rosen B, Moore A. "Crossing the blood-brain barrier: a potential application of myristoylated polyarginine for in vivo neuroimaging." Neuroimage, 28287-292, 2005.
- Pham W, Medarova Z, Moore A. "Synthesis and application of a water-soluble near-infrared dye for cancer detection using optical imaging." Bioconjugate Chem., 16735-740, 2005.
- Pham W, Choi Y, Weissleder R, Tung CH "Developing a peptide-based near-infrared molecular probe for protease sensing." Bioconjugate Chem. (Special feature on imaging chemistry), 151403-1407, 2004.
- Pham W, Kircher MF, Weissleder R, Tung CH "Enhancing membrane permeability by fatty acylation of oligoarginine peptides." Chembiochem. , 51148-1151, 2004.
- Chen JW, Pham W, Weissleder R, Bogdanov A, Jr "Human myeloperoxidase: a potential target for molecular MR imaging in atherosclerosis." Magn. Reson. Med. , 521021-1028, 2004.
- Pham W, Weissleder R, Tung C-H "A practical approach for the preparation of monofunctional azulenyl squaraine dye." Tetrahedron Lett. , 443975-3978, 2003.
- Pham W, Lai WF, Weissleder R, Tung CH "High efficiency synthesis of a bioconjugatable near-infrared fluorochrome." Bioconjugate Chem. , 141048-1051, 2003.
- Pham W, Weissleder R, Tung C-H "An azulene dimer as a near-infrared quencher." Angew. Chem. Int. Ed. , 413659-3662, 2002.
- Pham W, Weissleder R, Tung C-H "Intermolecular [8+2] cycloaddition reactions of 2H-3-methoxycarbonylcyclohepta[b]furan-2-one with vinyl ethers." Tetrahedron Lett. , 4319-20, 2001.
- Krishnan V, Pham W, Messer WS, Jr., Peseckis SM "First fatty acylated dipeptides to affect muscarinic receptor ligand binding." Bioorg. Med. Chem. Lett. , 93363-3368, 1999.