| Name |
Publication |
Description |
| Human depleted serum (10/6/2008) |
IDPicker 2.0: Ma et al |
The files in directory were generated from depleted human serum processed by Lisa Zimmerman of the Ayers Institute at Vanderbilt University. Proteins were reduced and alkylated using iodoacetamide. Peptides were produced through trypsin digestion, and the three hour RPLC separations (including a two hour shallow gradient) were conducted on an LTQ Orbitrap at the Ayers Institute. Eight centroided tandem mass spectra were collected following each profile MS scan. Collection of these data was supported by U24 CA126479. |
| Human DLD1 lysate (5/28/2008) |
IDPicker 2.0: Ma et al |
The files in directory were generated from human colon adenocarcinoma cells (DLD-1 cell line) by Patrick Halvey of the Ayers Institute at Vanderbilt University. Proteins were reduced and alkylated using iodoacetamide. Peptides were produced through trypsin digestion, and the 90-minute RPLC separations (including a one hour shallow gradient) were conducted on an LTQ-XL at the Ayers Institute. Five centroided tandem mass spectra were collected following each MS scan. Collection of these data was supported by U24 CA126479. |
| SCX vs IEF of human RKO cells (6/1/2007) |
Evaluation of SCX vs IEF: Slebos et al |
These datasets were generated from the human colorectal carcinoma cell line RKO and from a primary rectal adenocarcinoma for method development (Slebos et al. J Prot. Res., Oct 2008; 7: 5286-5294). The main comparison in the paper was between strong cation exchange (SCX) and isoelectric Focusing (IEF) as a first dimension for peptide separation. These data were generated on an LTQ-Orbitrap using 10ug and 100ug quantities of RKO protein lysate. To study peptide and protein coverage by either SCX or IEF, 9 replicate runs were conducted using 50ug quantities of the primary carcinoma specimen. The study was supported by the National Cancer Institute Clinical Proteomic Technologies Assessment for Cancer program through National Institutes of Health Grant 1U24CA126479. |
