Vanderbilt-Ingram Cancer Center

The VICC.ORG Investigator Directory

Andrzej M. Krezel, Ph.D.

Assistant Professor
VICC Member
Researcher

Contact Information:

Vanderbilt University Medical Center
5270-A Medical Research Building III
Nashville, TN 37232-8240
615-322-2073

Research Specialty:

Structural NMR studies of proteins, protein-protein and protein-nucleic acid interactions

Research Description:

Our research is focused on structural studies of the specificity of protein-protein and protein-nucleic acid interactions. We use multi-dimensional solution NMR spectroscopy as a primary experimental technique. It provides details of conformation as well as molecular dynamics of proteins and nuceic acids in solution.

We focus on proteins of human pathogenic bacterium Helicobacter pylori (H. pylori). Based on our structural studies, we recently suggested biochemical functions for two previously uncharacterized proteins. H. pylori is a bacterium that infects the stomachs of around one third of the US population and half of the world population, and is probably the most common human bacterial infection. To accomplish this, H. pylori a neutralophilic organism, employes multiple mechanisms. Our long term goal is a structural and biochemical understanding of H. pylori signaling and transcriptional regulation in acidic environment.

Publications:

• Popescu, A, Karpay, A, Israel, DA, Peek, RM, Krezel, AM Helicobacter pylori protein HP0222 belongs to Arc/MetJ family of transcriptional regulators. Proteins, 59(2), 303-11, 2005.
• Whitson, SR, LeStourgeon, WM, Krezel, AM Solution structure of the symmetric coiled coil tetramer formed by the oligomerization domain of hnRNP C: implications for biological function. J Mol Biol, 350(2), 319-37, 2005.
• Chim, Nicholas, Gall, Walter E, Xiao, Jing, Harris, Mark P, Graham, Todd R, Krezel, Andrzej M Solution structure of the ubiquitin-binding domain in Swa2p from Saccharomyces cerevisiae. Proteins, 54(4), 784-93, 2004.
• Marlow, M.S., Brown, C.B., Barnett, J.V., Krezel, A.M "Solution structure of the chick TGFbeta type II receptor ligand-binding domain." Journal of Molecular Biology, 326989-997, 2003.
• Marlow, Michael S, Brown, Christopher B, Barnett, Joey V, Krezel, Andrzej M Solution structure of the chick TGFbeta type II receptor ligand-binding domain. J Mol Biol, 326(4), 989-97, 2003.
• Shahied-Milam, L., Braswell, E., LeStourgeon, W.M., Krezel, A.M "An Antiparallel Four-Helix Bundle Orients the High-affinity RNA Binding Sites in hnRNP C: A Mechanism for RNA Chaperonin Activity." Journal of Molecular Biology, 305817-828, 2001.
• Podust, L.M., Krezel, A.M., Kim, Y "Crystal Structure of C/EBPb-ATF4 bZip Heterodimer in the Absence of DNA: the Basic Region of the ATF4 bZip is an a-Helix." Journal of Biological Chemistry, 276505-513, 2001.
• Shahied, L, Braswell, E H, LeStourgeon, W M, Krezel, A M An antiparallel four-helix bundle orients the high-affinity RNA binding sites in hnRNP C: a mechanism for RNA chaperonin activity. J Mol Biol, 305(4), 817-28, 2001.
• Podust, L M, Krezel, A M, Kim, Y Crystal structure of the CCAAT box/enhancer-binding protein beta activating transcription factor-4 basic leucine zipper heterodimer in the absence of DNA. J Biol Chem, 276(1), 505-13, 2001.
• Marlow, M.S., Chim, N., Brown, C.B., Barnett, J.V., and Krezel, A.M "Letter to the editor: 1H, 13C, and 15N backbone assignments of the ligand binding domain of TGFb type II receptor." J. Biomolecular NMR , 17349-350, 2000.
• Krezel, A.M., Seymour, J.L., Lazarus, R.A., and Wagner, G "Recombinant decorsin: Dynamics of RGD recognition site." Protein Sceince, 91428-1438, 2000.
• Krezel, A M, Ulmer, J S, Wagner, G, Lazarus, R A Recombinant decorsin: dynamics of the RGD recognition site. Protein Sci, 9(8), 1428-38, 2000.
• Krezel, A.M., Kasibhatla, C., Hidalgo, P., MacKinnon, R., and Wagner, G "Solution Structure of the Potassium Channel Inhibitor Agitoxin 2: Calipers for Probing Channel Geometry." Protein Science , 41478-1489, 1995.