Vanderbilt-Ingram Cancer Center

The VICC.ORG Investigator Directory

Chin Chiang, Ph.D.

See also:

• Signal Transduction and Cell Proliferation Research Program
• Cell and Developmental Biology
• BRET: Biomedical Research Education & Training Faculty Listing
• PubMed Listing of Dr. Chiang's Publications

Professor
VICC Member
Researcher

Contact Information:

Vanderbilt University Medical Center
4110-A Medical Center North
Nashville, TN 37232-8240
615-322-9422

Research Specialty:

Our research is focused on Sonic hedgehog (Shh) signaling in development and cancer.

Research Description:

Our research is focused on Sonic hedgehog (Shh) signaling in development and cancer. We study how the distribution of Shh protein and signaling are regulated and how this regulation affects the identity and number of neural progenitors/stem cells in the central nervous system. We use the vertebrate system to address these questions, with emphasis on 1) neural progenitors involved in the establishment of the brain circuitry and 2) tumor stem cells in the initiation and progression of brain tumors such as medulloblastoma.

Publications:

• Bebesh, A.E., Fleming, J.T., Chiang, C., Carter, B.D. and Tyska, M.J. "Expression and localization of myosin-1d in the developing nervous system." Brain Res., 14409, 2012.
• Ryan K.E. and Chiang, C. "Hedgehog Secretion and Signal Transduction in Vertebrates." J. Biol. Chem., 287179015, 2012.
• Huang, X., Dubuc, A.M., Hashizume, R., Berg, J., He, Y., Chiang, C. Cooper, M.K., Northcott, P.A., Yatlor, M.D., Barnes, M.J., Tijan, T., Chen, J., Hackett, C.S., Weiss, W.A., David James, C., Rowitch, D.H., Shuman, M.A., Jan, Y.H. and Jan L.Y. "Voltage-gated potassium channel EAG2 controls mitotic entry and tumor growth in medulloblastoma via regulating cell volume dynamics.." Genes Dev., 261780, 2012.
• Cheng, F.Y., Huang, X., Sarangi, A., Ketova, T., Cooper, M.K., Litingtung, Y. and Chiang, C. "Widespread contribution of Gdf7 lineage to cerebellar cell types and implications for Hedgehog-driven medulloblastoma formation.." Plos One, 735541, 2012.
• Mason, T.A., Kolobova, E., Liu, J., Roland, J.T., Chiang, C. "Darinaparsin is a multivalent chemotherapeutic which induces incomplete stress response with disruption of microtubules and Shh signaling." Plos One, 6(11), 27699, 2011.
• Huang, X., Sarangi, A., Ketova, T., Cooper, M. K., Litingtung, Y. and Chiang, C. "Intracranial Orthotopic Allografting of Medulloblastoma.." J. Vis. Exp., 442153, 2010.
• Huang, X., Ketova, T., Litingtung, Y. and Chiang, C "Isolation, Enrichment and Maintenance of Medulloblastoma Stem Cells.." J. Vis. Exp., 432086, 2010.
• Huang, X, Liu, J, Ketova, T, Fleming, JT, Grover, VK, Cooper, MK, Litingtung, Y, Chiang, C Transventricular delivery of Sonic hedgehog is essential to cerebellar ventricular zone development. Proc Natl Acad Sci U S A, 107(18), 8422-7, 2010.
• Oh, S, Huang, X, Liu, J, Litingtung, Y, Chiang, C Shh and Gli3 activities are required for timely generation of motor neuron progenitors. Dev Biol, 331(2), 261-9, 2009.
• Huang, X, Ketova, T, Fleming, JT, Wang, H, Dey, SK, Litingtung, Y, Chiang, C Sonic hedgehog signaling regulates a novel epithelial progenitor domain of the hindbrain choroid plexus. Development, 2009.
• Li, Y, Gordon, J, Manley, NR, Litingtung, Y, Chiang, C Bmp4 is required for tracheal formation: A novel mouse model for tracheal agenesis. Dev Biol, 2008.
• Huang, X, Goudy, SL, Ketova, T, Litingtung, Y, Chiang, C Gli3-deficient mice exhibit cleft palate associated with abnormal tongue development. Dev Dyn, 237(10), 3079-87, 2008.
• Fogel, JL, Chiang, C, Huang, X, Agarwala, S Ventral specification and perturbed boundary formation in the mouse midbrain in the absence of Hedgehog signaling. Dev Dyn, 237(5), 1359-72, 2008.
• Guerrero, I and Chiang, C. A conserved mechanism of Hedgehog gradient formation by lipid modifications. Trends in Cell Biology, 171-5, 2007.
• Li, Y, Litingtung, Y, Ten Dijke, P, Chiang, C Aberrant Bmp signaling and notochord delamination in the pathogenesis of esophageal atresia. Dev Dyn, 236(3), 746-54, 2007.
• Huang, X, Litingtung, Y, Chiang, C Ectopic sonic hedgehog signaling impairs telencephalic dorsal midline development: implication for human holoprosencephaly. Hum Mol Genet, 16(12), 1454-68, 2007.
• Zhang, H, Lawson, WE, Polosukhin, VV, Pozzi, A, Blackwell, TS, Litingtung, Y, Chiang, C Inhibitor of differentiation 1 promotes endothelial survival in a bleomycin model of lung injury in mice. Am J Pathol, 171(4), 1113-26, 2007.
• Huang, X, Litingtung, Y, Chiang, C Region-specific requirement for cholesterol modification of sonic hedgehog in patterning the telencephalon and spinal cord. Development, 134(11), 2095-105, 2007.
• Li, Y, Zhang, H, Litingtung, Y, Chiang, C Cholesterol modification restricts the spread of Shh gradient in the limb bud. Proc Natl Acad Sci U S A, 103(17), 6548-53, 2006.
• Chiang, C "From Oligodactyly to Polydactyly:role of Shh and Gli3 in limb morphogenesis. In Hedgehog-Gli Signaling and Human Disease, Edited by A. Ruiz I Altaba." , 137-145, 2006.
• Zhang, H., Litingtung, Y. and Chiang, C. "Sonic Hedgehog signaling in viceral organ development. In Shh and Gli signaling in Development. Edited by S. Howie and C. Fisher." , 2006.
• van Bokhoven, H, Celli, J, van Reeuwijk, J, Rinne, T, Glaudemans, B, van Beusekom, E, Rieu, P, Newbury-Ecob, RA, Chiang, C, Brunner, HG MYCN haploinsufficiency is associated with reduced brain size and intestinal atresias in Feingold syndrome. Nat Genet, 37(5), 465-7, 2005.
• Oh, Saeock, Huang, Xi, Chiang, Chin Specific requirements of sonic hedgehog signaling during oligodendrocyte development. Dev Dyn, 234(3), 489-96, 2005.
• Powles, N, Marshall, H, Economou, A, Chiang, C, Murakami, A, Dickson, C, Krumlauf, R, Maconochie, M Regulatory analysis of the mouse Fgf3 gene: control of embryonic expression patterns and dependence upon sonic hedgehog (Shh) signalling. Dev Dyn, 230(1), 44-56, 2004.
• Li, Yina, Zhang, Huimin, Choi, Seung Cheol, Litingtung, Ying, Chiang, Chin Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis. Dev Biol, 270(1), 214-31, 2004.
• Chiang, CW, Kanies, C, Kim, KW, Fang, WB, Parkhurst, C, Xie, M, Henry, T, Yang, E Protein phosphatase 2A dephosphorylation of phosphoserine 112 plays the gatekeeper role for BAD-mediated apoptosis. Mol Cell Biol, 23(18), 6350-62, 2003.
• Ohkubo, Y, Chiang, C, Rubenstein, JL Coordinate regulation and synergistic actions of BMP4, SHH and FGF8 in the rostral prosencephalon regulate morphogenesis of the telencephalic and optic vesicles. Neuroscience, 111(1), 1-17, 2002.
• Litingtung, Ying, Dahn, Randall D, Li, Yina, Fallon, John F, Chiang, Chin Shh and Gli3 are dispensable for limb skeleton formation but regulate digit number and identity. Nature, 418(6901), 979-83, 2002.
• Liu, W, Li, G, Chien, JS, Raft, S, Zhang, H, Chiang, C, Frenz, DA Sonic hedgehog regulates otic capsule chondrogenesis and inner ear development in the mouse embryo. Dev Biol, 248(2), 240-50, 2002.
• Perriton, CL, Powles, N, Chiang, C, Maconochie, MK, Cohn, MJ Sonic hedgehog signaling from the urethral epithelium controls external genital development. Dev Biol, 247(1), 26-46, 2002.
• Mo, R, Kim, JH, Zhang, J, Chiang, C, Hui, CC, Kim, PC Anorectal malformations caused by defects in sonic hedgehog signaling. Am J Pathol, 159(2), 765-74, 2001.
• Chattopadhyay, A, Chiang, CW, Yang, E BAD/BCL-[X(L)] heterodimerization leads to bypass of G0/G1 arrest. Oncogene, 20(33), 4507-18, 2001.
• Chiang, C, Litingtung, Y, Harris, M P, Simandl, B K, Li, Y, Beachy, P A, Fallon, J F Manifestation of the limb prepattern: limb development in the absence of sonic hedgehog function. Dev Biol, 236(2), 421-35, 2001.
• Chiang, CW, Harris, G, Ellig, C, Masters, SC, Subramanian, R, Shenolikar, S, Wadzinski, BE, Yang, E Protein phosphatase 2A activates the proapoptotic function of BAD in interleukin- 3-dependent lymphoid cells by a mechanism requiring 14-3-3 dissociation. Blood, 97(5), 1289-97, 2001.
• Litingtung, Y, Chiang, C Control of Shh activity and signaling in the neural tube. Dev Dyn, 219(2), 143-54, 2000.
• Pak, JH, Huang, FL, Li, J, Balschun, D, Reymann, KG, Chiang, C, Westphal, H, Huang, KP Involvement of neurogranin in the modulation of calcium/calmodulin-dependent protein kinase II, synaptic plasticity, and spatial learning: a study with knockout mice. Proc Natl Acad Sci U S A, 97(21), 11232-7, 2000.
• Litingtung, Y, Chiang, C Specification of ventral neuron types is mediated by an antagonistic interaction between Shh and Gli3. Nat Neurosci, 3(10), 979-85, 2000.
• Pierani, A, Brenner-Morton, S, Chiang, C, Jessell, TM A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord. Cell, 97(7), 903-15, 1999.
• Chiang, C, Swan, RZ, Grachtchouk, M, Bolinger, M, Litingtung, Y, Robertson, EK, Cooper, MK, Gaffield, W, Westphal, H, Beachy, PA, Dlugosz, AA Essential role for Sonic hedgehog during hair follicle morphogenesis. Dev Biol, 205(1), 1-9, 1999.
• Borycki, AG, Brunk, B, Tajbakhsh, S, Buckingham, M, Chiang, C, Emerson, CP Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development, 126(18), 4053-63, 1999.
• Kos, L, Chiang, C, Mahon, KA Mediolateral patterning of somites: multiple axial signals, including Sonic hedgehog, regulate Nkx-3.1 expression. Mech Dev, 70(1-2), 25-34, 1998.
• Litingtung, Y, Lei, L, Westphal, H, Chiang, C Sonic hedgehog is essential to foregut development. Nat Genet, 20(1), 58-61, 1998.
• Knezevic, V., De Santo, R., Schughart, K., Huffstadt, U., Chiang, C., Mahon, K.A. and Mackem, S "Hoxd-12 differentially affects preaxial and postaxial chondrogenic branches in the limb and regulates sonic hedgehog in a positive feedback loop." Development, 1244523-4536, 1997.
• Chiang C, Litingtung Y, Lee E, Young K, Corden JL, Westphal H and Beachy PA "Cyclopia and defective axial patterning in mice lacking Sonic Hedgehog gene function." Nature, 383407-413, 1996.
• Yuryev, A, Patturajan, M, Litingtung, Y, Joshi, RV, Gentile, C, Gebara, M, Corden, JL The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins. Proc Natl Acad Sci U S A, 93(14), 6975-80, 1996.
• Roelink, H., Porter, J., Chiang, C., Tanabe, Y., Chang, D.T., Beachy, P.A., and Jessell, T.M. "Concentration-dependent induction of floor plate and motor neuron differentiation by the amino terminal autoproteolytic fragment of Sonic Hedgehog.." Cell, 81445-455, 1995.
• Chiang, C., Yong, K.E., and Beachy, P.A. "Controlof drosphila trachael branching by the novel homeodomain gene unplugges, a regulatory target for genes of the bithorax complex.." Development, 1213901-3912, 1995.
• Hynes, M., Porter, J.A., Chiang, C, Chang, D., Tessier-Lavigne, M., Beachy P.A., and Rosenthal, A. "Induction of midbrain dopaminergic neurons product of hedgehog autoproteolysis." Neuron, 1535-44, 1995.
• Lopez, A., Chang, D.T., Chiang, C., Porter, J., maria, A.R., Simandl, K., Beachy, P.A., and Fallon, J.F. "Limb patterning activity and restricted posterior localization of the amino terminal product of hedgehog autoproteolysis." Current Biology, 5791-796, 1995.
• Fan, C.-M., Porter, J.A., Chiang, C., Chang, D.T., Beachy, P.A., Tessier,-Lavigne, M. "Long range sclerotome induction by Sonic Hedgehog: direct role of the amino-terminal clleavage product and modulation by the cyclic AMP signalling pathway." Cell, 81457-465, 1995.
• Chiang, C., andBeachy, P.A "Expression of a novel Toll-like gene spans the parasegment boundary and contributes to hedgehog function in the adult eye of Drosophila." Mech. Dev., 47225-239, 1994.
• Chang, D.T., Lopez, A., von Kessler, D.P., Chiang, C., Simandl, K., Renbin, Z., Seldin, M.F., Fallon, J.F., and Beachy, P.A "Products, genetic linkage, and limb patterning activity of a murine hedgehog gene.." Development, 1203339-3353, 1994.
• Chiang, C.,, Patel, N., Young, K., and Beachy, P.A. "The novel homeodomain gene buttonless specifies differentiation and axonal guidance functions of Drosophila dorsal median cells." Development, 1203581-3593, 1994.