Vanderbilt-Ingram Cancer Center

The VICC.ORG Investigator Directory

James E. Crowe, Jr., M.D.

See also:

• Host Tumor Interactions Research Program
• Pediatric Infectious Disease
• BRET: Biomedical Research Education & Training Faculty Listing
• PubMed Listing of Dr. Crowe's Publications

Ingram Professor of Cancer Research
Professor of Pediatrics, Microbiology and Immuno.
Director, Vanderbilt Alliance for Nanomedicine
Director, Vanderbilt Program in Vaccine Sciences
VICC Member
Researcher

Contact Information:

Vanderbilt University Medical Center
11475 Medical Research Building IV - 2213 Garland Ave.
Nashville, TN 37232-2905
615-343-8064

Research Description:

Our laboratory studies focus on the molecular basis for the development of effective B cell responses to viruses in humans, and on the cell biology of virus infection in polarized epithelial cells. We also have active programs in biodefense research, vaccine and monoclonal antibody development, nanomedicine, and biotechnology. Our group uses a very broad array of techniques including molecular and cellular biology, state-of-the-art imaging and flow cytometry, bioinformatics, and bioengineering approaches to attack the scientific problems that are of interest to us. Our philosophy is to work on major human pathogens - we study model systems only when the direct study of the primary pathogen in humans is not feasible at a definitive level. Current studies center on influenza, dengue virus, HIV, respiratory syncytial virus, human metapneumovirus, rotavirus, Rift Valley Fever virus, West Nile virus, and vaccinia virus/smallpox.

A first area of emphasis is the study of the genetic origins and structural determinants of virus neutralizing antibodies. To study this area we have developed unique B cell and antibody engineering techniques including phage display combinatorial libraries, novel methods for generation of human B cell hybridomas, direct cloning of antibody variable genes from virus-specific B cells, human hybridoma methods, high throughput sequence analysis, and full-length expression of recombinant antibodies. This work has allowed us to understand better structure/function characteristics of antiviral antibodies. Several of the recombinant human antibodies that we have generated are being developed commercially.

We have a second major program in the study of the trafficking of virus proteins in polarized epithelial cells. We have recently discovered that a unique cell compartment, the apical recycling endosome, controls directional budding of respiratory syncytial virus. We are studying the role of particular proteins, including Rabs, Rab-FIPs, and myosins on viral trafficking. We also have investigated the role of stress granules in RSV infection. Currently, scientists are exploring the role of viral proteins in controlling these processes. This work requires careful biochemistry, confocal and electron microscopy, and molecular biology.

Clinical Interest:

Immunology of infectious diseases. Infant respiratory virus infections due to RSV and metapneumovirus.

Publications:

• Shaikh, FY, Cox, RG, Lifland, AW, Hotard, AL, Williams, JV, Moore, ML, Santangelo, PJ, Crowe, JE A critical phenylalanine residue in the respiratory syncytial virus fusion protein cytoplasmic tail mediates assembly of internal viral proteins into viral filaments and particles. MBio, 3(1), 2012.
• Briney, BS, Willis, JR, Hicar, MD, Thomas, JW, Crowe, JE Frequency and genetic characterization of V(DD)J recombinants in the human peripheral blood antibody repertoire. Immunology, 137(1), 56-64, 2012.
• Briney, BS, Willis, JR, McKinney, BA, Crowe, JE High-throughput antibody sequencing reveals genetic evidence of global regulation of the naïve and memory repertoires that extends across individuals. Genes Immun, 2012.
• Krause, JC, Tsibane, T, Tumpey, TM, Huffman, CJ, Albrecht, R, Blum, DL, Ramos, I, Fernandez-Sesma, A, Edwards, KM, García-Sastre, A, Basler, CF, Crowe, JE Human monoclonal antibodies to pandemic 1957 H2N2 and pandemic 1968 H3N2 influenza viruses. J Virol, 86(11), 6334-40, 2012.
• Briney, BS, Willis, JR, Crowe, JE Human peripheral blood antibodies with long HCDR3s are established primarily at original recombination using a limited subset of germline genes. PLoS One, 7(5), e36750, 2012.
• Crowe, JE Influenza virus resistance to human neutralizing antibodies. MBio, 3(4), 2012.
• Briney, BS, Willis, JR, Crowe, JE Location and length distribution of somatic hypermutation-associated DNA insertions and deletions reveals regions of antibody structural plasticity. Genes Immun, 2012.
• Smith, SA, Zhou, Y, Olivarez, NP, Broadwater, AH, de Silva, AM, Crowe, JE Persistence of circulating memory B cell clones with potential for dengue virus disease enhancement for decades following infection. J Virol, 86(5), 2665-75, 2012.
• Engelhardt, BG, Jagasia, SM, Crowe, JE, Griffith, ML, Savani, BN, Kassim, AA, Lu, P, Weitkamp, JH, Moore, DJ, Yoder, SM, Rock, MT, Jagasia, M Predicting posttransplantation diabetes mellitus by regulatory T-cell phenotype: implications for metabolic intervention to modulate alloreactivity. Blood, 119(10), 2417-21, 2012.
• Shaikh, FY, Utley, TJ, Craven, RE, Rogers, MC, Lapierre, LA, Goldenring, JR, Crowe, JE Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. PLoS One, 7(7), e40826, 2012.
• Engelhardt, BG, Sengsayadeth, SM, Jagasia, M, Savani, BN, Kassim, AA, Lu, P, Shyr, Y, Yoder, SM, Rock, MT, Crowe, JE Tissue-specific regulatory T cells; biomarker for acute graft-versus-host disease and survival. Exp Hematol, 2012.
• Krause, JC, Tsibane, T, Tumpey, TM, Huffman, CJ, Basler, CF, Crowe, JE A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of the influenza H1N1 virus hemagglutinin. J Virol, 85(20), 10905-8, 2011.
• Lindquist, ME, Mainou, BA, Dermody, TS, Crowe, JE Activation of protein kinase R is required for induction of stress granules by respiratory syncytial virus but dispensable for viral replication. Virology, 413(1), 103-10, 2011.
• Krause, JC, Ekiert, DC, Tumpey, TM, Smith, PB, Wilson, IA, Crowe, JE An insertion mutation that distorts antibody binding site architecture enhances function of a human antibody. MBio, 2(1), e00345-10, 2011.
• Krause, JC, Tsibane, T, Tumpey, TM, Huffman, CJ, Briney, BS, Smith, SA, Basler, CF, Crowe, JE Epitope-specific human influenza antibody repertoires diversify by B cell intraclonal sequence divergence and interclonal convergence. J Immunol, 187(7), 3704-11, 2011.
• Rock, MT, McKinney, BA, Yoder, SM, Prudom, CE, Wright, DW, Crowe, JE Identification of potential human respiratory syncytial virus and metapneumovirus T cell epitopes using computational prediction and MHC binding assays. J Immunol Methods, 374(1-2), 13-7, 2011.
• Joyner, AS, Willis, JR, Crowe, JE, Aiken, C Maturation-induced cloaking of neutralization epitopes on HIV-1 particles. PLoS Pathog, 7(9), e1002234, 2011.
• Hicar, MD, Kalams, SA, Spearman, PW, Crowe, JE Emerging studies of human HIV-specific antibody repertoires. Vaccine, 28 Suppl 2B18-23, 2010.
• Engelhardt, BG, Crowe, JE Homing in on Acute Graft vs. Host Disease: Tissue-Specific T Regulatory and Th17 Cells. Curr Top Microbiol Immunol, 2010.
• Krause, JC, Tumpey, TM, Huffman, CJ, McGraw, PA, Pearce, MB, Tsibane, T, Hai, R, Basler, CF, Crowe, JE Naturally occurring human monoclonal antibodies neutralize both 1918 and 2009 pandemic influenza A (H1N1) viruses. J Virol, 84(6), 3127-30, 2010.
• Williams, JV, Edwards, KM, Weinberg, GA, Griffin, MR, Hall, CB, Zhu, Y, Szilagyi, PG, Wang, CK, Yang, CF, Silva, D, Ye, D, Spaete, RR, Crowe, JE Population-based incidence of human metapneumovirus infection among hospitalized children. J Infect Dis, 201(12), 1890-8, 2010.
• Hicar, MD, Chen, X, Briney, B, Hammonds, J, Wang, JJ, Kalams, S, Spearman, PW, Crowe, JE Pseudovirion particles bearing native HIV envelope trimers facilitate a novel method for generating human neutralizing monoclonal antibodies against HIV. J Acquir Immune Defic Syndr, 54(3), 223-35, 2010.
• Engelhardt, BG, Jagasia, M, Savani, BN, Bratcher, NL, Greer, JP, Jiang, A, Kassim, AA, Lu, P, Schuening, F, Yoder, SM, Rock, MT, Crowe, JE Regulatory T cell expression of CLA or alpha(4)beta(7) and skin or gut acute GVHD outcomes. Bone Marrow Transplant, 2010.
• Lindquist, ME, Lifland, AW, Utley, TJ, Santangelo, PJ, Crowe, JE Respiratory syncytial virus induces host RNA stress granules to facilitate viral replication. J Virol, 84(23), 12274-84, 2010.
• Pishchany, G, McCoy, AL, Torres, VJ, Krause, JC, Crowe, JE, Fabry, ME, Skaar, EP Specificity for human hemoglobin enhances Staphylococcus aureus infection. Cell Host Microbe, 8(6), 544-50, 2010.
• Thornburg, NJ, Shepherd, B, Crowe, JE Transforming growth factor beta is a major regulator of human neonatal immune responses following respiratory syncytial virus infection. J Virol, 84(24), 12895-902, 2010.
• Moore, ML, Chi, MH, Luongo, C, Lukacs, NW, Polosukhin, VV, Huckabee, MM, Newcomb, DC, Buchholz, UJ, Crowe, JE, Goleniewska, K, Williams, JV, Collins, PL, Peebles, RS A Chimeric A2 Strain Respiratory Syncytial Virus (RSV) with the Fusion Protein of RSV Strain Line 19 Exhibits Enhanced Viral Load, Mucus, and Airway Dysfunction. J Virol, 2009.
• Talbot, HK, Crowe, JE, Edwards, KM, Griffin, MR, Zhu, Y, Weinberg, GA, Szilagyi, PG, Hall, CB, Podsiad, AB, Iwane, M, Williams, JV, , Coronavirus infection and hospitalizations for acute respiratory illness in young children. J Med Virol, 81(5), 853-6, 2009.
• Yang, CF, Wang, CK, Tollefson, SJ, Piyaratna, R, Lintao, LD, Chu, M, Liem, A, Mark, M, Spaete, RR, Crowe, JE, Williams, JV Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years. Virol J, 6138, 2009.
• Crowe, JE Recent advances in the study of human antibody responses to influenza virus using optimized human hybridoma approaches. Vaccine, 27 Suppl 6G47-51, 2009.
• Talbot, HK, Shepherd, BE, Crowe, JE, Griffin, MR, Edwards, KM, Podsiad, AB, Tollefson, SJ, Wright, PF, Williams, JV The pediatric burden of human coronaviruses evaluated for twenty years. Pediatr Infect Dis J, 28(8), 682-7, 2009.
• Mok, H, Tollefson, SJ, Podsiad, AB, Shepherd, BE, Polosukhin, VV, Johnston, RE, Williams, JV, Crowe, JE An alphavirus replicon-based human metapneumovirus vaccine is immunogenic and protective in mice and cotton rats. J Virol, 82(22), 11410-8, 2008.
• Yu, X, McGraw, PA, House, FS, Crowe, JE An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies. J Immunol Methods, 336(2), 142-51, 2008.
• Mok, H, Lee, S, Wright, DW, Crowe, JE Enhancement of the CD8(+) T cell response to a subdominant epitope of respiratory syncytial virus by deletion of an immunodominant epitope. Vaccine, 2008.
• Kallewaard, NL, McKinney, BA, Gu, Y, Chen, A, Prasad, BV, Crowe, JE Functional maturation of the human antibody response to rotavirus. J Immunol, 180(6), 3980-9, 2008.
• Derdowski, A, Peters, TR, Glover, N, Qian, R, Utley, TJ, Burnett, A, Williams, JV, Spearman, P, Crowe, JE Human metapneumovirus nucleoprotein and phosphoprotein interact and provide the minimal requirements for inclusion body formation. J Gen Virol, 89(Pt 11), 2698-708, 2008.
• Tian, C, Luskin, GK, Dischert, KM, Higginbotham, JN, Shepherd, BE, Crowe, JE Immunodominance of the VH1-46 antibody gene segment in the primary repertoire of human rotavirus-specific B cells is reduced in the memory compartment through somatic mutation of nondominant clones. J Immunol, 180(5), 3279-88, 2008.
• Yu, X, Tsibane, T, McGraw, PA, House, FS, Keefer, CJ, Hicar, MD, Tumpey, TM, Pappas, C, Perrone, LA, Martinez, O, Stevens, J, Wilson, IA, Aguilar, PV, Altschuler, EL, Basler, CF, Crowe, JE Neutralizing antibodies derived from the B cells of 1918 influenza pandemic survivors. Nature, 455(7212), 532-6, 2008.
• Utley, TJ, Ducharme, NA, Varthakavi, V, Shepherd, BE, Santangelo, PJ, Lindquist, ME, Goldenring, JR, Crowe, JE Respiratory syncytial virus uses a Vps4-independent budding mechanism controlled by Rab11-FIP2. Proc Natl Acad Sci U S A, 105(29), 10209-14, 2008.
• Williams, JV, Chen, Z, Cseke, G, Wright, DW, Keefer, CJ, Tollefson, SJ, Hessell, A, Podsiad, A, Shepherd, BE, Sanna, PP, Burton, DR, Crowe, JE, Williamson, RA A recombinant human monoclonal antibody to human metapneumovirus fusion protein that neutralizes virus in vitro and is effective therapeutically in vivo. J Virol, 81(15), 8315-24, 2007.
• Tian, C, Luskin, GK, Dischert, KM, Higginbotham, JN, Shepherd, BE, Crowe, JE Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naïve and memory B cells. Mol Immunol, 44(9), 2173-83, 2007.
• Miller, SA, Tollefson, S, Crowe, JE, Williams, JV, Wright, DW Examination of a fusogenic hexameric core from human metapneumovirus and identification of a potent synthetic peptide inhibitor from the heptad repeat 1 region. J Virol, 81(1), 141-9, 2007.
• Cseke, G, Wright, DW, Tollefson, SJ, Johnson, JE, Crowe, JE, Williams, JV Human metapneumovirus fusion protein vaccines that are immunogenic and protective in cotton rats. J Virol, 81(2), 698-707, 2007.
• Mok, H, Lee, S, Utley, TJ, Shepherd, BE, Polosukhin, VV, Collier, ML, Davis, NL, Johnston, RE, Crowe, JE Venezuelan Equine Encephalitis Replicon Particles Encoding Respiratory Syncytial Virus Surface Glycoproteins Induce Protective Mucosal Responses in Mice and Cotton Rats. J Virol, 2007.
• Rock, MT, Yoder, SM, Talbot, TR, Edwards, KM, Crowe, JE Cellular immune responses to diluted and undiluted aventis pasteur smallpox vaccine. J Infect Dis, 194(4), 435-43, 2006.
• McKinney, BA, Reif, DM, Rock, MT, Edwards, KM, Kingsmore, SF, Moore, JH, Crowe, JE Cytokine expression patterns associated with systemic adverse events following smallpox immunization. J Infect Dis, 194(4), 444-53, 2006.
• McKinney, BA, Crowe, JE, Voss, HU, Crooke, PS, Barney, N, Moore, JH Hybrid grammar-based approach to nonlinear dynamical system identification from biological time series. Phys Rev E Stat Nonlin Soft Matter Phys, 73(2 Pt 1), 021912, 2006.
• Tian, C, Kron, GK, Dischert, KM, Higginbotham, JN, Crowe, JE Low expression of the interleukin (IL)-4 receptor alpha chain and reduced signalling via the IL-4 receptor complex in human neonatal B cells. Immunology, 119(1), 54-62, 2006.
• Williams, JV, Wang, CK, Yang, CF, Tollefson, SJ, House, FS, Heck, JM, Chu, M, Brown, JB, Lintao, LD, Quinto, JD, Chu, D, Spaete, RR, Edwards, KM, Wright, PF, Crowe, JE The role of human metapneumovirus in upper respiratory tract infections in children: a 20-year experience. J Infect Dis, 193(3), 387-95, 2006.
• Lee, S, Miller, SA, Wright, DW, Rock, MT, Crowe, JE Tissue-Specific Regulation of CD8+ T Lymphocyte Immunodominance in Respiratory Syncytial Virus Infection. J Virol, 2006.
• Williams, JV, Martino, R, Rabella, N, Otegui, M, Parody, R, Heck, JM, Crowe, JE A prospective study comparing human metapneumovirus with other respiratory viruses in adults with hematologic malignancies and respiratory tract infections. J Infect Dis, 192(6), 1061-5, 2005.
• Kallewaard, NL, Bowen, AL, Crowe, JE Cooperativity of actin and microtubule elements during replication of respiratory syncytial virus. Virology, 331(1), 73-81, 2005.
• Rock, MT, Yoder, SM, Wright, PF, Talbot, TR, Edwards, KM, Crowe, JE Differential regulation of granzyme and perforin in effector and memory T cells following smallpox immunization. J Immunol, 174(6), 3757-64, 2005.
• Williams, JV, Crowe, JE, Enriquez, R, Minton, P, Peebles, RS, Hamilton, RG, Higgins, S, Griffin, M, Hartert, TV Human metapneumovirus infection plays an etiologic role in acute asthma exacerbations requiring hospitalization in adults. J Infect Dis, 192(7), 1149-53, 2005.
• Weitkamp, JH, Lafleur, BJ, Greenberg, HB, Crowe, JE Natural evolution of a human virus-specific antibody gene repertoire by somatic hypermutation requires both hotspot-directed and randomly-directed processes. Hum Immunol, 66(6), 666-76, 2005.
• Tai, JH, Williams, JV, Edwards, KM, Wright, PF, Crowe, JE, Dermody, TS Prevalence of reovirus-specific antibodies in young children in nashville, tennessee. J Infect Dis, 191(8), 1221-4, 2005.
• Bentzen, EL, House, F, Utley, TJ, Crowe, JE, Wright, DW Progression of respiratory syncytial virus infection monitored by fluorescent quantum dot probes. Nano Lett, 5(4), 591-5, 2005.
• Chen, X, Rock, MT, Hammonds, J, Tartaglia, J, Shintani, A, Currier, J, Slike, B, Crowe, JE, Marovich, M, Spearman, P Pseudovirion particle production by live poxvirus human immunodeficiency virus vaccine vector enhances humoral and cellular immune responses. J Virol, 79(9), 5537-47, 2005.
• Weitkamp, JH, Lafleur, BJ, Crowe, JE Rotavirus-specific CD5+ B cells in young children exhibit a distinct antibody repertoire compared with CD5- B cells. Hum Immunol, 67(1-2), 33-42, 2005.
• Shaklee, JF, Talbot, TR, Muldowney, JA, Vaughan, DE, Butler, J, House, F, Crowe, JE, Smith, LH, Edwards, KM Smallpox vaccination does not elevate systemic levels of prothrombotic proteins associated with ischemic cardiac events. J Infect Dis, 191(5), 724-30, 2005.
• Williams, JV, Tollefson, SJ, Johnson, JE, Crowe, JE The cotton rat (Sigmodon hispidus) is a permissive small animal model of human metapneumovirus infection, pathogenesis, and protective immunity. J Virol, 79(17), 10944-51, 2005.
• Brock, SC, Heck, JM, McGraw, PA, Crowe, JE The transmembrane domain of the respiratory syncytial virus F protein is an orientation-independent apical plasma membrane sorting sequence. J Virol, 79(19), 12528-35, 2005.
• Bowen, AL, Tian, C, LaFleur, BJ, Crowe, JE Transcriptional control of activation-induced cytidine deaminase and error-prone DNA polymerases is functionally mature in the B cells of infants at birth. Hum Immunol, 67(1-2), 43-6, 2005.
• Weitkamp, JH, Kallewaard, NL, Bowen, AL, Lafleur, BJ, Greenberg, HB, Crowe, JE VH1-46 is the dominant immunoglobulin heavy chain gene segment in rotavirus-specific memory B cells expressing the intestinal homing receptor alpha4beta7. J Immunol, 174(6), 3454-60, 2005.
• Rock, Michael T, Yoder, Sandra M, Talbot, Thomas R, Edwards, Kathryn M, Crowe, James E Adverse events after smallpox immunizations are associated with alterations in systemic cytokine levels. J Infect Dis, 189(8), 1401-10, 2004.
• Suara, Rahaman O, Crowe, James E Effect of zinc salts on respiratory syncytial virus replication. Antimicrob Agents Chemother, 48(3), 783-90, 2004.
• Williams, John V, Harris, Paul A, Tollefson, Sharon J, Halburnt-Rush, Lisa L, Pingsterhaus, Joyce M, Edwards, Kathryn M, Wright, Peter F, Crowe, James E Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children. N Engl J Med, 350(5), 443-50, 2004.
• Crowe, JE Human metapneumovirus as a major cause of human respiratory tract disease. Pediatr Infect Dis J, 23(11 Suppl), S215-21, 2004.
• Talbot, TR, Stapleton, JT, Brady, RC, Winokur, PL, Bernstein, DI, Germanson, T, Yoder, SM, Rock, MT, Crowe, JE, Edwards, KM Vaccination success rate and reaction profile with diluted and undiluted smallpox vaccine: a randomized controlled trial. JAMA, 292(10), 1205-12, 2004.
• Brock, Sean C, Goldenring, James R, Crowe, James E Apical recycling systems regulate directional budding of respiratory syncytial virus from polarized epithelial cells. Proc Natl Acad Sci U S A, 100(25), 15143-8, 2003.
• Crowe, James E., Sannella, Edith C., Pfeiffer, Steffen, Zorn, George L., Azimzadeh, Agnes, Newman, Roland, Miller, Geraldine G., Pierson, Richard N CD154 Regulates Primate Humoral Immunity to Influenza. Am J Transplant, 3680-688, 2003.
• Halasa, Natasha B, Whitlock, James A, McCurley, Thomas L, Smith, Jarrod A, Zhu, Quili, Ochs, Hans, Dermody, Terence S, Crowe, James E Fatal hemophagocytic lymphohistiocytosis associated with Epstein-Barr virus infection in a patient with a novel mutation in the signaling lymphocytic activation molecule-associated protein. Clin Infect Dis, 37(10), e136-41, 2003.
• Weitkamp, Jörn Hendrik, Kallewaard, Nicole, Kusuhara, Koichi, Feigelstock, Dino, Feng, Ninguo, Greenberg, Harry B, Crowe, James E Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. J Immunol Methods, 275223-37, 2003.
• Rock, Michael T, Crowe, James E Identification of a novel human leucocyte antigen-A*01-restricted cytotoxic T-lymphocyte epitope in the respiratory syncytial virus fusion protein. Immunology, 108474-80, 2003.
• Crowe, James E, Williams, John V Immunology of viral respiratory tract infection in infancy. Paediatr Respir Rev, 4112-9, 2003.
• Weitkamp, Joern-Hendrik, Kallewaard, Nicole, Kusuhara, Koichi, Bures, Elizabeth, Williams, John V, LaFleur, Bonnie, Greenberg, Harry B, Crowe, James E Infant and adult human B cell responses to rotavirus share common immunodominant variable gene repertoires. J Immunol, 171(9), 4680-8, 2003.
• Venter, Marietjie, Rock, Michael, Puren, Adrian J, Tiemessen, Caroline T, Crowe, James E Respiratory syncytial virus nucleoprotein-specific cytotoxic T-cell epitopes in a South African population of diverse HLA types are conserved in circulating field strains. J Virol, 77(13), 7319-29, 2003.
• Peters, Timothy R, Jennings, Mark T, Crowe, James E Pneumocystis carinii pneumonia in a girl with a midbrain glioma. Pediatr Hematol Oncol, 19(2), 141-3, 2002.
• Brock, Sean C, McGraw, Patricia A, Wright, Peter F, Crowe, James E The human polymeric immunoglobulin receptor facilitates invasion of epithelial cells by Streptococcus pneumoniae in a strain-specific and cell type-specific manner. Infect Immun, 705091-5, 2002.
• Weitkamp, J H, Crowe, J E Blood donor leukocyte reduction filters as a source of human B lymphocytes. Biotechniques, 31(3), 464, 466, 2001.
• Pierson, R N, Crowe, J E, Pfeiffer, S, Atkinson, J, Azimzadeh, A, Miller, G G CD40-ligand in primate cardiac allograft and viral immunity. Immunol Res, 23(2-3), 253-62, 2001.
• Crowe, J E, Suara, R O, Brock, S, Kallewaard, N, House, F, Weitkamp, J H Genetic and structural determinants of virus neutralizing antibodies. Immunol Res, 23(2-3), 135-45, 2001.
• Crowe, J E Influence of maternal antibodies on neonatal immunization against respiratory viruses. Clin Infect Dis, 33(10), 1720-7, 2001.
• Crowe, J E, Firestone, C Y, Murphy, B R Passively acquired antibodies suppress humoral but not cell-mediated immunity in mice immunized with live attenuated respiratory syncytial virus vaccines. J Immunol, 167(7), 3910-8, 2001.
• Crowe, J E Respiratory syncytial virus vaccine development. Vaccine, 20 Suppl 1S32-7, 2001.
• Pastey, M K, Gower, T L, Spearman, P W, Crowe, J E, Graham, B S A RhoA-derived peptide inhibits syncytium formation induced by respiratory syncytial virus and parainfluenza virus type 3. Nat Med, 6(1), 35-40, 2000.
• Weitkamp J-H, Crowe JE Jr "Current approaches to vaccine development against disease caused by respiratory syncytial virus (RSV)." Monatsschr Kinderheilkd, 148980-989, 2000.
• Wright, P F, Karron, R A, Belshe, R B, Thompson, J, Crowe, J E, Boyce, T G, Halburnt, L L, Reed, G W, Whitehead, S S, Anderson, E L, Wittek, A E, Casey, R, Eichelberger, M, Thumar, B, Randolph, V B, Udem, S A, Chanock, R M, Murphy, B R Evaluation of a live, cold-passaged, temperature-sensitive, respiratory syncytial virus vaccine candidate in infancy. J Infect Dis, 182(5), 1331-42, 2000.
• Weitkamp, J H, Tang, Y W, Haas, D W, Midha, N K, Crowe, J E Recurrent Achromobacter xylosoxidans bacteremia associated with persistent lymph node infection in a patient with hyper-immunoglobulin M syndrome. Clin Infect Dis, 31(5), 1183-7, 2000.
• Peters, T R, Brumbaugh, D E, Lawton, A R, Crowe, J E Recurrent pneumococcal arthritis as the presenting manifestation of X-linked agammaglobulinemia. Clin Infect Dis, 31(5), 1287-8, 2000.
• Whitehead, S S, Firestone, C Y, Karron, R A, Crowe, J E, Elkins, W R, Collins, P L, Murphy, B R Addition of a missense mutation present in the L gene of respiratory syncytial virus (RSV) cpts530/1030 to RSV vaccine candidate cpts248/404 increases its attenuation and temperature sensitivity. J Virol, 73(2), 871-7, 1999.
• Crowe, J E Host responses to respiratory virus infection and immunization. Curr Top Microbiol Immunol, 236191-214, 1999.
• Sakurai, H, Williamson, R A, Crowe, J E, Beeler, J A, Poignard, P, Bastidas, R B, Chanock, R M, Burton, D R Human antibody responses to mature and immature forms of viral envelope in respiratory syncytial virus infection: significance for subunit vaccines. J Virol, 73(4), 2956-62, 1999.
• Fisher, R G, Crowe, J E, Johnson, T R, Tang, Y W, Graham, B S Passive IgA monoclonal antibody is no more effective than IgG at protecting mice from mucosal challenge with respiratory syncytial virus. J Infect Dis, 180(4), 1324-7, 1999.
• Pastey, M K, Crowe, J E, Graham, B S RhoA interacts with the fusion glycoprotein of respiratory syncytial virus and facilitates virus-induced syncytium formation. J Virol, 73(9), 7262-70, 1999.
• Crowe JE Jr, Randolph V, Murphy BR "The live attenuated subgroup B respiratory syncytial virus vaccine candidate RSV 2B33F is attenuated and immunogenic in chimpanzees, but exhibits partial loss of the ts phenotype following replication in vivo." Virus Research, 5913-22, 1999.
• Crowe, J E, Randolph, V, Murphy, B R The live attenuated subgroup B respiratory syncytial virus vaccine candidate RSV 2B33F is attenuated and immunogenic in chimpanzees, but exhibits partial loss of the ts phenotype following replication in vivo. Virus Res, 59(1), 13-22, 1999.
• Crowe, J E Immune responses of infants to infection with respiratory viruses and live attenuated respiratory virus candidate vaccines. Vaccine, 16(14-15), 1423-32, 1998.
• Crowe, J E, Gilmour, P S, Murphy, B R, Chanock, R M, Duan, L, Pomerantz, R J, Pilkington, G R Isolation of a second recombinant human respiratory syncytial virus monoclonal antibody fragment (Fab RSVF2-5) that exhibits therapeutic efficacy in vivo. J Infect Dis, 177(4), 1073-6, 1998.
• Crowe, J E, Firestone, C Y, Crim, R, Beeler, J A, Coelingh, K L, Barbas, C F, Burton, D R, Chanock, R M, Murphy, B R Monoclonal antibody-resistant mutants selected with a respiratory syncytial virus-neutralizing human antibody fab fragment (Fab 19) define a unique epitope on the fusion (F) glycoprotein. Virology, 252(2), 373-5, 1998.
• Karron, R A, Wright, P F, Crowe, J E, Clements-Mann, M L, Thompson, J, Makhene, M, Casey, R, Murphy, B R Evaluation of two live, cold-passaged, temperature-sensitive respiratory syncytial virus vaccines in chimpanzees and in human adults, infants, and children. J Infect Dis, 176(6), 1428-36, 1997.
• Juhasz, K, Whitehead, S S, Bui, P T, Biggs, J M, Crowe, J E, Boulanger, C A, Collins, P L, Murphy, B R The temperature-sensitive (ts) phenotype of a cold-passaged (cp) live attenuated respiratory syncytial virus vaccine candidate, designated cpts530, results from a single amino acid substitution in the L protein. J Virol, 71(8), 5814-9, 1997.
• Crowe JE Jr, Murphy BR "Vaccines against respiratory syncytial virus and parainfluenza type 3." New Generation Vaccines, 2nd ed, Levine MM, Woodrow GC, Kaper JB, Cobon GS, 711-725, 1997.
• Crowe, J E, Firestone, C Y, Whitehead, S S, Collins, P L, Murphy, B R Acquisition of the ts phenotype by a chemically mutagenized cold-passaged human respiratory syncytial virus vaccine candidate results from the acquisition of a single mutation in the polymerase (L) gene. Virus Genes, 13(3), 269-73, 1996.
• Crowe, J E, Bui, P T, Firestone, C Y, Connors, M, Elkins, W R, Chanock, R M, Murphy, B R Live subgroup B respiratory syncytial virus vaccines that are attenuated, genetically stable, and immunogenic in rodents and nonhuman primates. J Infect Dis, 173(4), 829-39, 1996.
• Firestone, C Y, Whitehead, S S, Collins, P L, Murphy, B R, Crowe, J E Nucleotide sequence analysis of the respiratory syncytial virus subgroup A cold-passaged (cp) temperature sensitive (ts) cpts-248/404 live attenuated virus vaccine candidate. Virology, 225(2), 419-22, 1996.
• Crowe JE Jr "The role of antibodies in respiratory viral immunity." Seminars in Virology, 7273-283, 1996.
• Connors, M, Crowe, J E, Firestone, C Y, Murphy, B R, Collins, P L A cold-passaged, attenuated strain of human respiratory syncytial virus contains mutations in the F and L genes. Virology, 208(2), 478-84, 1995.
• Crowe, J E, Bui, P T, Siber, G R, Elkins, W R, Chanock, R M, Murphy, B R Cold-passaged, temperature-sensitive mutants of human respiratory syncytial virus (RSV) are highly attenuated, immunogenic, and protective in seronegative chimpanzees, even when RSV antibodies are infused shortly before immunization. Vaccine, 13(9), 847-55, 1995.
• Crowe, J E Current approaches to the development of vaccines against disease caused by respiratory syncytial virus (RSV) and parainfluenza virus (PIV). A meeting report of the WHO Programme for Vaccine Development. Vaccine, 13(4), 415-21, 1995.
• Kulkarni, A B, Collins, P L, Bacik, I, Yewdell, J W, Bennink, J R, Crowe, J E, Murphy, B R Cytotoxic T cells specific for a single peptide on the M2 protein of respiratory syncytial virus are the sole mediators of resistance induced by immunization with M2 encoded by a recombinant vaccinia virus. J Virol, 69(2), 1261-4, 1995.
• Hsu, K H, Crowe, J E, Lubeck, M D, Davis, A R, Hung, P P, Chanock, R M, Murphy, B R Isolation and characterization of a highly attenuated respiratory syncytial virus (RSV) vaccine candidate by mutagenesis of the incompletely attenuated RSV A2 ts-1 NG-1 mutant virus. Vaccine, 13(5), 509-15, 1995.
• Crowe, J E, Bui, P T, Davis, A R, Chanock, R M, Murphy, B R A further attenuated derivative of a cold-passaged temperature-sensitive mutant of human respiratory syncytial virus retains immunogenicity and protective efficacy against wild-type challenge in seronegative chimpanzees. Vaccine, 12(9), 783-90, 1994.
• Murphy, B R, Hall, S L, Kulkarni, A B, Crowe, J E, Collins, P L, Connors, M, Karron, R A, Chanock, R M An update on approaches to the development of respiratory syncytial virus (RSV) and parainfluenza virus type 3 (PIV3) vaccines. Virus Res, 32(1), 13-36, 1994.
• Crowe JE Jr, Murphy BR, Chanock RM, Williamson A, Barbas CF III, Burton DR "Human RSV monoclonal antibody Fab cloned from a combinatorial library and produced in E. coli is effective therapeutically when introduced directly into the lungs of RSV-infected mice." Brown F, Chanock RM, Ginsberg HS, Norrby E, eds. Vaccines 94: Modern Approaches to New Vaccines Including Prevention of AIDS. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 315-320, 1994.
• Crowe, J E, Cheung, P Y, Wallace, E F, Chanock, R M, Larrick, J W, Murphy, B R, Fry, K Isolation and characterization of a chimpanzee monoclonal antibody to the G glycoprotein of human respiratory syncytial virus. Clin Diagn Lab Immunol, 1(6), 701-6, 1994.
• Murphy BR, Crowe JE Jr, Lubeck MD, Hsu K-HL, Hall SL, Karron RA, Clements ML, Wright PF, Belshe RB, Chanock RM "Live attenuated vaccines for respiratory syncytial virus (RSV) and parainfluenza virus type 3 (PIV 3)." Report of the 104th Ross Conference on Pediatric Research, published by Ross Products, 173-178, 1994.
• Crowe, J E, Murphy, B R, Chanock, R M, Williamson, R A, Barbas, C F, Burton, D R Recombinant human respiratory syncytial virus (RSV) monoclonal antibody Fab is effective therapeutically when introduced directly into the lungs of RSV-infected mice. Proc Natl Acad Sci U S A, 91(4), 1386-90, 1994.
• Crowe, J E, Bui, P T, London, W T, Davis, A R, Hung, P P, Chanock, R M, Murphy, B R Satisfactorily attenuated and protective mutants derived from a partially attenuated cold-passaged respiratory syncytial virus mutant by introduction of additional attenuating mutations during chemical mutagenesis. Vaccine, 12(8), 691-9, 1994.
• Crowe, J E, Collins, P L, London, W T, Chanock, R M, Murphy, B R A comparison in chimpanzees of the immunogenicity and efficacy of live attenuated respiratory syncytial virus (RSV) temperature-sensitive mutant vaccines and vaccinia virus recombinants that express the surface glycoproteins of RSV. Vaccine, 11(14), 1395-404, 1993.
• Crowe JE Jr, Barbas CF III, Bui P, Cababa D, Jones TM, Zebedee SL, Murphy BR, Chanock RM, Burton DR "Human monoclonal Fab fragments derived from a combinatorial library bind to respiratory syncytial virus F glycoprotein and neutralize infectivity." Brown F, Chanock RM, Ginsberg HS, Lerner RA, eds. Vaccines 93: Modern Approaches to New Vaccines Including Prevention of AIDS. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, (), 7-11, 1993.
• Chanock, R M, Crowe, J E, Murphy, B R, Burton, D R Human monoclonal antibody Fab fragments cloned from combinatorial libraries: potential usefulness in prevention and/or treatment of major human viral diseases. Infect Agents Dis, 2(3), 118-31, 1993.
• Barbas, C F, Crowe, J E, Cababa, D, Jones, T M, Zebedee, S L, Murphy, B R, Chanock, R M, Burton, D R Human monoclonal Fab fragments derived from a combinatorial library bind to respiratory syncytial virus F glycoprotein and neutralize infectivity. Proc Natl Acad Sci U S A, 89(21), 10164-8, 1992.
• Murphy BR, Hall SL, Crowe JE Jr, Collins PL, Subbarao EK, Connors M, London WT, Chanock RM "The use of chimpanzees in respiratory virus research." Erwin J, Landon JC, eds. Chimpanzee Conservation and Public Health: Environments for the Future, 21 28, 1992.

Education

• M.D. - University of North Carolina at Chapel Hill, 1987
• Fellowship - National Institutes of Health, 1990
• Residency - University of North Carolina Health Care System, 1990
• Internship - University of North Carolina Health Care System, 1988