Vanderbilt-Ingram Cancer Center

The VICC.ORG Investigator Directory

Kathleen L. Gould, Ph.D.

Professor of Cell and Developmental Biology
HHMI Investigator
VICC Member
Researcher

Contact Information:

Vanderbilt University Medical Center
B-2309 Medical Center North
Nashville, TN 37232-2175
615-343-9502

Research Specialty:

Cell cycle control

Research Description:

Proper coordination of nuclear and cell division is necessary for the normal development of all eukaryotic organisms and for the maintenance of genomic integrity. The goals of my laboratory are to understand the molecular mechanisms regulating cytokinesis and also to learn how cytokinesis is coordinated with other events of the cell cycle such as chromosome segregation and microtubule cytoskeletal rearrangements. We are asking 1) what is the full complement of proteins that comprise the cytokinetic ring, 2) how is the cytokinetic ring precisely localized between segregating chromosomes, 3) what molecular event(s) triggers constriction of the cytokinetic ring, and 4) what ensures the correct timing of ring constriction with respect to chromosome segregation?

To address these questions expeditiously, we are using fission yeast as a model organism.

Fission yeast offers many experimental advantages for the study of cell cycle regulation including facile genetics and a fully sequenced and annotated genome. Using genetics, proteomics, live cell imaging, biochemistry, and structural biology we are unraveling the sequence of events that directs formation and then constriction of the cytokinetic ring. We are also studying protein phosphorylation signaling pathways and ubiquitin ligases that control the spatial precision of cytokinesis, the organization of the microtubule cytoskeleton through the cell cycle, and the timing of cytokinetic events during mitosis.

Publications:

• Chen, JS, Broadus, MR, McLean, JR, Feoktistova, A, Ren, L, Gould, KL Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast Clp1/Cdc14 phosphatase. Mol Cell Proteomics, 2013.
• Ma H, McLean JR, Chao LF, Mana-Capelli S, Paramasivam M, Hagstrom KA, Gould KL, McCollum D. A highly efficient multifunctional tandem affinity purification approachapplicable to diverse organisms. Mol Cell Proteomics, 11(8), 501-11, 2012.
• Bohnert, KA, Gould, KL Cytokinesis-based constraints on polarized cell growth in fission yeast. PLoS Genet, 8(10), e1003004, 2012.
• Wang, Y, Li, WZ, Johnson, AE, Luo, ZQ, Sun, L, Feoktistova, A, McDonald, WH, McLeod, I, Yates, III, JR, Gould, KL, McCollum, D, and Jin, QW Dnt1 Acts as a Mitotic Inhibitor of the Spindle Checkpoint Protein Dma1 in Fission Yeast. Mol Biol Cell, 23(17), 3338-56, 2012.
• Johnson, AE, Collier, SE, Ohi, MD, Gould, KL Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic checkpoint function. J Biol Chem, 287(31), 25741-8, 2012.
• Clement, A, Solnica-Krezel, L, Gould, KL Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis. Dev Dyn, 2012.
• Broadus, MR, Gould, KL Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress. Mol Biol Cell, 23(20), 4118-28, 2012.
• Lu, LX, Domingo-Sananes, MR, Huzarska, M, Novak, B, Gould, KL Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches. Proc Natl Acad Sci U S A, 109(25), 9899-904, 2012.
• Johnson, AE, McCollum, D, Gould, KL Polar opposites: Fine-tuning cytokinesis through SIN asymmetry. Cytoskeleton (Hoboken), 69(10), 686-99, 2012.
• Pancaldi V, Saraç OS, Rallis C, McLean JR, PÅ™evorovský M, Gould K, Beyer A, Bähler J. Predicting the fission yeast protein interaction network. G3: Genes, Genomes and Genetics, 2(4), 453-67, 2012.
• Feoktistova, A, Morrell-Falvey, J, Chen, JS, Singh, NS, Balasubramanian, MK, Gould, KL The fission yeast SIN kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11. Mol Biol Cell, 23(9), 1636-45, 2012.
• Chen, JS, Lu, LX, Ohi, MD, Creamer, KM, English, C, Partridge, JF, Ohi, R, Gould, KL Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol, 195(4), 583-93, 2011.
• Johnson, AE, Gould, KL Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase. EMBO J, 30(2), 341-54, 2011.
• Peckys, DB, Mazur, P, Gould, KL, de Jonge, N Fully hydrated yeast cells imaged with electron microscopy. Biophys J, 100(10), 2522-9, 2011.
• Goettel, JA, Liang, D, Hilliard, VC, Edelblum, KL, Broadus, MR, Gould, KL, Hanks, SK, Polk, DB KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation of MEK1. Exp Cell Res, 317(4), 452-63, 2011.
• Prevorovsky, M, Atkinson, SR, Ptackova, M, McLean, JR, Gould, KL, Folk, P, Puta, F, and Bahler, J N-termini of fungal CSL transcription factors are dirordered, enriched in regulatory motifs and inhibit DNA binding in fission yeast.. PLoS ONE, 6e23650, 2011.
• Bohnert, KA, Gould, KL On the cutting edge: post-translational modifications in cytokinesis. Trends Cell Biol, 21(5), 283-92, 2011.
• Singh NS, Shao N, McLean JR, Sevugan M, Ren L, Chew TG, Bimbo A, Sharma R, Tang X, Gould KL, Balasubramanian MK. SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast. Curr Biol, 21(23), 1968-78, 2011.
• McLean, JR, Chaix, D, Ohi, MD, Gould, KL State of the APC/C: organization, function, and structure. Crit Rev Biochem Mol Biol, 46(2), 118-36, 2011.
• McLean, JR, Kouranti, I, Gould, KL Survey of the phosphorylation status of the Schizosaccharomyces pombe deubiquitinating enzyme (DUB) family. J Proteome Res, 10(3), 1208-15, 2011.
• Ren, L, McLean, JR, Hazbun, TR, Fields, S, Vander Kooi, C, Ohi, MD, Gould, KL Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. PLoS One, 6(2), e16719, 2011.
• Almonacid M, Celton-Morizur S, Jakubowski JL, Dingli F, Loew D, Mayeux A, Chen JS, Gould KL, Clifford DM, Paoletti A. Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin.. Cur Biol, 21(6), 473-9, 2011.
• Clément, A, Solnica-Krezel, L, Gould, KL The Cdc14B phosphatase contributes to ciliogenesis in zebrafish. Development, 138(2), 291-302, 2011.
• Kouranti, I, McLean, JR, Feoktistova, A, Liang, P, Johnson, AE, Roberts-Galbraith, RH, Gould, KL A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity. PLoS Biol, 8(9), 2010.
• Roberts-Galbraith, RH, Ohi, MD, Ballif, BA, Chen, JS, McLeod, I, McDonald, WH, Gygi, SP, Yates, JR, Gould, KL Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site. Mol Cell, 39(1), 86-99, 2010.
• Papadopoulou K, Chen JS, Mead E, Feoktistova A, Petit C, Agarwal M, Jamal M, Malik A, Spanos A, Sedgwick SG, Karagiannis J, Balasubramanian MK, Gould KL, McInerny CJ. Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p.. J Cell Sci, 123(Pt 24), 4374-81, 2010.
• Roberts-Galbraith, RH, Gould, KL Setting the F-BAR: functions and regulation of the F-BAR protein family. Cell Cycle, 9(20), 4091-7, 2010.
• Vander Kooi, CW, Ren, L, Xu, P, Ohi, MD, Gould, KL, Chazin, WJ The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure, 18(5), 584-93, 2010.
• Bohnert, KA, Chen, JS, Clifford, DM, Vander Kooi, CW, Gould, KL A Link between Aurora Kinase and Clp1/Cdc14 Regulation Uncovered by the Identification of a Fission Yeast Borealin-like Protein. Mol Biol Cell, 2009.
• Roberts-Galbraith RH, Chen JS, Wang J, Gould KL. The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring. J Cell Biol, 184(1)113-27, 2009.
• Roberts-Galbraith, RH, Gould, KL Stepping into the ring: the SIN takes on contractile ring assembly. Genes Dev, 22(22), 3082-8, 2008.
• Clifford, DM, Wolfe, BA, Roberts-Galbraith, RH, McDonald, WH, Yates, JR, Gould, KL The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1. J Cell Biol, 181(1), 79-88, 2008.
• Chen CT, Feoktistova A, Chen JS, Shim YS, Clifford DM, Gould KL, McCollum D. The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1.. Curr Biol, 18(20), 1594-9, 2008.
• Clifford, DM, Chen, CT, Roberts, RH, Feoktistova, A, Wolfe, BA, Chen, JS, McCollum, D, Gould, KL The role of Cdc14 phosphatases in the control of cell division. Biochem Soc Trans, 36(Pt 3), 436-8, 2008.
• Sczaniecka M, Feoktistova A, May KM, Chen JS, Blyth J, Gould KL, Hardwick KG. The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).. J Biol Chem, 283(34)23039-47, 2008.
• Ohi, M.D., Feoktistova, A. Ren, L., Yip, C., Cheng, Y., Chen, J.-S., Yoon, H.-J., Wall, J.S., Huang, Z., Penczek, P.A., Gould, K.L.*, and Walz, T.* Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1.. Mol Cell, 28871-885, 2007, *co-corresponding authors.
• Ohi, MD, Ren, L, Wall, JS, Gould, KL, Walz, T Structural characterization of the fission yeast U5.U2/U6 spliceosome complex. Proc Natl Acad Sci U S A, 104(9), 3195-200, 2007.
• Mehta, S, Gould, KL Identification of functional domains within the SIN kinase Cdc7. J Biol Chem, 281(15), 9935-41, 2006.
• Wolfe, BA, McDonald, WH, Yates, JR, Gould, KL Phospho-Regulation of the Cdc14/Clp1 Phosphatase Delays Late Mitotic Events in S. pombe. Dev Cell, 11(3), 423-30, 2006.
• Rosenberg, JA, Tomlin, GC, McDonald, WH, Snydsman, BE, Muller, EG, Yates, JR, Gould, KL Ppc89 links multiple proteins, including the septation initiation network, to the core of the fission yeast spindle-pole body. Mol Biol Cell, 17(9), 3793-805, 2006.
• Yoon, HJ, Feoktistova, A, Chen, JS, Jennings, JL, Link, AJ, Gould, KL Role of HCN1 and its phosphorylation in fission yeast APC/C function. J Biol Chem, 28(43), 32284-93, 2006.
• Vander Kooi, CW, Ohi, MD, Rosenberg, JA, Oldham, ML, Newcomer, ME, Gould, KL, Chazin, WJ The Prp19 U-box Crystal Structure Suggests a Common Dimeric Architecture for a Class of Oligomeric E3 Ubiquitin Ligases(,). Biochemistry, 45(1), 121-130, 2006.
• Alonso-Nuñez, ML, An, H, Martín-Cuadrado, AB, Mehta, S, Petit, C, Sipiczki, M, Del Rey, F, Gould, KL, Vázquez de Aldana, CR Ace2p Controls the Expression of Genes Required for Cell Separation in Schizosaccharomyces pombe. Mol Biol Cell, 162003-17, 2005.
• Petit, CS, Mehta, S, Roberts, RH, Gould, KL Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression. J Cell Sci, 118(Pt 24), 5731-42, 2005.
• Ren, L, Feoktistova, A, McDonald, WH, Den Haese, G, Morrell, JL, Gould, KL Analysis of the role of phosphorylation in fission yeast Cdc13/cyclin B function. J Biol Chem, 28014591-96, 2005.
• Morrell-Falvey, JL, Ren, L, Feoktistova, A, Haese, GD, Gould, KL Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p. J Cell Sci, 118(Pt 23), 5563-73, 2005.
• Carnahan, RH, Feoktistova, A, Ren, L, Niessen, S, Yates, JR, Gould, KL Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex. Eukaryot Cell, 4(3), 577-87, 2005.
• Venkatram, S, Jennings, JL, Link, A, Gould, KL Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring. Mol Biol Cell, 16(6), 3052-63, 2005.
• Martín-Cuadrado, AB, Morrell, JL, Konomi, M, An, H, Petit, C, Osumi, M, Balasubramanian, M, Gould, KL, Del Rey, F, de Aldana, CR Role of septins and the exocyst complex in the function of hydrolytic enzymes responsible for fission yeast cell separation. Mol Biol Cell, 16(10), 4867-81, 2005.
• Wolfe, BA, Gould, KL Split decisions: coordinating cytokinesis in yeast. Trends Cell Biol, 15(1), 10-18, 2005.
• Ohi, MD, Vander Kooi, CW, Rosenberg, JA, Ren, L, Hirsch, JP, Chazin, WJ, Walz, T, Gould, KL Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol Cell Biol, 25(1), 451-60, 2005.
• Wolfe, Benjamin A, Gould, Kathleen L Fission yeast Clp1p phosphatase affects G(2)/M transition and mitotic exit through Cdc25p inactivation. EMBO J, 23(4), 919-29, 2004.
• Venkatram, Srinivas, Tasto, Joseph J., Feoktistova, Anna, Jennings, Jennifer L., Link, Andrew J., Gould, Kathleen L. Identification and characterization of two novel proteins affecting fission yeast {gamma}-tubulin complex function. Mol Biol Cell, 15(5), 2287-301, 2004.
• Wolfe, Benjamin A, Gould, Kathleen L Inactivating cdc25, mitotic style. Cell Cycle, 3(5), 601-3, 2004.
• Gould, Kathleen L Protocols for experimentation with Schizosaccharomyces pombe. Methods, 33(3), 187-8, 2004.
• An, Hanbing, Morrell, Jennifer L, Jennings, Jennifer L, Link, Andrew J, Gould, Kathleen L Requirements of fission yeast septins for complex formation, localization, and function. Mol Biol Cell, 15(12), 5551-64, 2004.
• Morrell, JL, Tomlin, GC, Rajagopalan, S, Venkatram, S, Feoktistova, AS, Tasto, JJ, Mehta, S, Jennings, JL, Link, A, Balasubramanian, MK, Gould, KL Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB. Curr Biol, 14(7), 579-84, 2004.
• Gould, Kathleen L, Ren, Liping, Feoktistova, Anna S, Jennings, Jennifer L, Link, Andrew J Tandem affinity purification and identification of protein complex components. Methods, 33(3), 239-44, 2004.
• Morrell, Jennifer L, Nichols, Connie B, Gould, Kathleen L The GIN4 family kinase, Cdr2p, acts independently of septins in fission yeast. J Cell Sci, 117(Pt 22), 5293-302, 2004.
• Tasto, Joseph J, Morrell, Jennifer L, Gould, Kathleen L An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation. J Cell Biol, 1601093-103, 2003.
• Ohi, Melanie D, Vander Kooi, Craig W, Rosenberg, Joshua A, Chazin, Walter J, Gould, Kathleen L Structural insights into the U-box, a domain associated with multi-ubiquitination. Nat Struct Biol, 10250-5, 2003.
• Zhang, YZ, Cheng, H, Gould, KL, Golemis, EA, Roder, H Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, and mutational analysis. Biochemistry, 42(32), 9609-18, 2003.
• Carnahan, Robert H, Gould, Kathleen L The PCH family protein, Cdc15p, recruits two F-actin nucleation pathways to coordinate cytokinetic actin ring formation in Schizosaccharomyces pombe. J Cell Biol, 162(5), 851-62, 2003.
• Ohi, Melanie D, Gould, Kathleen L Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA, 8(6), 798-815, 2002.
• Guertin, D.A., Venkatram, S., Gould, K.L., and McCollum, D. . Dma1 prevents mitotic exit and cytokinesis by inhibiting the Septation Initiation Network (SIN). Dev. Cell, 3(8), 779-790, 2002.
• Guertin, DA, Venkatram, S, Gould, KL, McCollum, D Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN). Dev Cell, 3(6), 779-90, 2002.
• Yoon, Hyun-Joo, Feoktistova, Anna, Wolfe, Benjamin A, Jennings, Jennifer L, Link, Andrew J, Gould, Kathleen L Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr Biol, 12(23), 2048-54, 2002.
• Ohi, Melanie D, Link, Andrew J, Ren, Liping, Jennings, Jennifer L, McDonald, W Hayes, Gould, Kathleen L Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol, 22(7), 2011-24, 2002.
• Burns, C Geoffrey, Ohi, Ryoma, Mehta, Sapna, O'Toole, Eileen T, Winey, Mark, Clark, Tyson A, Sugnet, Charles W, Ares, Manuel, Gould, Kathleen L Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol, 22(3), 801-15, 2002.
• Schadick, K, Fourcade, HM, Boumenot, P, Seitz, JJ, Morrell, JL, Chang, L, Gould, KL, Partridge, JF, Allshire, RC, Kitagawa, K, Hieter, P, Hoffman, CS Schizosaccharomyces pombe Git7p, a member of the Saccharomyces cerevisiae Sgtlp family, is required for glucose and cyclic AMP signaling, cell wall integrity, and septation. Eukaryot Cell, 1(4), 558-67, 2002.
• MacCoss, MJ, McDonald, WH, Saraf, A, Sadygov, R, Clark, JM, Tasto, JJ, Gould, KL, Wolters, D, Washburn, M, Weiss, A, Clark, JI, Yates, JR Shotgun identification of protein modifications from protein complexes and lens tissue. Proc Natl Acad Sci U S A, 99(12), 7900-5, 2002.
• Tomlin, Gregory C, Morrell, Jennifer L, Gould, Kathleen L The spindle pole body protein Cdc11p links Sid4p to the fission yeast septation initiation network. Mol Biol Cell, 131203-14, 2002.
• Ochotorena, IL, Hirata, D, Kominami, K, Potashkin, J, Sahin, F, Wentz-Hunter, K, Gould, KL, Sato, K, Yoshida, Y, Vardy, L, Toda, T Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation. J Cell Sci, 114(Pt 16), 2911-20, 2001.
• Feoktistova, A, Magnelli, P, Abeijon, C, Perez, P, Lester, R L, Dickson, R C, Gould, K L Coordination between fission yeast glucan formation and growth requires a sphingolipase activity. Genetics, 158(4), 1397-411, 2001.
• Trautmann, S, Wolfe, BA, Jorgensen, P, Tyers, M, Gould, KL, McCollum, D Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression. Curr Biol, 11(12), 931-40, 2001.
• Chang, L, Morrell, J L, Feoktistova, A, Gould, K L Study of cyclin proteolysis in anaphase-promoting complex (APC) mutant cells reveals the requirement for APC function in the final steps of the fission yeast septation initiation network. Mol Cell Biol, 21(19), 6681-94, 2001.
• McCollum, D., and Gould, K.L Timing is everything: regulation of mitotic exit and cytokinesis by the MEN and SIN. Trends Cell Biol., 1189-95, 2001.
• McCollum, D, Gould, KL Timing is everything: regulation of mitotic exit and cytokinesis by the MEN and SIN. Trends Cell Biol, 11(2), 89-95, 2001.
• Bishop, DT, McDonald, WH, Gould, KL, Forsburg, SL Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis. Nucleic Acids Res, 28(11), 2214-20, 2000.
• Guertin, DA, Chang, L, Irshad, F, Gould, KL, McCollum, D The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast. EMBO J, 19(8), 1803-15, 2000.
• Behlke, MA, Dames, SA, McDonald, WH, Gould, KL, Devor, EJ, Walder, JA Use of high specific activity StarFire oligonucleotide probes to visualize low-abundance pre-mRNA splicing intermediates in S. pombe. Biotechniques, 29(4), 892-7, 2000.
• Morrell, J L, Morphew, M, Gould, K L A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast. Mol Biol Cell, 10(12), 4201-15, 1999.
• Burns, C G, Gould, K L Connections between pre-mRNA processing and regulation of the eukaryotic cell cycle. Front Horm Res, 2559-82, 1999.
• Naqvi, NI, Eng, K, Gould, KL, Balasubramanian, MK Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast. EMBO J, 18(4), 854-62, 1999.
• Burns, C G, Ohi, R, Krainer, A R, Gould, K L Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Proc Natl Acad Sci U S A, 96(24), 13789-94, 1999.
• McDonald, W H, Ohi, R, Smelkova, N, Frendewey, D, Gould, K L Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing. Mol Cell Biol, 19(8), 5352-62, 1999.
• McCollum, D, Feoktistova, A, Gould, K L Phosphorylation of the myosin-II light chain does not regulate the timing of cytokinesis in fission yeast. J Biol Chem, 274(25), 17691-5, 1999.
• Ohi, R, Gould, K L Regulating the onset of mitosis. Curr Opin Cell Biol, 11(2), 267-73, 1999.
• Machesky, LM, Gould, KL The Arp2/3 complex: a multifunctional actin organizer. Curr Opin Cell Biol, 11(1), 117-21, 1999.
• Zhang, YZ, Gould, KL, Dunbrack RL, JR, Cheng, H, Roder, H, Golemis, EA The evolutionarily conserved Dim1 protein defines a novel branch of the thioredoxin fold superfamily. Physiol Genomics, 1(3), 109-18, 1999.
• Berry, L D, Feoktistova, A, Wright, M D, Gould, K L The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function. Mol Cell Biol, 19(4), 2535-46, 1999.
• Gould, K L, Feoktistova, A, Fleig, U A phosphorylation site mutant of Schizosaccharomyces pombe cdc2p fails to promote the metaphase to anaphase transition. Mol Gen Genet, 259(4), 437-48, 1998.
• Gould, K L, Burns, C G, Feoktistova, A, Hu, C P, Pasion, S G, Forsburg, S L Fission yeast cdc24(+) encodes a novel replication factor required for chromosome integrity. Genetics, 149(3), 1221-33, 1998.
• Bähler, J, Steever, AB, Wheatley, S, Wang, Y, Pringle, JR, Gould, KL, McCollum, D Role of polo kinase and Mid1p in determining the site of cell division in fission yeast. J Cell Biol, 143(6), 1603-16, 1998.
• Breeding, C S, Hudson, J, Balasubramanian, M K, Hemmingsen, S M, Young, P G, Gould, K L The cdr2(+) gene encodes a regulator of G2/M progression and cytokinesis in Schizosaccharomyces pombe. Mol Biol Cell, 9(12), 3399-415, 1998.
• Plyte, SE, Feoktistova, A, Burke, JD, Woodgett, JR, Gould, KL Schizosaccharomyces pombe skp1+ encodes a protein kinase related to mammalian glycogen synthase kinase 3 and complements a cdc14 cytokinesis mutant. Mol Cell Biol, 17(3), 1756, 1997.
• Plyte, SE, Feoktistova, A, Burke, JD, Woodgett, JR, Gould, KL Schizosaccharomyces pombe skp1+ encodes a protein kinase related to mammalian glycogen synthase kinase 3 and complements a cdc14 cytokinesis mutant. Mol Cell Biol, 16(1), 179-91, 1996.
• Fleig, UN, Gould, KL, Nurse, P A dominant negative allele of p34cdc2 shows altered phosphoamino acid content and sequesters p56cdc13 cyclin. Mol Cell Biol, 12(5), 2295-301, 1992.
• Enoch, T, Gould, KL, Nurse, P Mitotic checkpoint control in fission yeast. Cold Spring Harb Symp Quant Biol, 56409-16, 1991.
• Gould, KL, Moreno, S, Owen, DJ, Sazer, S, Nurse, P Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function. EMBO J, 10(11), 3297-309, 1991.
• Gould, KL, Moreno, S, Tonks, NK, Nurse, P Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase. Science, 250(4987), 1573-6, 1990.
• Gould, KL, Nurse, P Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. Nature, 342(6245), 39-45, 1989.
• Gould, KL, Bretscher, A, Esch, FS, Hunter, T cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. EMBO J, 8(13), 4133-42, 1989.
• Gould, KL, Hunter, T Platelet-derived growth factor induces multisite phosphorylation of pp60c-src and increases its protein-tyrosine kinase activity. Mol Cell Biol, 8(8), 3345-56, 1988.
• Creutz, CE, Zaks, WJ, Hamman, HC, Crane, S, Martin, WH, Gould, KL, Oddie, KM, Parsons, SJ Identification of chromaffin granule-binding proteins. Relationship of the chromobindins to calelectrin, synhibin, and the tyrosine kinase substrates p35 and p36. J Biol Chem, 262(4), 1860-8, 1987.
• Isacke, CM, Meisenhelder, J, Brown, KD, Gould, KL, Gould, SJ, Hunter, T Early phosphorylation events following the treatment of Swiss 3T3 cells with bombesin and the mammalian bombesin-related peptide, gastrin-releasing peptide. EMBO J, 5(11), 2889-98, 1986.
• Woodgett, JR, Gould, KL, Hunter, T Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements. Eur J Biochem, 161(1), 177-84, 1986.
• Gould, KL, Woodgett, JR, Isacke, CM, Hunter, T The protein-tyrosine kinase substrate p36 is also a substrate for protein kinase C in vitro and in vivo. Mol Cell Biol, 6(7), 2738-44, 1986.
• Gould, KL, Cooper, JA, Bretscher, A, Hunter, T The protein-tyrosine kinase substrate, p81, is homologous to a chicken microvillar core protein. J Cell Biol, 102(2), 660-9, 1986.
• Gould, KL, Woodgett, JR, Cooper, JA, Buss, JE, Shalloway, D, Hunter, T Protein kinase C phosphorylates pp60src at a novel site. Cell, 42(3), 849-57, 1985.
• Gould, KL, Cooper, JA, Hunter, T The 46,000-dalton tyrosine protein kinase substrate is widespread, whereas the 36,000-dalton substrate is only expressed at high levels in certain rodent tissues. J Cell Biol, 98(2), 487-97, 1984.