Vanderbilt-Ingram Cancer Center

The VICC.ORG Investigator Directory

Lawrence J. Marnett, Ph.D.

See also:

• A.B. Hancock Jr. Memorial Laboratory
• Genome Maintenance Research Program
• Vanderbilt Institute of Chemical Biology
• BRET: Biomedical Research Education & Training Faculty Listing
• PubMed Listing of Dr. Marnett's Publications

Director, Vanderbilt Institute of Chemical Biology
Mary Geddes Stahlman Professor of Cancer Research
Director, A.B. Hancock Jr. Memorial Laboratory
Director, Vanderbilt Institute of Chemical Biology
VICC Member
Researcher

Contact Information:

Vanderbilt University Medical Center
850-A Robinson Research Building
Nashville, TN 37232-0146
615-343-7329

Research Specialty:

Cyclooxygenase-2 structure and function; chemistry and biology of DNA damage-dependent mutagenesis and signaling

Research Description:

A major interest in the Marnett laboratory is the structure and function of cyclooxygenase-2 (COX-2). This enzyme catalyzes the committed step in the production of prostaglandins and is the molecular target for non-steroidal antiinflammatory drugs. Functional analysis of COX-2 by structure-guided mutagenesis has uncovered new strategies for synthesizing COX-2 inhibitors and has suggested new biological roles for the enzyme. Current work focuses on the generation of novel antiinflammatory drugs with additional pharmacological functions and on investigations of the chemistry and biology of COX-2-dependent oxygenation of the endocannabinoid, 2-arachidonylglycerol, to glyceryl prostaglandins.

Another major interest in the Marnett laboratory is the chemistry and biology of DNA damage by endogenously generated products of oxidative stress. Individual products of DNA damage are incorporated into viral genomes for in vivo mutagenesis experiments. Similar experiments are used to identify human DNA repair enzymes that remove specific forms of DNA damage.

A new area of interest in the laboratory is the investigation of protein targets of drugs and toxic compounds and the consequences of protein modification in modulating biological function.

A broad range of techniques are used by the Marnett group including chemical synthesis, enzymology, protein expression, site-directed mutagenesis, bioanalytical chemistry, mass spectrometry, in vivo mutagenesis, tissue culture, proteomics, and techniques of signal transduction.

Publications:

• Szekely, J, Wang, H, Peplowski, KM, Knutson, CG, Marnett, LJ, Rizzo, CJ "One-pot" syntheses of malondialdehyde adducts of nucleosides. Nucleosides Nucleotides Nucleic Acids, 27(2), 103-9, 2008.
• Bala, M, Chin, CN, Logan, AT, Amin, T, Marnett, LJ, Boutaud, O, Oates, JA Acetylation of prostaglandin H2 synthases by aspirin is inhibited by redox cycling of the peroxidase. Biochem Pharmacol, 75(7), 1472-81, 2008.
• Szekely, J, Rizzo, CJ, Marnett, LJ Chemical properties of oxopropenyl adducts of purine and pyrimidine nucleosides and their reactivity toward amino acid cross-link formation. J Am Chem Soc, 130(7), 2195-201, 2008.
• Vanderveen, LA, Harris, TM, Jen-Jacobson, L, Marnett, LJ Formation of DNA-Protein Cross-Links Between gamma-Hydroxypropanodeoxyguanosine and EcoRI. Chem Res Toxicol, 2008.
• Vila, A, Tallman, KA, Jacobs, AT, Liebler, DC, Porter, NA, Marnett, LJ Identification of protein targets of 4-hydroxynonenal using click chemistry for ex vivo biotinylation of azido and alkynyl derivatives. Chem Res Toxicol, 21(2), 432-44, 2008.
• Wang, Y, Musser, SK, Saleh, S, Marnett, LJ, Egli, M, Stone, MP Insertion of dNTPs opposite the 1,N2-propanodeoxyguanosine adduct by Sulfolobus solfataricus P2 DNA polymerase IV. Biochemistry, 47(28), 7322-34, 2008.
• Knutson, CG, Skipper, PL, Liberman, RG, Tannenbaum, SR, Marnett, LJ Monitoring in Vivo Metabolism and Elimination of the Endogenous DNA Adduct, M1dG {3-(2-Deoxy-beta-d-erythro-pentofuranosyl)pyrimido[1,2-alpha]purin-10(3H)-one}, by Accelerator Mass Spectrometry. Chem Res Toxicol, 2008.
• Rouzer, CA, Marnett, LJ Non-redundant functions of cyclooxygenases: oxygenation of endocannabinoids. J Biol Chem, 283(13), 8065-9, 2008.
• Turman, MV, Kingsley, PJ, Rouzer, CA, Cravatt, BF, Marnett, LJ Oxidative metabolism of a fatty acid amide hydrolase-regulated lipid, arachidonoyltaurine. Biochemistry, 47(12), 3917-25, 2008.
• Stafford, JB, Marnett, LJ Prostaglandin E2 inhibits tumor necrosis factor-alpha RNA through PKA type I. Biochem Biophys Res Commun, 366(1), 104-9, 2008.
• Felts, AS, Siegel, BS, Young, SM, Moth, CW, Lybrand, TP, Dannenberg, AJ, Marnett, LJ, Subbaramaiah, K Sulindac Derivatives That Activate the Peroxisome Proliferator-activated Receptor gamma but Lack Cyclooxygenase Inhibition. J Med Chem, 2008.
• Ghidu, VP, Wang, J, Wu, B, Liu, Q, Jacobs, A, Marnett, LJ, Sulikowski, GA Synthesis and evaluation of the cytotoxicity of apoptolidinones A and D. J Org Chem, 73(13), 4949-55, 2008.
• Wang, Y, Schnetz-Boutaud, NC, Saleh, S, Marnett, LJ, Stone, MP Bulge migration of the malondialdehyde OPdG DNA adduct when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene. Chem Res Toxicol, 20(8), 1200-10, 2007.
• Wang, H, Xie, H, Sun, X, Kingsley, PJ, Marnett, LJ, Cravatt, BF, Dey, SK Differential regulation of endocannabinoid synthesis and degradation in the uterus during embryo implantation. Prostaglandins Other Lipid Mediat, 83(1-2), 62-74, 2007.
• Jacobs, AT, Marnett, LJ Heat shock factor 1 attenuates 4-Hydroxynonenal-mediated apoptosis: critical role for heat shock protein 70 induction and stabilization of Bcl-XL. J Biol Chem, 282(46), 33412-20, 2007.
• Vila, A, Rosengarth, A, Piomelli, D, Cravatt, B, Marnett, LJ Hydrolysis of prostaglandin glycerol esters by the endocannabinoid-hydrolyzing enzymes, monoacylglycerol lipase and fatty acid amide hydrolase. Biochemistry, 46(33), 9578-85, 2007.
• Druckova, A, Mernaugh, RL, Ham, AJ, Marnett, LJ Identification of the protein targets of the reactive metabolite of teucrin A in vivo in the rat. Chem Res Toxicol, 20(10), 1393-408, 2007.
• Joo, M, Kwon, M, Sadikot, RT, Kingsley, PJ, Marnett, LJ, Blackwell, TS, Peebles, RS, Urade, Y, Christman, JW Induction and function of lipocalin prostaglandin D synthase in host immunity. J Immunol, 179(4), 2565-75, 2007.
• Kingsley, PJ, Marnett, LJ LC-MS-MS analysis of neutral eicosanoids. Methods Enzymol, 43391-112, 2007.
• Rouzer, CA, Ivanova, PT, Byrne, MO, Brown, HA, Marnett, LJ Lipid Profiling Reveals Glycerophospholipid Remodeling in Zymosan-Stimulated Macrophages. Biochemistry, 2007.
• Knutson, CG, Wang, H, Rizzo, CJ, Marnett, LJ Metabolism and elimination of the endogenous DNA adduct, 3-(2-deoxy-beta-D-erythropentofuranosyl)-pyrimido[1,2-alpha]purine-10(3H)-one, in the rat. J Biol Chem, 282(50), 36257-64, 2007.
• Knutson, CG, Akingbade, D, Crews, BC, Voehler, M, Stec, DF, Marnett, LJ Metabolism in vitro and in vivo of the DNA base adduct, M1G. Chem Res Toxicol, 20(3), 550-7, 2007.
• Blobaum, AL, Marnett, LJ Molecular determinants for the selective inhibition of Cyclooxygenase-2 by Lumiracoxib. J Biol Chem, 2007.
• Prusakiewicz, JJ, Turman, MV, Vila, A, Ball, HL, Al-Mestarihi, AH, Di Marzo, V, Marnett, LJ Oxidative metabolism of lipoamino acids and vanilloids by lipoxygenases and cyclooxygenases. Arch Biochem Biophys, 464(2), 260-8, 2007.
• Blobaum, AL, Marnett, LJ Structural and functional basis of cyclooxygenase inhibition. J Med Chem, 50(7), 1425-41, 2007.
• Muthian, G, Raikwar, HP, Johnson, C, Rajasingh, J, Kalgutkar, A, Marnett, LJ, Bright, JJ COX-2 Inhibitors Modulate IL-12 Signaling Through JAK-STAT Pathway Leading to Th1 Response in Experimental Allergic Encephalomyelitis. J Clin Immunol, 26(1), 73-85, 2006.
• Druckova, A, Marnett, LJ Characterization of the amino acid adducts of the enedial derivative of teucrin A. Chem Res Toxicol, 19(10), 1330-40, 2006.
• West, JD, Marnett, LJ Endogenous reactive intermediates as modulators of cell signaling and cell death. Chem Res Toxicol, 19(2), 173-94, 2006.
• Wang, H, Xie, H, Guo, Y, Zhang, H, Takahashi, T, Kingsley, PJ, Marnett, LJ, Das, SK, Cravatt, BF, Dey, SK Fatty acid amide hydrolase deficiency limits early pregnancy events. J Clin Invest, 116(8), 2122-31, 2006.
• Otteneder, MB, Knutson, CG, Daniels, JS, Hashim, M, Crews, BC, Remmel, RP, Wang, H, Rizzo, C, Marnett, LJ In vivo oxidative metabolism of a major peroxidation-derived DNA adduct, M1dG. Proc Natl Acad Sci U S A, 103(17), 6665-9, 2006.
• Rouzer, CA, Ivanova, PT, Byrne, MO, Milne, SB, Marnett, LJ, Brown, HA Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications. Biochemistry, 45(49), 14795-808, 2006.
• Wang, H, Kozekov, ID, Kozekova, A, Tamura, PJ, Marnett, LJ, Harris, TM, Rizzo, CJ Site-specific synthesis of oligonucleotides containing malondialdehyde adducts of deoxyguanosine and deoxyadenosine via a postsynthetic modification strategy. Chem Res Toxicol, 19(11), 1467-74, 2006.
• Rouzer, CA, Tranguch, S, Wang, H, Zhang, H, Dey, SK, Marnett, LJ Zymosan-induced glycerylprostaglandin and prostaglandin synthesis in resident peritoneal macrophages: roles of cyclo-oxygenase-1 and -2. Biochem J, 399(1), 91-9, 2006.
• West, JD, Marnett, LJ Alterations in gene expression induced by the lipid peroxidation product, 4-hydroxy-2-nonenal. Chem Res Toxicol, 18(11), 1642-53, 2005.
• VanderVeen, LA, Druckova, A, Riggins, JN, Sorrells, JL, Guengerich, FP, Marnett, LJ Differential DNA recognition and cleavage by EcoRI dependent on the dynamic equilibrium between the two forms of the malondialdehyde-deoxyguanosine adduct. Biochemistry, 44(13), 5024-33, 2005.
• Vélez-Cruz, R, Riggins, JN, Daniels, JS, Cai, H, Guengerich, FP, Marnett, LJ, Osheroff, N Exocyclic DNA lesions stimulate DNA cleavage mediated by human topoisomerase II alpha in vitro and in cultured cells. Biochemistry, 44(10), 3972-81, 2005.
• Rouzer, CA, Marnett, LJ Glycerylprostaglandin synthesis by resident peritoneal macrophages in response to a zymosan stimulus. J Biol Chem, 280(29), 26690-700, 2005.
• Kalgutkar, AS, Crews, BC, Saleh, S, Prudhomme, D, Marnett, LJ Indolyl esters and amides related to indomethacin are selective COX-2 inhibitors. Bioorg Med Chem, 13(24), 6810-22, 2005.
• Burleigh, ME, Babaev, VR, Patel, MB, Crews, BC, Remmel, RP, Morrow, JD, Oates, JA, Marnett, LJ, Fazio, S, Linton, MF Inhibition of cyclooxygenase with indomethacin phenethylamide reduces atherosclerosis in apoE-null mice. Biochem Pharmacol, 70(3), 334-42, 2005.
• West, JD, Ji, C, Marnett, LJ Modulation of DNA fragmentation factor 40 nuclease activity by poly(ADP-ribose) polymerase-1. J Biol Chem, 280(15), 15141-7, 2005.
• Guo, Y, Wang, H, Okamoto, Y, Ueda, N, Kingsley, PJ, Marnett, LJ, Schmid, HH, Das, SK, Dey, SK N-acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation. J Biol Chem, 280(25), 23429-32, 2005.
• Rouzer, CA, Jacobs, AT, Nirodi, CS, Kingsley, PJ, Morrow, JD, Marnett, LJ RAW264.7 cells lack prostaglandin-dependent autoregulation of tumor necrosis factor-alpha secretion. J Lipid Res, 46(5), 1027-37, 2005.
• Kingsley, PJ, Rouzer, CA, Saleh, S, Marnett, LJ Simultaneous analysis of prostaglandin glyceryl esters and prostaglandins by electrospray tandem mass spectrometry. Anal Biochem, 343(2), 203-11, 2005.
• Moth, CW, Prusakiewicz, JJ, Marnett, LJ, Lybrand, TP Stereoselective binding of indomethacin ethanolamide derivatives to cyclooxygenase-1. J Med Chem, 48(10), 3613-20, 2005.
• Rouzer, CA, Marnett, LJ Structural and functional differences between cyclooxygenases: fatty acid oxygenases with a critical role in cell signaling. Biochem Biophys Res Commun, 338(1), 34-44, 2005.
• Hata, AN, Lybrand, TP, Marnett, LJ, Breyer, RM Structural determinants of arylacetic acid nonsteroidal anti-inflammatory drugs necessary for binding and activation of the prostaglandin D2 receptor CRTH2. Mol Pharmacol, 67(3), 640-7, 2005.
• Wang, Hao, Marnett, Lawrence J, Harris, Thomas M, Rizzo, Carmelo J A novel synthesis of malondialdehyde adducts of deoxyguanosine, deoxyadenosine, and deoxycytidine. Chem Res Toxicol, 17(2), 144-9, 2004.
• Wang, H, Guo, Y, Wang, D, Kingsley, PJ, Marnett, LJ, Das, SK, DuBois, RN, Dey, SK Aberrant cannabinoid signaling impairs oviductal transport of embryos. Nat Med, 10(10), 1074-80, 2004.
• K.R. Kozak, J.J. Prusakiewicz, S.W. Rowlinson, D.R. Prudhomme, and L.J. Marnett. "Amino Acid Determinants in Cyclooxygenase-2 Oxygenation of the Endocannabinoid Anandamide." Biochemistry , 429041-9049, 2004.
• Rouzer, Carol A., Kingsley, Phillip J., Wang, Haibin, Zhang, Hao, Morrow, Jason D., Dey, Sudhansu K., Marnett, Lawrence J. Cyclooxygenase-1-dependent prostaglandin synthesis modulates tumor necrosis factor alpha secretion in lipopolysachharide-challenged murine resident peritoneal macrophages. J Biol Chem, 2004.
• Hashim, MF, Riggins, JN, Schnetz-Boutaud, N, Voehler, M, Stone, MP, Marnett, LJ In vitro bypass of malondialdehyde-deoxyguanosine adducts: differential base selection during extension by the Klenow fragment of DNA polymerase I is the critical determinant of replication outcome. Biochemistry, 43(37), 11828-35, 2004.
• West, James D, Ji, Chuan, Duncan, Stephen T, Amarnath, Venkataraman, Schneider, Claus, Rizzo, Carmelo J, Brash, Alan R, Marnett, Lawrence J Induction of apoptosis in colorectal carcinoma cells treated with 4-hydroxy-2-nonenal and structurally related aldehydic products of lipid peroxidation. Chem Res Toxicol, 17(4), 453-62, 2004.
• Riggins, James, Daniels, J., Rouzer, Carol, Marnett, Lawrence Kinetic and Thermodynamic Analysis of the Hydrolytic Ring-Opening of the Malondialdehyde-Deoxyguanosine Adduct, 3-(2''-Deoxy-beta-d-erythro-pentofuranosyl)- pyrimido[1,2-alpha]purin-10(3H)-one. J Am Chem Soc, 126(26), 8237-8243, 2004.
• Riggins, JN, Pratt, DA, Voehler, M, Daniels, JS, Marnett, LJ Kinetics and mechanism of the general-acid-catalyzed ring-closure of the malondialdehyde-DNA adduct, N2-(3-oxo-1-propenyl)deoxyguanosine (N2OPdG-), to 3-(2''-Deoxy-beta-D-erythro-pentofuranosyl)pyrimido[1,2-alpha]purin- 10(3H)-one (M1dG). J Am Chem Soc, 126(34), 10571-81, 2004.
• Cline, Susan D, Riggins, James N, Tornaletti, Silvia, Marnett, Lawrence J, Hanawalt, Philip C Malondialdehyde adducts in DNA arrest transcription by T7 RNA polymerase and mammalian RNA polymerase II. Proc Natl Acad Sci U S A, 101(19), 7275-80, 2004.
• J.J. Prusakiewicz, A.S. Felts, B.S. Mackenzie, and L.J. Marnett. "Molecular Basis of the Time-Dependent Inhibition of Cyclooxygenases by Indomethacin." Biochemistry, 4315439-15445, 2004.
• Prusakiewicz, JJ, Felts, AS, Mackenzie, BS, Marnett, LJ Molecular basis of the time-dependent inhibition of cyclooxygenases by indomethacin. Biochemistry, 43(49), 15439-45, 2004.
• Kozak, Kevin R, Prusakiewicz, Jeffery J, Marnett, Lawrence J Oxidative metabolism of endocannabinoids by COX-2. Curr Pharm Des, 10(6), 659-67, 2004.
• R.P. Remmel, B.C. Crews, K.R. Kozak, A.S. Kalgutkar, and L.J. Marnett. "Studies on the Metabolism of the Novel, Selective Cyclooxygenase-2 Inhibitor, Indomethacin Phenethylamide in Rat, Mouse, and Human Liver Microsomes: Identification of Active Metabolites." Drug Metab. Dispos., 32113-122, 2004.
• Remmel, RP, Crews, BC, Kozak, KR, Kalgutkar, AS, Marnett, LJ, Algutkar, AS Studies on the metabolism of the novel, selective cyclooxygenase-2 inhibitor indomethacin phenethylamide in rat, mouse, and human liver microsomes: identification of active metabolites. Drug Metab Dispos, 32(1), 113-22, 2004.
• Nirodi, Chaitanya S, Crews, Brenda C, Kozak, Kevin R, Morrow, Jason D, Marnett, Lawrence J The glyceryl ester of prostaglandin E2 mobilizes calcium and activates signal transduction in RAW264.7 cells. Proc Natl Acad Sci U S A, 101(7), 1840-5, 2004.
• Rowlinson, Scott W, Kiefer, James R, Prusakiewicz, Jeffery J, Pawlitz, Jennifer L, Kozak, Kevin R, Kalgutkar, Amit S, Stallings, William C, Kurumbail, Ravi G, Marnett, Lawrence J A novel mechanism of cyclooxygenase-2 inhibition involving interactions with Ser-530 and Tyr-385. J Biol Chem, 278(46), 45763-9, 2003.
• Kozak, Kevin R, Prusakiewicz, Jeffery J, Rowlinson, Scott W, Prudhomme, Daniel R, Marnett, Lawrence J Amino Acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid anandamide. Biochemistry, 42(30), 9041-9, 2003.
• Kingsley, Philip J, Marnett, Lawrence J Analysis of endocannabinoids by Ag(+) coordination tandem mass spectrometry. Anal Biochem, 314(1), 8-15, 2003.
• Otteneder, M, Scott Daniels, J, Voehler, M, Marnett, LJ Development of a method for determination of the malondialdehyde-deoxyguanosine adduct in urine using liquid chromatography-tandem mass spectrometry. Anal Biochem, 315(2), 147-51, 2003.
• Marnett, Lawrence J, Riggins, James N, West, James D Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein. J Clin Invest, 111(5), 583-93, 2003.
• Phillip Hochgesang, G, Nemeth-Cawley, Jennifer F, Rowlinson, Scott W, Caprioli, Richard M, Marnett, Lawrence J Functional analysis of the molecular determinants of cyclooxygenase-2 acetylation by 2-acetoxyphenylhept-2-ynyl sulfide. Arch Biochem Biophys, 409(1), 127-33, 2003.
• VanderVeen, Laurie A, Hashim, Muhammed F, Shyr, Yu, Marnett, Lawrence J Induction of frameshift and base pair substitution mutations by the major DNA adduct of the endogenous carcinogen malondialdehyde. Proc Natl Acad Sci U S A, 100(24), 14247-52, 2003.
• Aronoff, David M, Boutaud, Olivier, Marnett, Lawrence J, Oates, John A Inhibition of prostaglandin H2 synthases by salicylate is dependent on the oxidative state of the enzymes. J Pharmacol Exp Ther, 304(2), 589-95, 2003.
• Niedernhofer, Laura J., Daniels, John S., Rouzer, Carol A., Greene, Rachel E., Marnett, Lawrence J Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cellsi. J Biol Chem, 278(), 31426-31433, 2003.
• Rouzer, Carol A, Marnett, Lawrence J Mechanism of free radical oxygenation of polyunsaturated fatty acids by cyclooxygenases. Chem Rev, 103(6), 2239-304, 2003.
• Yu, Z, Schneider, C, Boeglin, WE, Marnett, LJ, Brash, AR The lipoxygenase gene ALOXE3 implicated in skin differentiation encodes a hydroperoxide isomerase. Proc Natl Acad Sci U S A, 100(16), 9162-7, 2003.
• Kozak, Kevin R, Gupta, Rajnish A, Moody, John S, Ji, Chuan, Boeglin, William E, DuBois, Raymond N, Brash, Alan R, Marnett, Lawrence J 15-Lipoxygenase metabolism of 2-arachidonylglycerol. Generation of a peroxisome proliferator-activated receptor alpha agonist. J Biol Chem, 277(26), 23278-86, 2002.
• A.S. Kalgutkar, S.W. Rowlinson, B.C. Crews, and L.J. Marnett. "Amide Derivatives of Meclofenamic Acid as Selective Cyclooxygenase-2 Inhibitors." Bioorg. Med. Chem. Lett, 12521-524, 2002.
• Kalgutkar, AS, Rowlinson, SW, Crews, BC, Marnett, LJ Amide derivatives of meclofenamic acid as selective cyclooxygenase-2 inhibitors. Bioorg Med Chem Lett, 12(4), 521-4, 2002.
• Marnett, Lawrence J, DuBois, Raymond N COX-2: a target for colon cancer prevention. Annu Rev Pharmacol Toxicol, 4255-80, 2002.
• C.A. Rouzer, K. Ghebreselasi, and L.J. Marnett "Chemical Stability of 2-Arachidonylglycerol under Biological Conditions." Chem. Phys. Lipids , 11969-82, 2002.
• Rouzer, CA, Ghebreselasie, K, Marnett, LJ Chemical stability of 2-arachidonylglycerol under biological conditions. Chem Phys Lipids, 119(1-2), 69-82, 2002.
• Schneider, C, Boeglin, WE, Prusakiewicz, JJ, Rowlinson, SW, Marnett, LJ, Samel, N, Brash, AR Control of prostaglandin stereochemistry at the 15-carbon by cyclooxygenases-1 and -2. A critical role for serine 530 and valine 349. J Biol Chem, 277(1), 478-85, 2002.
• Burleigh, ME, Babaev, VR, Oates, JA, Harris, RC, Gautam, S, Riendeau, D, Marnett, LJ, Morrow, JD, Fazio, S, Linton, MF Cyclooxygenase-2 promotes early atherosclerotic lesion formation in LDL receptor-deficient mice. Circulation, 105(15), 1816-23, 2002.
• Boutaud, Olivier, Aronoff, David M, Richardson, Jacob H, Marnett, Lawrence J, Oates, John A Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H(2) synthases. Proc Natl Acad Sci U S A, 99(10), 7130-5, 2002.
• Plastaras, JP, Dedon, PC, Marnett, LJ Effects of DNA structure on oxopropenylation by the endogenous mutagens malondialdehyde and base propenal. Biochemistry, 41(15), 5033-42, 2002.
• Kozak, Kevin R, Prusakiewicz, Jeffery J, Rowlinson, Scott W, Marnett, Lawrence J Enantiospecific, selective cyclooxygenase-2 inhibitors. Bioorg Med Chem Lett, 12(9), 1315-8, 2002.
• S.L. Timofeevski, J.J. Prusakiewicz, C.A. Rouzer, and L.J. Marnett. "Isoform-Selective Interaction of Cyclooxygenase-2 with Indomethacin Amides Studied by Real-Time Fluorescence, Inhibition Kinetics, and Site-Directed Mutagenesis." Biochemistry, 419654-9662, 2002.
• Timofeevski, SL, Prusakiewicz, JJ, Rouzer, CA, Marnett, LJ Isoform-selective interaction of cyclooxygenase-2 with indomethacin amides studied by real-time fluorescence, inhibition kinetics, and site-directed mutagenesis. Biochemistry, 41(30), 9654-62, 2002.
• J.S. Moody and L.J. Marnett "Kinetics of Inhibition of Leukocyte 12-Lipoxygenase by the Isoform-Specific Inhibitor 4-(2-Oxapentadeca-4-yne)-phenylpropanoic Acid." Biochemistry, 4110297-10303, 2002.
• Moody, JS, Marnett, LJ Kinetics of inhibition of leukocyte 12-lipoxygenase by the isoform-specific inhibitor 4-(2-oxapentadeca-4-yne)phenylpropanoic acid. Biochemistry, 41(32), 10297-303, 2002.
• Kozak, Kevin R, Crews, Brenda C, Morrow, Jason D, Wang, Lee-Ho, Ma, Y Henry, Weinander, Rolf, Jakobsson, Per-Johan, Marnett, Lawrence J Metabolism of the endocannabinoids, 2-arachidonylglycerol and anandamide, into prostaglandin, thromboxane, and prostacyclin glycerol esters and ethanolamides. J Biol Chem, 277(47), 44877-85, 2002.
• Kozak, KR, Marnett, LJ Oxidative metabolism of endocannabinoids. Prostaglandins Leukot Essent Fatty Acids, 66(2-3), 211-20, 2002.
• L.J. Marnett "Oxy Radicals, Lipid Peroxidation and DNA Damage." Toxicology, 181-182219-222, 2002.
• Marnett, LJ Oxy radicals, lipid peroxidation and DNA damage. Toxicology, 181-182219-22, 2002.
• Otteneder, M, Plastaras, JP, Marnett, LJ Reaction of malondialdehyde-DNA adducts with hydrazines-development of a facile assay for quantification of malondialdehyde equivalents in DNA. Chem Res Toxicol, 15(3), 312-8, 2002.
• Marnett, LJ Recent developments in cyclooxygenase inhibition. Prostaglandins Other Lipid Mediat, 68-69153-64, 2002.
• Sekhar, KR, Spitz, DR, Harris, S, Nguyen, TT, Meredith, MJ, Holt, JT, Gius, D, Marnett, LJ, Summar, ML, Freeman, ML, Guis, D Redox-sensitive interaction between KIAA0132 and Nrf2 mediates indomethacin-induced expression of gamma-glutamylcysteine synthetase. Free Radic Biol Med, 32(7), 650-62, 2002.
• Prusakiewicz, Jeffery J, Kingsley, Philip J, Kozak, Kevin R, Marnett, Lawrence J Selective oxygenation of N-arachidonylglycine by cyclooxygenase-2. Biochem Biophys Res Commun, 296(3), 612-7, 2002.
• Weisenseel, JP, Reddy, GR, Marnett, LJ, Stone, MP Structure of an oligodeoxynucleotide containing a 1,N(2)-propanodeoxyguanosine adduct positioned in a palindrome derived from the Salmonella typhimurium hisD3052 gene: Hoogsteen pairing at pH 5.2. Chem Res Toxicol, 15(2), 127-39, 2002.
• Weisenseel, JP, Reddy, GR, Marnett, LJ, Stone, MP Structure of the 1,N(2)-propanodeoxyguanosine adduct in a three-base DNA hairpin loop derived from a palindrome in the Salmonella typhimurium hisD3052 gene. Chem Res Toxicol, 15(2), 140-52, 2002.
• Ji, C, Amarnath, V, Pietenpol, JA, Marnett, LJ 4-hydroxynonenal induces apoptosis via caspase-3 activation and cytochrome c release. Chem Res Toxicol, 14(8), 1090-6, 2001.
• K.R. Kozak, J.J. Prusakiewicz, S.W. Rowlinson, C. Schneider, and L.J. Marnett. "Amino Acid Determinants in Cyclooxygenase-2 Oxygenation of the Endocannabinoid 2-Arachidonyl-glycerol." J. Biol. Chem, 27630072-30077, 2001.
• Kozak, KR, Prusakiewicz, JJ, Rowlinson, SW, Schneider, C, Marnett, LJ Amino acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid 2-arachidonylglycerol. J Biol Chem, 276(32), 30072-7, 2001.
• Nemeth, JF, Hochgesang, GP, Marnett, LJ, Caprioli, RM, Hochensang, GP Characterization of the glycosylation sites in cyclooxygenase-2 using mass spectrometry. Biochemistry, 40(10), 3109-16, 2001.
• Boutaud, O, Brame, CJ, Chaurand, P, Li, J, Rowlinson, SW, Crews, BC, Ji, C, Marnett, LJ, Caprioli, RM, Roberts, LJ, Oates, JA Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid. Biochemistry, 40(23), 6948-55, 2001.
• Kurumbail, R G, Kiefer, J R, Marnett, L J Cyclooxygenase enzymes: catalysis and inhibition. Curr Opin Struct Biol, 11(6), 752-60, 2001.
• Marnett, LJ, Plastaras, JP Endogenous DNA damage and mutation. Trends Genet, 17(4), 214-21, 2001.
• Schnetz-Boutaud, NC, Chapeau, MC, Marnett, LJ Enzymatic synthesis of M(1)G-deoxyribose. Curr Protoc Nucleic Acid Chem, Chapter 1Unit1.2, 2001.
• VanderVeen, LA, Hashim, MF, Nechev, LV, Harris, TM, Harris, CM, Marnett, LJ Evaluation of the mutagenic potential of the principal DNA adduct of acrolein. J Biol Chem, 276(12), 9066-70, 2001.
• Ji, C, Kozak, KR, Marnett, LJ IkappaB kinase, a molecular target for inhibition by 4-hydroxy-2-nonenal. J Biol Chem, 276(21), 18223-8, 2001.
• Kozak, KR, Crews, BC, Ray, JL, Tai, HH, Morrow, JD, Marnett, LJ Metabolism of prostaglandin glycerol esters and prostaglandin ethanolamides in vitro and in vivo. J Biol Chem, 276(40), 36993-8, 2001.
• Riggins, JN, Marnett, LJ Mutagenicity of the malondialdehyde oligomerization products 2-(3''-oxo-1''-propenyl)-malondialdehyde and 2,4-dihydroxymethylene-3-(2,2-dimethoxyethyl)glutaraldehyde in Salmonella. Mutat Res, 497(1-2), 153-7, 2001.
• Boutaud, O, Li, J, Chaurand, P, Brame, CJ, Marnett, LJ, Roberts, LJ, Oates, JA Oxygenation of arachidonic acid by cyclooxygenases generates reactive intermediates that form adducts with proteins. Adv Exp Med Biol, 500133-7, 2001.
• Moody, JS, Kozak, KR, Ji, C, Marnett, LJ Selective oxygenation of the endocannabinoid 2-arachidonylglycerol by leukocyte-type 12-lipoxygenase. Biochemistry, 40(4), 861-6, 2001.
• Schnetz-Boutaud, NC, Saleh, S, Marnett, LJ, Stone, MP Structure of the malondialdehyde deoxyguanosine adduct M1G when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene. Adv Exp Med Biol, 500513-6, 2001.
• Schnetz-Boutaud, NC, Saleh, S, Marnett, LJ, Stone, MP The exocyclic 1,N2-deoxyguanosine pyrimidopurinone M1G is a chemically stable DNA adduct when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene. Biochemistry, 40(51), 15638-49, 2001.
• Fink, SP, Marnett, LJ The relative contribution of adduct blockage and DNA repair on template utilization during replication of 1,N2-propanodeoxyguanosine and pyrimido. Mutat Res, 485(3), 209-18, 2001.
• Kalgutkar, AS, Crews, BC, Rowlinson, SW, Marnett, AB, Kozak, KR, Remmel, RP, Marnett, LJ Biochemically based design of cyclooxygenase-2 (COX-2) inhibitors: facile conversion of nonsteroidal antiinflammatory drugs to potent and highly selective COX-2 inhibitors. Proc Natl Acad Sci U S A, 97(2), 925-30, 2000.
• Marnett, LJ Cyclooxygenase mechanisms. Curr Opin Chem Biol, 4(5), 545-52, 2000.
• Kalgutkar, AS, Marnett, AB, Crews, BC, Remmel, RP, Marnett, LJ Ester and amide derivatives of the nonsteroidal antiinflammatory drug, indomethacin, as selective cyclooxygenase-2 inhibitors. J Med Chem, 43(15), 2860-70, 2000.
• Lanzo, CA, Sutin, J, Rowlinson, S, Talley, J, Marnett, LJ Fluorescence quenching analysis of the association and dissociation of a diarylheterocycle to cyclooxygenase-1 and cyclooxygenase-2: dynamic basis of cyclooxygenase-2 selectivity. Biochemistry, 39(20), 6228-34, 2000.
• Kozak, KR, Rowlinson, SW, Marnett, LJ Oxygenation of the endocannabinoid, 2-arachidonylglycerol, to glyceryl prostaglandins by cyclooxygenase-2. J Biol Chem, 275(43), 33744-9, 2000.
• Marnett, LJ Oxyradicals and DNA damage. Carcinogenesis, 21(3), 361-70, 2000.
• Schnetz-Boutaud, N, Daniels, JS, Hashim, MF, Scholl, P, Burrus, T, Marnett, LJ Pyrimido[1,2-alpha]purin-10(3H)-one: a reactive electrophile in the genome. Chem Res Toxicol, 13(10), 967-70, 2000.
• Plastaras, JP, Riggins, JN, Otteneder, M, Marnett, LJ Reactivity and mutagenicity of endogenous DNA oxopropenylating agents: base propenals, malondialdehyde, and N(epsilon)-oxopropenyllysine. Chem Res Toxicol, 13(12), 1235-42, 2000.
• Marnett, LJ, Wright, TL, Crews, BC, Tannenbaum, SR, Morrow, JD Regulation of prostaglandin biosynthesis by nitric oxide is revealed by targeted deletion of inducible nitric-oxide synthase. J Biol Chem, 275(18), 13427-30, 2000.
• Rowlinson, SW, Crews, BC, Goodwin, DC, Schneider, C, Gierse, JK, Marnett, LJ Spatial requirements for 15-(R)-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid synthesis within the cyclooxygenase active site of murine COX-2. Why acetylated COX-1 does not synthesize 15-(R)-hete. J Biol Chem, 275(9), 6586-91, 2000.
• Marnett, LJ Structure, function and inhibition of cyclo-oxygenases. Ernst Schering Res Found Workshop, (31), 65-83, 2000.
• Goodwin, DC, Rowlinson, SW, Marnett, LJ Substitution of tyrosine for the proximal histidine ligand to the heme of prostaglandin endoperoxide synthase 2: implications for the mechanism of cyclooxygenase activation and catalysis. Biochemistry, 39(18), 5422-32, 2000.
• Schnetz-Boutaud, NC, Mao, H, Stone, MP, Marnett, LJ Synthesis of oligonucleotides containing the alkali-labile pyrimidopurinone adduct, M(1)G. Chem Res Toxicol, 13(2), 90-5, 2000.
• Plastaras, JP, Guengerich, FP, Nebert, DW, Marnett, LJ Xenobiotic-metabolizing cytochromes P450 convert prostaglandin endoperoxide to hydroxyheptadecatrienoic acid and the mutagen, malondialdehyde. J Biol Chem, 275(16), 11784-90, 2000.
• Marnett, LJ, Rowlinson, SW, Goodwin, DC, Kalgutkar, AS, Lanzo, CA Arachidonic acid oxygenation by COX-1 and COX-2. Mechanisms of catalysis and inhibition. J Biol Chem, 274(33), 22903-6, 1999.
• Marnett, LJ Chemistry and biology of DNA damage by malondialdehyde. IARC Sci Publ, (150), 17-27, 1999.
• Marnett, LJ, Kalgutkar, AS Cyclooxygenase 2 inhibitors: discovery, selectivity and the future. Trends Pharmacol Sci, 20(11), 465-9, 1999.
• Kalgutkar, AS, Crews, BC, Rowlinson, SW, Garner, C, Marnett, LJ Discovery of a new class of selective cyclooxygenase-2 (COX-2) inhibitor that covalently modifies the isozyme. Adv Exp Med Biol, 469139-43, 1999.
• Mao, H, Schnetz-Boutaud, NC, Weisenseel, JP, Marnett, LJ, Stone, MP Duplex DNA catalyzes the chemical rearrangement of a malondialdehyde deoxyguanosine adduct. Proc Natl Acad Sci U S A, 96(12), 6615-20, 1999.
• Goodwin, DC, Landino, LM, Marnett, LJ Effects of nitric oxide and nitric oxide-derived species on prostaglandin endoperoxide synthase and prostaglandin biosynthesis. FASEB J, 13(10), 1121-36, 1999.
• Montine, TJ, Sidell, KR, Crews, BC, Markesbery, WR, Marnett, LJ, Roberts, LJ, Morrow, JD Elevated CSF prostaglandin E2 levels in patients with probable AD. Neurology, 53(7), 1495-8, 1999.
• Marnett, LJ Lipid peroxidation-DNA damage by malondialdehyde. Mutat Res, 424(1-2), 83-95, 1999.
• Richards, KM, Moody, JS, Marnett, LJ Mechanism of inhibition of porcine leukocyte 12-lipoxygenase by the isoform-specific inhibitor 4-(2-oxapentadeca-4-yne)phenylpropanoic acid. Biochemistry, 38(50), 16529-38, 1999.
• Johnson, KA, Mierzwa, ML, Fink, SP, Marnett, LJ MutS recognition of exocyclic DNA adducts that are endogenous products of lipid oxidation. J Biol Chem, 274(38), 27112-8, 1999.
• Goodwin, DC, Landino, LM, Marnett, LJ Reactions of prostaglandin endoperoxide synthase with nitric oxide and peroxynitrite. Drug Metab Rev, 31(1), 273-94, 1999.
• Mao, H, Reddy, GR, Marnett, LJ, Stone, MP Solution structure of an oligodeoxynucleotide containing the malondialdehyde deoxyguanosine adduct N2-(3-oxo-1-propenyl)-dG (ring-opened M1G) positioned in a (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene. Biochemistry, 38(41), 13491-501, 1999.
• Rowlinson, SW, Crews, BC, Lanzo, CA, Marnett, LJ The binding of arachidonic acid in the cyclooxygenase active site of mouse prostaglandin endoperoxide synthase-2 (COX-2). A putative L-shaped binding conformation utilizing the top channel region. J Biol Chem, 274(33), 23305-10, 1999.
• Daniel, TO, Liu, H, Morrow, JD, Crews, BC, Marnett, LJ Thromboxane A2 is a mediator of cyclooxygenase-2-dependent endothelial migration and angiogenesis. Cancer Res, 59(18), 4574-7, 1999.
• Kalgutkar, AS, Crews, BC, Rowlinson, SW, Garner, C, Seibert, K, Marnett, LJ Aspirin-like molecules that covalently inactivate cyclooxygenase-2. Science, 280(5367), 1268-70, 1998.
• Kalgutkar, AS, Kozak, KR, Crews, BC, Hochgesang, GP, Marnett, LJ Covalent modification of cyclooxygenase-2 (COX-2) by 2-acetoxyphenyl alkyl sulfides, a new class of selective COX-2 inactivators. J Med Chem, 41(24), 4800-18, 1998.
• Marnett, LJ, Kalgutkar, AS Design of selective inhibitors of cyclooxygenase-2 as nonulcerogenic anti-inflammatory agents. Curr Opin Chem Biol, 2(4), 482-90, 1998.
• Ji, C, Rouzer, CA, Marnett, LJ, Pietenpol, JA Induction of cell cycle arrest by the endogenous product of lipid peroxidation, malondialdehyde. Carcinogenesis, 19(7), 1275-83, 1998.
• Lanzo, CA, Beechem, JM, Talley, J, Marnett, LJ Investigation of the binding of isoform-selective inhibitors to prostaglandin endoperoxide synthases using fluorescence spectroscopy. Biochemistry, 37(1), 217-26, 1998.
• Langouët, S, Mican, AN, Müller, M, Fink, SP, Marnett, LJ, Muhle, SA, Guengerich, FP Misincorporation of nucleotides opposite five-membered exocyclic ring guanine derivatives by escherichia coli polymerases in vitro and in vivo: 1,N2-ethenoguanine, 5,6,7,9-tetrahydro-9-oxoimidazo[1, 2-a]purine, and 5,6,7,9-tetrahydro-7-hydroxy-9-oxoimidazo[1, 2-a]purine. Biochemistry, 37(15), 5184-93, 1998.
• Goodwin, DC, Gunther, MR, Hsi, LC, Crews, BC, Eling, TE, Mason, RP, Marnett, LJ Nitric oxide trapping of tyrosyl radicals generated during prostaglandin endoperoxide synthase turnover. Detection of the radical derivative of tyrosine 385. J Biol Chem, 273(15), 8903-9, 1998.
• Rouzer, CA, Chaudhary, AK, Nokubo, M, Ferguson, DM, Reddy, GR, Blair, IA, Marnett, LJ Analysis of the malondialdehyde-2''-deoxyguanosine adduct pyrimidopurinone in human leukocyte DNA by gas chromatography/electron capture/negative chemical ionization/mass spectrometry. Chem Res Toxicol, 10(2), 181-8, 1997.
• Ji, C, Marnett, LJ, Pietenpol, JA Cell cycle re-entry following chemically-induced cell cycle synchronization leads to elevated p53 and p21 protein levels. Oncogene, 15(22), 2749-53, 1997.
• Kalgutkar, AS, Crews, BC, Marnett, LJ Inactivation of prostaglandin endoperoxide synthase (PGHS) by N-(substituted)maleimides. Adv Exp Med Biol, 40779-85, 1997.
• Smith, CJ, Morrow, JD, Roberts, LJ, Marnett, LJ Induction of prostaglandin endoperoxide synthase-1 (COX-1) in a human promonocytic cell line by treatment with the differentiating agent TPA. Adv Exp Med Biol, 400A99-106, 1997.
• Richards, KM, Marnett, LJ Leukocyte 12-lipoxygenase: expression, purification, and investigation of the role of methionine residues in turnover-dependent inactivation and 5,8,11,14-eicosatetraynoic acid inhibition. Biochemistry, 36(22), 6692-9, 1997.
• Fink, SP, Reddy, GR, Marnett, LJ Mutagenicity in Escherichia coli of the major DNA adduct derived from the endogenous mutagen malondialdehyde. Proc Natl Acad Sci U S A, 94(16), 8652-7, 1997.
• Landino, LM, Crews, BC, Gierse, JK, Hauser, SD, Marnett, LJ Mutational analysis of the role of the distal histidine and glutamine residues of prostaglandin-endoperoxide synthase-2 in peroxidase catalysis, hydroperoxide reduction, and cyclooxygenase activation. J Biol Chem, 272(34), 21565-74, 1997.
• Johnson, KA, Fink, SP, Marnett, LJ Repair of propanodeoxyguanosine by nucleotide excision repair in vivo and in vitro. J Biol Chem, 272(17), 11434-8, 1997.
• Hashim, MF, Schnetz-Boutaud, N, Marnett, LJ Replication of template-primers containing propanodeoxyguanosine by DNA polymerase beta. Induction of base pair substitution and frameshift mutations by template slippage and deoxynucleoside triphosphate stabilization. J Biol Chem, 272(32), 20205-12, 1997.
• Niedernhofer, LJ, Riley, M, Schnetz-Boutaud, N, Sanduwaran, G, Chaudhary, AK, Reddy, GR, Marnett, LJ Temperature-dependent formation of a conjugate between tris(hydroxymethyl)aminomethane buffer and the malondialdehyde-DNA adduct pyrimidopurinone. Chem Res Toxicol, 10(5), 556-61, 1997.
• Chaudhary, AK, Reddy, GR, Blair, IA, Marnett, LJ Characterization of an N6-oxopropenyl-2''-deoxyadenosine adduct in malondialdehyde-modified DNA using liquid chromatography/electrospray ionization tandem mass spectrometry. Carcinogenesis, 17(5), 1167-70, 1996.
• Kalgutkar, AS, Crews, BC, Marnett, LJ Design, synthesis, and biochemical evaluation of N-substituted maleimides as inhibitors of prostaglandin endoperoxide synthases. J Med Chem, 39(8), 1692-703, 1996.
• Smith, CJ, Marnett, LJ Effects of cysteine-to-serine mutations on structural and functional properties of prostaglandin endoperoxide synthase. Arch Biochem Biophys, 335(2), 342-50, 1996.
• Kalgutkar, AS, Crews, BC, Marnett, LJ Kinetics of the interaction of nonsteroidal antiinflammatory drugs with prostaglandin endoperoxide synthase-1 studied by limited proteolysis. Biochemistry, 35(28), 9076-82, 1996.
• Landino, LM, Marnett, LJ Mechanism of hydroperoxide reduction by mangano-prostaglandin endoperoxide synthase. Biochemistry, 35(8), 2637-43, 1996.
• Reddy, GR, Marnett, LJ Mechanism of reaction of beta-(aryloxy)acroleins with nucleosides. Chem Res Toxicol, 9(1), 12-5, 1996.
• Landino, LM, Crews, BC, Timmons, MD, Morrow, JD, Marnett, LJ Peroxynitrite, the coupling product of nitric oxide and superoxide, activates prostaglandin biosynthesis. Proc Natl Acad Sci U S A, 93(26), 15069-74, 1996.
• Fink, SP, Reddy, GR, Marnett, LJ Relative contribution of cytosine deamination and error-prone replication to the induction of propanodeoxyguanosine-->deoxyadenosine mutations in Escherichia coli. Chem Res Toxicol, 9(1), 277-83, 1996.
• Hashim, MF, Marnett, LJ Sequence-dependent induction of base pair substitutions and frameshifts by propanodeoxyguanosine during in vitro DNA replication. J Biol Chem, 271(15), 9160-5, 1996.
• Kingma, PS, Corbett, AH, Burcham, PC, Marnett, LJ, Osheroff, N Abasic sites stimulate double-stranded DNA cleavage mediated by topoisomerase II. DNA lesions as endogenous topoisomerase II poisons. J Biol Chem, 270(37), 21441-4, 1995.
• Marnett, LJ Aspirin and related nonsteroidal anti-inflammatory drugs as chemopreventive agents against colon cancer. Prev Med, 24(2), 103-6, 1995.
• Benamira, M, Johnson, K, Chaudhary, A, Bruner, K, Tibbetts, C, Marnett, LJ Induction of mutations by replication of malondialdehyde-modified M13 DNA in Escherichia coli: determination of the extent of DNA modification, genetic requirements for mutagenesis, and types of mutations induced. Carcinogenesis, 16(1), 93-9, 1995.
• Weisenseel, JP, Moe, JG, Reddy, GR, Marnett, LJ, Stone, MP Structure of a duplex oligodeoxynucleotide containing propanodeoxyguanosine opposite a two-base deletion in the (CpG)3 frame shift hotspot of Salmonella typhimurium hisD3052 determined by 1H NMR and restrained molecular dynamics. Biochemistry, 34(1), 50-64, 1995.
• Marnett, LJ, Wilcox, AL The chemistry of lipid alkoxyl radicals and their role in metal-amplified lipid peroxidation. Biochem Soc Symp, 6165-72, 1995.
• Moe, JG, Reddy, GR, Marnett, LJ, Stone, MP 1H NMR characterization of a duplex oligodeoxynucleotide containing propanodeoxyguanosine opposite a two-base deletion in the (CpG)3 frameshift hotspot of Salmonella typhimurium hisD3052. Chem Res Toxicol, 7(3), 319-28, 1994.
• Chaudhary, AK, Nokubo, M, Marnett, LJ, Blair, IA Analysis of the malondialdehyde-2''-deoxyguanosine adduct in rat liver DNA by gas chromatography/electron capture negative chemical ionization mass spectrometry. Biol Mass Spectrom, 23(8), 457-64, 1994.
• Marnett, LJ DNA adducts of alpha,beta-unsaturated aldehydes and dicarbonyl compounds. IARC Sci Publ, (125), 151-63, 1994.
• Chaudhary, AK, Nokubo, M, Reddy, GR, Yeola, SN, Morrow, JD, Blair, IA, Marnett, LJ Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver. Science, 265(5178), 1580-2, 1994.
• Reddy, RG, Yoshimoto, T, Yamamoto, S, Funk, CD, Marnett, LJ Expression of porcine leukocyte 12-lipoxygenase in a baculovirus/insect cell system and its characterization. Arch Biochem Biophys, 312(1), 219-26, 1994.
• Reddy, RG, Yoshimoto, T, Yamamoto, S, Marnett, LJ Expression, purification, and characterization of porcine leukocyte 12-lipoxygenase produced in the methylotrophic yeast, Pichia pastoris. Biochem Biophys Res Commun, 205(1), 381-8, 1994.
• Marnett, LJ Generation of mutagens during arachidonic acid metabolism. Cancer Metastasis Rev, 13(3-4), 303-8, 1994.
• Kennedy, TA, Smith, CJ, Marnett, LJ Investigation of the role of cysteines in catalysis by prostaglandin endoperoxide synthase. J Biol Chem, 269(44), 27357-64, 1994.
• Marnett, LJ, Ji, C Modulation of oxidant formation in mouse skin in vivo by tumor-promoting phorbol esters. Cancer Res, 54(7 Suppl), 1886s-1889s, 1994.
• Marnett, LJ, Honn, KV Overview of articles on eicosanoids and cancer. Cancer Metastasis Rev, 13(3-4), 237-9, 1994.
• Beckman, JK, Bagheri, F, Ji, C, Blair, IA, Marnett, LJ Phospholipid peroxidation in tumor promoter-exposed mouse skin. Carcinogenesis, 15(12), 2937-44, 1994.
• Kalgutkar, AS, Marnett, LJ Rapid inactivation of prostaglandin endoperoxide synthases by N-(carboxyalkyl)maleimides. Biochemistry, 33(29), 8625-8, 1994.
• Burcham, PC, Marnett, LJ Site-specific mutagenesis by a propanodeoxyguanosine adduct carried on an M13 genome. J Biol Chem, 269(46), 28844-50, 1994.
• Singh, US, Moe, JG, Reddy, GR, Weisenseel, JP, Marnett, LJ, Stone, MP 1H NMR of an oligodeoxynucleotide containing a propanodeoxyguanosine adduct positioned in a (CG)3 frameshift hotspot of Salmonella typhimurium hisD3052: Hoogsteen base-pairing at pH 5.8. Chem Res Toxicol, 6(6), 825-36, 1993.
• Benamira, M, Marnett, LJ Construction of a vector for site-specific frameshift mutagenesis containing the mutable hotspot of Salmonella typhimurium TA98 on an M13 bacteriophage. Chem Res Toxicol, 6(3), 317-27, 1993.
• Smith, CJ, Morrow, JD, Roberts, LJ, Marnett, LJ Differentiation of monocytoid THP-1 cells with phorbol ester induces expression of prostaglandin endoperoxide synthase-1 (COX-1). Biochem Biophys Res Commun, 192(2), 787-93, 1993.
• Marnett, LJ, Burcham, PC Endogenous DNA adducts: potential and paradox. Chem Res Toxicol, 6(6), 771-85, 1993.
• Wells, I, Marnett, LJ Inactivation of prostaglandin endoperoxide synthase by acylating derivatives of indomethacin. Biochemistry, 32(10), 2710-6, 1993.
• Wilcox, AL, Marnett, LJ Polyunsaturated fatty acid alkoxyl radicals exist as carbon-centered epoxyallylic radicals: a key step in hydroperoxide-amplified lipid peroxidation. Chem Res Toxicol, 6(4), 413-6, 1993.
• Pal, I, Odenwaller, R, Marnett, LJ 7-Oxabicycloheptylprostanoic acids: potent, time-dependent cyclooxygenase inhibitors that induce a conformational change in the prostaglandin endoperoxide synthase protein. J Med Chem, 35(12), 2340-2, 1992.
• Jajoo, HK, Burcham, PC, Goda, Y, Blair, IA, Marnett, LJ A thermospray liquid chromatography/mass spectrometry method for analysis of human urine for the major malondialdehyde-guanine adduct. Chem Res Toxicol, 5(6), 870-5, 1992.
• Wells, I, Marnett, LJ Acetylation of prostaglandin endoperoxide synthase by N-acetylimidazole: comparison to acetylation by aspirin. Biochemistry, 31(40), 9520-5, 1992.
• Marnett, LJ Aspirin and the potential role of prostaglandins in colon cancer. Cancer Res, 52(20), 5575-89, 1992.
• Odenwaller, R, Maddipati, KR, Marnett, LJ Detection of a higher oxidation state of manganese-prostaglandin endoperoxide synthase. J Biol Chem, 267(20), 13863-9, 1992.
• Ji, C, Marnett, LJ Oxygen radical-dependent epoxidation of (7S,8S)-dihydroxy-7,8-dihydrobenzo[a]pyrene in mouse skin in vivo. Stimulation by phorbol esters and inhibition by antiinflammatory steroids. J Biol Chem, 267(25), 17842-8, 1992.
• Benamira, M, Singh, U, Marnett, LJ Site-specific frameshift mutagenesis by a propanodeoxyguanosine adduct positioned in the (CpG)4 hot-spot of Salmonella typhimurium hisD3052 carried on an M13 vector. J Biol Chem, 267(31), 22392-400, 1992.
• Benamira, M, Marnett, LJ The lipid peroxidation product 4-hydroxynonenal is a potent inducer of the SOS response. Mutat Res, 293(1), 1-10, 1992.
• Marnett, LJ, Leithauser, MT, Richards, KM, Blair, I, Honn, KV, Yamamoto, S, Yoshimoto, T Arachidonic acid metabolism of cytosolic fractions of Lewis lung carcinoma cells. Adv Prostaglandin Thromboxane Leukot Res, 21B895-900, 1991.
• Chapeau, MC, Marnett, LJ Enzymatic synthesis of purine deoxynucleoside adducts. Chem Res Toxicol, 4(6), 636-8, 1991.
• Goda, Y, Marnett, LJ High-performance liquid chromatography with electrochemical detection for determination of the major malondialdehyde-guanine adduct. Chem Res Toxicol, 4(5), 520-4, 1991.
• Marnett, LJ Peroxyl free radicals: biological reactive intermediates produced during lipid oxidation. Adv Exp Med Biol, 28365-70, 1991.
• Odenwaller, R, O''Gara, CY, Marnett, LJ Properties of trypsin-cleaved PGH synthase. Adv Prostaglandin Thromboxane Leukot Res, 21A81-4, 1991.
• Marnett, LJ, Reddy, A, Pruess-Schwartz, D, Ji, C Oxidation of polycyclic hydrocarbons by oxygen radicals. Princess Takamatsu Symp, 2163-73, 1990.
• Marnett, LJ Prostaglandin synthase-mediated metabolism of carcinogens and a potential role for peroxyl radicals as reactive intermediates. Environ Health Perspect, 885-12, 1990.
• Stone, K, Uzieblo, A, Marnett, LJ Studies of the reaction of malondialdehyde with cytosine nucleosides. Chem Res Toxicol, 3(5), 467-72, 1990.
• Shimada, T, Martin, MV, Pruess-Schwartz, D, Marnett, LJ, Guengerich, FP Roles of individual human cytochrome P-450 enzymes in the bioactivation of benzo(a)pyrene, 7,8-dihydroxy-7,8-dihydrobenzo(a)pyrene, and other dihydrodiol derivatives of polycyclic aromatic hydrocarbons. Cancer Res, 49(22), 6304-12, 1989.
• Capdevila, J, Gil, L, Orellana, M, Marnett, LJ, Mason, JI, Yadagiri, P, Falck, JR Inhibitors of cytochrome P-450-dependent arachidonic acid metabolism. Arch Biochem Biophys, 261(2), 257-63, 1988.