The VICC.ORG Investigator Directory
Zhaozhong Han, Ph.D.
Assistant Professor
VICC Member
Radiation Oncologist
Contact Information:
Vanderbilt Center for Radiation Oncology
1301 22nd Ave
Nashville, TN 37232-5671
615-322-1167
Fax: 615-343-0161
Research Specialty:
Biomarker discovery and development, Phage display, Protein engineering, Cellular and molecular imaging of cancer, Targeting drug delivery, Cancer stem cells, Therapeutic proteins
Research Description:
The past decades have witnessed the dramatic development in molecular targeted therapy for cancers. Treatments approved for targeting molecular events within cancers include those modulate cell survival, proliferation, invasion, metastasis and angiogenesis. However, patients respond to the molecular targeted therapy with a broad spectrum of efficacy because of the biological heterogeneity of cancers. It is increasingly important to understand systematically how cancer cells respond to the treatment so that the tumor responsiveness can be monitored and the personalized treatment can be justified in time. This lab is devoted to investigate and develop such treatment-inducible molecular signatures within tumors for cancer imaging, drug delivery and drug discovery. Currently, we are interested in how cancers respond to tyrosine kinase inhibitors (TKIs) and ionizing radiation (IR). Ongoing projects in this lab include: 1) biomarker discovery. We are using phage-displayed peptides, engineered protein scaffolds and antibody fragments to profile the TKIs/IR-induced changes in the plasma membrane proteome of cancer cells; 2) molecular and cellular imaging to assess tumor response to TKIs/IR treatment; 3) tumor-targeted drug delivery; 4) biological function of tax-interacting protein 1 (TIP-1) within cancer cells, especially its roles in cancer cell responsiveness to TKIs/IR treatment; 4) roles of cancer stem cells in response of breast cancers to TKIs/IR treatment. The long term goal is to develop tools and rationales to personalize molecular targeted therapy for cancer patients.
Publications:
- Wang, H, Yan, H, Fu, A, Han, M, Hallahan, D, Han, Z TIP-1 Translocation onto the Cell Plasma Membrane Is a Molecular Biomarker of Tumor Response to Ionizing Radiation. PLoS One, 5(8), 2010.
- Jaboin, JJ, Han, Z, Hallahan, DE Using in vivo biopanning for the development of radiation-guided drug delivery systems. Methods Mol Biol, 542285-300, 2009.
- Han, Z, Fu, A, Wang, H, Diaz, R, Geng, L, Onishko, H, Hallahan, DE Noninvasive assessment of cancer response to therapy. Nat Med, 14(3), 343-9, 2008.
- Hariri, G, Zhang, Y, Fu, A, Han, Z, Brechbiel, M, Tantawy, MN, Peterson, TE, Mernaugh, R, Hallahan, D Radiation-guided P-selectin antibody targeted to lung cancer. Ann Biomed Eng, 36(5), 821-30, 2008.
- Han, Z, Wang, H, Hallahan, DE Radiation-guided gene therapy of cancer. Technol Cancer Res Treat, 5(4), 437-44, 2006.
- Han ZZ, Hariri G, Hallahan DE "Radiotherapy and tumor-targeted drug delivery." “Multimodal Concepts for Integration of Cytotoxic Drugs and Radiation Therapy”. Edited by Brown JM, Mehta MP and Nieder C. , Springer Press. 151-162, 2006.
- Karatan E, Han ZZ, Kay BK "Molecular Display Technologies." In “Encyclopedia of Molecular Cell Biology and Molecular Medicine”, Wiley(8), 8:409-420, 2005.
- Han ZZ, Karatan E, and Kay BK "Protein Interaction Networks." in “Phage Display in Biotechnology and Drug Discovery”. Edited by Sidhu SS, CRC Press, Baton Rouge, FL. 321-346, 2005.
- Han, Z, Karatan, E, Scholle, MD, McCafferty, J, Kay, BK Accelerated screening of phage-display output with alkaline phosphatase fusions. Comb Chem High Throughput Screen, 7(1), 55-62, 2004.
- Kwon, Y, Han, Z, Karatan, E, Mrksich, M, Kay, BK Antibody arrays prepared by cutinase-mediated immobilization on self-assembled monolayers. Anal Chem, 76(19), 5713-20, 2004.
- Han, Z, Simpson, JT, Fivash, MJ, Fisher, R, Mori, T Identification and characterization of peptides that bind to cyanovirin-N, a potent human immunodeficiency virus-inactivating protein. Peptides, 25(4), 551-61, 2004.
- Karatan, E, Merguerian, M, Han, Z, Scholle, MD, Koide, S, Kay, BK Molecular recognition properties of FN3 monobodies that bind the Src SH3 domain. Chem Biol, 11(6), 835-44, 2004.
- Wasiak, S, Denisov, AY, Han, Z, Leventis, PA, de Heuvel, E, Boulianne, GL, Kay, BK, Gehring, K, McPherson, PS Characterization of a gamma-adaptin ear-binding motif in enthoprotin. FEBS Lett, 555(3), 437-42, 2003.
- Han, Z, Xiong, C, Mori, T, Boyd, MR Discovery of a stable dimeric mutant of cyanovirin-N (CV-N) from a T7 phage-displayed CV-N mutant library. Biochem Biophys Res Commun, 292(4), 1036-43, 2002.
- Mori, T, Barrientos, LG, Han, Z, Gronenborn, AM, Turpin, JA, Boyd, MR Functional homologs of cyanovirin-N amenable to mass production in prokaryotic and eukaryotic hosts. Protein Expr Purif, 26(1), 42-9, 2002.
- Barrientos, LG, Louis, JM, Botos, I, Mori, T, Han, Z, O''Keefe, BR, Boyd, MR, Wlodawer, A, Gronenborn, AM The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures. Structure, 10(5), 673-86, 2002.
